1887

Abstract

The phylogeny of human isolates of species was estimated based on partial sequences of four separate housekeeping genes. The clustering of each set of sequences was in accordance with speciation of the strains with few exceptions: of 108 gene fragments examined, only three appeared to have been subject to recombination events across the species barrier. Housekeeping gene similarity supported previous DNA–DNA hybridization data for the genus rather than the phylogeny inferred from 16S rRNA gene sequence comparison. The similarity of sequences of with those of suggested preservation of the former species in the genus . Three strains representing a novel taxon were unique with respect to the four investigated gene loci. 16S rRNA gene sequence analysis suggested that this taxon belonged to the Parainfluenzae cluster. DNA–DNA hybridization data supported this generic placement. Nine strains of the novel taxon were available for analysis. They were distinct from representatives of all current species of the genus by conventional phenotypic characterization. Genotypic and phenotypic data show that the strains merit recognition as a novel species of . The name sp. nov. is proposed, with HK 85 (=CCUG 48703=NCTC 13334) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63325-0
2005-01-01
2019-11-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550449.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63325-0&mimeType=html&fmt=ahah

References

  1. Angen, Ø., Mutters, R., Caugant, D. A., Olsen, J. E. & Bisgaard, M. ( 1999; ). Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA–DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida spec. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov. Int J Syst Bacteriol 49, 67–86.[CrossRef]
    [Google Scholar]
  2. Angen, Ø., Ahrens, P., Kuhnert, P., Christensen, H. & Mutters, R. ( 2003; ). Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedisHaemophilus somnus’, ‘Haemophilus agni’ and ‘Histophilus ovis’. Int J Syst Evol Microbiol 53, 1449–1456.[CrossRef]
    [Google Scholar]
  3. Burbach, S. ( 1987; ). Reklassifizierung der Gattung Haemophilus Winslow et al. 1917 auf Grund der DNA-Basensequenzhomologie. PhD dissertation, Philipps-Universität Marburg, Germany.
  4. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  5. Casin, I., Grimont, F., Grimont, P. A. D. & Sanson-Le Pors, M. J. ( 1985; ). Lack of deoxyribonucleic acid relatedness between Haemophilus ducreyi and other Haemophilus species. Int J Syst Bacteriol 35, 23–25.[CrossRef]
    [Google Scholar]
  6. Casin, I., Grimont, F. & Grimont, P. A. ( 1986; ). Deoxyribonucleic acid relatedness between Haemophilus aegyptius and Haemophilus influenzae. Ann Inst Pasteur Microbiol 137B, 155–163.
    [Google Scholar]
  7. Christensen, H., Bisgaard, M., Bojesen, A. M., Mutters, R. & Olsen, J. E. ( 2003; ). Genetic relationships among avian isolates classified as Pasteurella haemolytica, ‘Actinobacillus salpingitidis’ or Pasteurella anatis with proposal of Gallibacterium anatis gen. nov., comb. nov. and description of additional genomospecies within Gallibacterium gen. nov. Int J Syst Evol Microbiol 53, 275–287.[CrossRef]
    [Google Scholar]
  8. Christensen, H., Bisgaard, M., Aalbaek, B. & Olsen, J. E. ( 2004; ). Reclassification of Bisgaard taxon 33, with proposal of Volucribacter psittacicida gen. nov., sp. nov. and Volucribacter amazonae sp. nov. as new members of the Pasteurellaceae. Int J Syst Evol Microbiol 54, 813–818.[CrossRef]
    [Google Scholar]
  9. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 143–153.[CrossRef]
    [Google Scholar]
  10. Dewhirst, F. E., Paster, B. J. & Bright, P. L. ( 1989; ). Chromobacterium, Eikenella, Kingella, Neisseria, Simonsiella, and Vitreoscilla species comprise a major branch of the beta group Proteobacteria by 16S ribosomal ribonucleic acid sequence comparison: transfer of Eikenella and Simonsiella to the family Neisseriaceae (emend.). Int J Syst Bacteriol 39, 258–266.[CrossRef]
    [Google Scholar]
  11. Dewhirst, F. E., Paster, B. J., Olsen, I. & Fraser, G. J. ( 1992; ). Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J Bacteriol 174, 2002–2013.
    [Google Scholar]
  12. Escara, J. F. & Hutton, J. R. ( 1980; ). Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19, 1315–1327.[CrossRef]
    [Google Scholar]
  13. Foster, G., Ross, H. M., Malnick, H., Willems, A., Hutson, R. A., Reid, R. J. & Collins, M. D. ( 2000; ). Phocoenobacter uteri gen. nov., sp. nov., a new member of the family Pasteurellaceae Pohl (1979) 1981 isolated from a harbour porpoise (Phocoena phocoena). Int J Syst Evol Microbiol 50, 135–139.[CrossRef]
    [Google Scholar]
  14. Garrity, G. M., Bell, J. A. & Lilburn, T. G. ( 2003; ). Taxonomic outline of the procaryotes. In Bergey's Manual of Systematic Bacteriology, 2nd edn, release 4.0, pp. 1–3. New York: Springer. doi:10.1007/bergeysoutline200310
  15. Hedegaard, J., Okkels, H., Bruun, B., Kilian, M., Mortensen, K. K. & Nørskov-Lauritsen, N. ( 2001; ). Phylogeny of the genus Haemophilus as determined by comparison of partial infB sequences. Microbiology 147, 2599–2609.
    [Google Scholar]
  16. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  17. Jahnke, K. D. ( 1992; ). Basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef]
    [Google Scholar]
  18. Kilian, M. ( 1976; ). A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol 93, 9–62.[CrossRef]
    [Google Scholar]
  19. Kilian, M. ( 2003; ). Haemophilus. In Manual of Clinical Microbiology, 8th edn, pp. 623–635. Edited by P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller & R. H. Yolken. Washington, DC: American Society for Microbiology.
  20. Kilian, M. ( 2004; ). Genus Haemophilus Winslow, Broadhurst, Buchanan, Rogers and Smith 1917, 561AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer (in press).
  21. Kilian, M., Mestecky, J. & Schrohenloher, R. E. ( 1979; ). Pathogenic species of Haemophilus and Streptococcus pneumoniae produce immunoglobulin A1 protease. Infect Immun 26, 143–149.
    [Google Scholar]
  22. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  23. May, B. J., Zhang, Q., Li, L. L., Paustian, M. L., Whittam, T. S. & Kapur, V. ( 2001; ). Complete genomic sequence of Pasteurella multocida, Pm70. Proc Natl Acad Sci U S A 98, 3460–3465.[CrossRef]
    [Google Scholar]
  24. Meats, E., Feil, E. J., Stringer, S., Cody, A. J., Goldstein, R., Kroll, J. S., Popovic, T. & Spratt, B. G. ( 2003; ). Characterization of encapsulated and nonencapsulated Haemophilus influenzae and determination of phylogenetic relationships by multilocus sequence typing. J Clin Microbiol 41, 1623–1636.[CrossRef]
    [Google Scholar]
  25. Mutters, R., Mannheim, W. & Bisgaard, M. ( 1989; ). Taxonomy of the group. In Pasteurella and Pasteurellosis, pp. 3–34. Edited by C. Adlam & J. M. Rutter. London: Academic Press.
  26. Nørskov-Lauritsen, N., Christensen, H., Okkels, H., Kilian, M. & Bruun, B. ( 2004; ). Delineation of the genus Actinobacillus by comparison of partial infB sequences. Int J Syst Evol Microbiol 54, 635–644.[CrossRef]
    [Google Scholar]
  27. Olsen, I., Dewhirst, F. E., Paster, B. J. & Busse, H.-J. ( 2004; ). Family Pasteurellaceae Pohl 1981, 382VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer (in press).
  28. Osawa, R., Rainey, F., Fujisawa, T., Lang, E., Busse, H. J., Walsh, T. P. & Stackebrandt, E. ( 1995; ). Lonepinella koalarum gen. nov., sp. nov., a new tannin-protein complex degrading bacterium. Syst Appl Microbiol 18, 368–373.[CrossRef]
    [Google Scholar]
  29. Paster, B. J. & Dewhirst, F. E. ( 1988; ). Phylogeny of campylobacters, wolinellas, Bacteroides gracilis, and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int J Syst Bacteriol 38, 56–62.[CrossRef]
    [Google Scholar]
  30. Potts, T. V., Zambon, J. J. & Genco, R. J. ( 1985; ). Reassignment of Actinobacillus actinomycetemcomitans to the genus Haemophilus as Haemophilus actinomycetemcomitans comb. nov. Int J Syst Bacteriol 35, 337–341.[CrossRef]
    [Google Scholar]
  31. Potts, T. V., Mitra, T., O'Keefe, T., Zambon, J. J. & Genco, R. J. ( 1986; ). Relationships among isolates of oral haemophili as determined by DNA-DNA hybridization. Arch Microbiol 145, 136–141.[CrossRef]
    [Google Scholar]
  32. Selander, R. K., Caugant, D. A., Ochman, H., Musser, J. M., Gilmour, M. N. & Whittam, T. S. ( 1986; ). Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol 51, 873–884.
    [Google Scholar]
  33. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63325-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63325-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error