1887

Abstract

A mycoplasma was isolated from the sputum of an immunodeficient patient with recurrent bronchitis. The isolate designated strain A39 was very fastidious and atypical for a mycoplasma in its colonial appearance. Classical biochemical tests for mycoplasma speciation could not differentiate the isolate from the pathogens and and serological identification as a recognized species was lacking. Specific PCR detection for these two species was negative. Subsequently, other strains were isolated from human patients that appeared to be similar to strain A39 in their physiological and genetic characteristics. Analysis of the 16S rRNA gene placed strain A39 and other isolates in the pneumoniae group of mycoplasmas, with the highest sequence similarity to (96·8 %), but with only 93·0 % similarity to and . Examination of the 16S–23S rRNA internally transcribed spacer sequence, protein electrophoresis profile, genome size and serological reactions indicated that this organism represents a novel species, for which the name sp. nov. is proposed, with strain A39 (=NCTC 11740=ATCC BAA-992) as the type strain.

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2005-11-01
2019-10-16
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