1887

Abstract

The taxonomic position of a bacterium isolated from a cold desert of the Himalayas, India, was analysed by using a polyphasic approach. The isolated strain, designated K22-03, had phenotypic characteristics that matched those of the genus and it represents a novel species. The almost-complete 16S rRNA gene sequence (1464 bases) of the novel strain was compared with those of previously studied type strains and confirmed that the strain belongs to the genus . 16S rRNA gene sequence analysis indicated that strain K22-03 differs from all other species of by at least 2·5 %. DNA–DNA hybridization showed that it had low genomic relatedness with (MTCC 3704, 23 %), (MTCC 3812, 61 %), (MTCC 3854, 45 %) and (MTCC 3703, 51 %), the four species with which it was most closely related based on 16S rRNA gene sequence analysis (97–97·5 % similarity). Therefore, strain K22-03 should be recognized as a novel species, for which the name sp. nov. is proposed. The type strain is K22-03 (=MTCC 6226=DSM 16419=JCM 12481).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63290-0
2005-01-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550091.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63290-0&mimeType=html&fmt=ahah

References

  1. Alam, S. I., Singh, L., Dube, S., Reddy, G. S. N. & Shivaji, S. ( 2003; ). Psychrophilic Planococcus maitriensis sp.nov. from Antarctica. Syst Appl Microbiol 26, 505–510.[CrossRef]
    [Google Scholar]
  2. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  3. Engelhardt, M. A., Daly, K., Swannell, R. P. J. & Head, I. M. ( 2001; ). Isolation and characterization of a novel hydrocarbon-degrading, Gram-positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. nov. J Appl Microbiol 90, 237–247.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Hao, M. V. & Komagata, K. ( 1985; ). A new species of Planococcus, P. kocurii isolated from fish, frozen foods, and fish curing brine. J Gen Appl Microbiol 31, 441–455.[CrossRef]
    [Google Scholar]
  6. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  7. Migula, W. ( 1894; ). Uber ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1, 235–238.
    [Google Scholar]
  8. Minnikin, D. E. & Goodfellow, M. ( 1976; ). Lipid composition in the classification and identification of Nocardia and related taxa. In The Biology of the Nocardiaceae, pp. 160–219. Edited by M. Goodfellow, G. H. Brownell & J. A. Serrano. London: Academic Press.
  9. Minnikin, D. E., Collins, M. D. & Goodfellow, M. ( 1978; ). Menaquinone patterns in the classification of nocardioform and related bacteria. Zentbl Bakteriol Parasitenkd Infektionskr U HygI Abt 1 Suppl. 6, 85–90.
    [Google Scholar]
  10. Nakagawa, Y., Sakane, T. & Yokota, A. ( 1996; ). Emendation of the genus Planococcus and transfer of Flavobacterium okeanokoites Zobell and Upham 1944 to the genus Planococcus as Planococcus okeanokoites comb. nov. Int J Syst Bacteriol 46, 866–870.[CrossRef]
    [Google Scholar]
  11. Pandey, K. K., Mayilraj, S. & Chakrabarti, T. ( 2002; ). Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52, 1559–1567.[CrossRef]
    [Google Scholar]
  12. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef]
    [Google Scholar]
  13. Reddy, G. S. N., Aggarwal, R. K., Matsumoto, G. I. & Shivaji, S. ( 2000; ). Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50, 1553–1561.[CrossRef]
    [Google Scholar]
  14. Reddy, G. S. N., Prakash, J. S. S., Vairamani, M., Prabhakar, S., Matsumoto, G. I. & Shivaji, S. ( 2002; ). Planococcus antarcticus and Planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Extremophiles 6, 253–261.[CrossRef]
    [Google Scholar]
  15. Romano, I., Giordano, A., Lama, L., Nicolaus, B. & Gambacorta, A. ( 2003; ). Planococcus rifietensis sp. nov., isolated from algal mat collected from a sulfurous spring in Campania (Italy). Syst Appl Microbiol 26, 357–366.[CrossRef]
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Shivaji, S., Ray, M. K., Rao, N. S., Saisree, L., Jagannadham, M. V., Kumar, G. S., Reddy, G. S. N. & Bhargava, P. M. ( 1992; ). Sphingobacterium antarcticus sp. nov., a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42, 102–106.[CrossRef]
    [Google Scholar]
  18. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  19. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  20. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  21. Tourova, T. P. & Antonov, A. S. ( 1987; ). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19, 333–355.
    [Google Scholar]
  22. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  23. Yoon, J.-H., Weiss, N., Kang, K. H., Oh, T.-K. & Park, Y.-H. ( 2003; ). Planococcus maritimus sp. nov., isolated from sea water of a tidal flat in Korea. Int J Syst Evol Microbiol 53, 2013–2017.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63290-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63290-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error