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Abstract

A Gram-negative, non-motile, non-spore-forming, short rod-shaped bacterium (UST950701-009P) was isolated from a marine biofilm in Hong Kong waters. Colonies are pink in colour, convex with a smooth surface and entire edge. Brown diffusible pigment is produced. Whitish colonies, with otherwise identical morphology, emerge from every culture upon ageing. The white colonies can be maintained as separate cultures (UST950701-009W) without turning pink. UST950701-009P and UST950701-009W have identical 16S rRNA gene sequences and similar G+C (65·9–66·2 mol%) and fatty acid (86·22–88·52 % 18 : 17) contents. Phylogenetic analysis of the 16S rRNA gene sequence places UST950701-009P within the group of the -subclass of the . The nearest neighbours belong to the genus , with similarity values ranging from 94·5 to 95·5 %. Data on G+C and fatty acid contents support the affiliation to the genus . UST950701-009P and -009W are heterotrophic, strictly aerobic and require NaCl for growth (2·0–14·0 %). Both grow in pH 5·0–10·0 and at 8–44 °C. Both are positive in oxidase, catalase and -galactosidase tests, but they differ in the pattern of carbohydrate oxidation and assimilation. Molecular evidence together with phenotypic characteristics shows that UST950701-009P constitutes a novel species within the genus . The name sp. nov. is proposed; the type strain is UST950701-009P (=NRRL B-41039=JCM 12479) and a morphovar is UST950701-009W (=NRRL B-41040=JCM 12480).

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2004-11-01
2020-01-27
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Neighbour-joining dendrogram showing the estimated phylogenetic relationships among UST950701-009P , UST950701-009W and related genera in the alpha-subclass of the on the basis of 16S rRNA gene sequences. species were chosen as the outgroup. Bar, 1 nucleotide substitution per 100 nucleotides. GenBank accession numbers are shown in parentheses. [PDF](48 KB)

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Strains UST950701-009P (a) and UST950701-009W (b) visualized using a scanning electron microscope.

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