1887

Abstract

A haloalkaliphilic Gram-positive bacterium, strain 6AG, that grew aerobically at an optimum temperature of 37 °C and at pH 7–10 (optimum 9·0), was isolated from algal mat from a mineral pool located in Malvizza in the Campania region (southern Italy). The isolate tolerated high concentrations of NaCl, up to 25 %. On the basis of 16S rRNA gene sequence similarity, the strain was shown to belong to the genus . Analysis of the 16S rRNA gene sequence revealed high similarity between strain 6AG and an unidentified isolate from Hailaer soda lake (China) (99·9 % identity) and two Kenyan isolates, 3E1 and WE4 (98·3 and 97·8 % identity, respectively). The G+C content of the DNA was 48·4 mol%. The predominant respiratory quinones were MK-7(H), MK-7(H) and DMK-7(H); phosphatidylglycerol and diphosphatidylglycerol were the predominant polar lipids. iC15 : 0 and aiC15 : 0 were the major fatty acids. Strain 6AG accumulated osmolytes. The phylogenetic distance of strain 6AG (=DSM 15402=ATCC BAA-957) from any recognized species within the genus allowed it to be classified as the type strain of sp. nov.

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2005-01-01
2019-12-06
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