1887

Abstract

The taxonomic position of a bacterium isolated from a cold desert of the Himalayas, India, was analysed by using a polyphasic approach. The isolated strain, designated K22-03, had phenotypic characteristics that matched those of the genus and it represents a novel species. The almost-complete 16S rRNA gene sequence (1464 bases) of the novel strain was compared with those of previously studied type strains and confirmed that the strain belongs to the genus . 16S rRNA gene sequence analysis indicated that strain K22-03 differs from all other species of by at least 2·5 %. DNA–DNA hybridization showed that it had low genomic relatedness with (MTCC 3704, 23 %), (MTCC 3812, 61 %), (MTCC 3854, 45 %) and (MTCC 3703, 51 %), the four species with which it was most closely related based on 16S rRNA gene sequence analysis (97–97·5 % similarity). Therefore, strain K22-03 should be recognized as a novel species, for which the name sp. nov. is proposed. The type strain is K22-03 (=MTCC 6226=DSM 16419=JCM 12481).

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2005-01-01
2021-04-23
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