1887

Abstract

A Gram-positive, rod-shaped, spore-forming bacterium (PAT 05) was isolated from the rhizosphere of the perennial shrub in north-eastern Patagonia, Argentina. Its overall biochemical and physiological characteristics indicated that this strain should be placed in the alkaliphilic group. Strain PAT 05 grew at pH 7–10 (optimum pH 8), but not at pH 6. Its DNA G+C content was 39·7 mol%. Sequence analysis of the 16S rRNA gene of PAT 05 revealed the closest match (99·6 % similarity) with sp. DSM 8714. The highest level of DNA–DNA relatedness (88·6 %) was also found with this strain. On the basis of 16S rRNA gene sequence similarity and phylogenetic analysis, G+C content and DNA–DNA hybridization data, strain PAT 05 is related at the species level to sp. DSM 8714, a member of a group referred as phenon 4a by Nielsen . [Nielsen, P., Fritze, D. & Priest, F. G. (1995). , 1745–1761] , which still lacks taxonomic standing. These results support the proposal of strain PAT 05 (=DSM 16117=ATCC BAA-965) as the type strain of sp. nov.

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2005-01-01
2019-10-14
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vol. , part 1, pp. 443 - 447

Electron photomicrograph of a negatively stained cell of strain PAT 05 grown at pH 10 showing peritrichous flagellation. Bar, 1 µm.



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