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Abstract

A Gram-negative, non-motile, non-spore-forming, short rod-shaped bacterium (UST950701-009P) was isolated from a marine biofilm in Hong Kong waters. Colonies are pink in colour, convex with a smooth surface and entire edge. Brown diffusible pigment is produced. Whitish colonies, with otherwise identical morphology, emerge from every culture upon ageing. The white colonies can be maintained as separate cultures (UST950701-009W) without turning pink. UST950701-009P and UST950701-009W have identical 16S rRNA gene sequences and similar G+C (65·9–66·2 mol%) and fatty acid (86·22–88·52 % 18 : 17) contents. Phylogenetic analysis of the 16S rRNA gene sequence places UST950701-009P within the group of the -subclass of the . The nearest neighbours belong to the genus , with similarity values ranging from 94·5 to 95·5 %. Data on G+C and fatty acid contents support the affiliation to the genus . UST950701-009P and -009W are heterotrophic, strictly aerobic and require NaCl for growth (2·0–14·0 %). Both grow in pH 5·0–10·0 and at 8–44 °C. Both are positive in oxidase, catalase and -galactosidase tests, but they differ in the pattern of carbohydrate oxidation and assimilation. Molecular evidence together with phenotypic characteristics shows that UST950701-009P constitutes a novel species within the genus . The name sp. nov. is proposed; the type strain is UST950701-009P (=NRRL B-41039=JCM 12479) and a morphovar is UST950701-009W (=NRRL B-41040=JCM 12480).

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2004-11-01
2024-12-10
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References

  1. Acar J. F. 1980; The disc susceptibility test. In Antibiotics in Laboratory Medicine pp  24–54 Edited by Lorian V. Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Baumann P., Baumann L. 1988; The marine gram-negative eubacteria: genera Photobacterium Beneckea , Alteromonas , Pseudomonas and Alcaligenes . In The Prokaryotes , vol. 1. pp  1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. Berlin: Springer;
  3. Cottrell M. T., Kirchman D. L. 2000; Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Appl Environ Microbiol 66:5116–5122 [CrossRef]
    [Google Scholar]
  4. Hagström A., Pommier T., Rohwer F., Simu K., Stolte W., Svensson D., Zweifel U. L. 2002; Use of 16S ribosomal DNA for delineation of marine bacterioplankton species. Appl Environ Microbiol 68:3628–3633 [CrossRef]
    [Google Scholar]
  5. Lafay B., Ruimy R., Rausch de Traubenberg C., Breittmayer V., Gauthier M. J., Christen R. 1995; Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima . Int J Syst Bacteriol 45:290–296 [CrossRef]
    [Google Scholar]
  6. Ludwig W., Strunk O., Westram R. 29 other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  7. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  8. Neu B., Voigt A., Mitlohner R. 7 other authors 2001; Biological cells as templates for hollow microcapsules. J Microencapsul 18:385–395 [CrossRef]
    [Google Scholar]
  9. Rosenblum B. B., Lee L. G., Spurgeon S. L., Khan S. H., Menchen S. M., Heiner C. R., Chen S. M. 1997; New dye-labeled terminators for improved DNA sequencing patterns. Nucleic Acids Res 25:4500–4504 [CrossRef]
    [Google Scholar]
  10. Smibert R. M., Krieg N. R. 1994; Phenotypic characteristics. In Methods for General and Molecular Biology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Van Trappen S., Mergaert J., Swings J. 2004; Loktanella salsilacus gen. nov., sp. nov., Loktanella fryxellensis sp. nov. and Loktanella vestfoldensis sp. nov. new members of the Roseobacter group, isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54:1263–1269 [CrossRef]
    [Google Scholar]
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