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Abstract

A bacterial isolate from the Baltic Sea, BA160, was characterized for its physiological and biochemical features, fatty acid profile, G+C content and phylogenetic position based on 16S rRNA gene sequences. The strain was isolated from the surface water of the central Baltic Sea during the decay of a plankton bloom. Phylogenetic analyses of the 16S rRNA gene sequence revealed a clear affiliation with the family ‘’, and showed the closest phylogenetic relationship with the species and . The G+C content of the DNA was 38·4 mol%. The strain was red-coloured due to carotenoids, Gram-negative, rod-shaped, and catalase- and oxidase-positive. Growth was observed at salinities from 0 to 6 %, with an optimum around 1·5 %. Temperature for growth ranged from 4 to 40 °C, with an optimum around 30 °C. The fatty acids were dominated by branched-chain fatty acids (>87 %), with a high abundance of iso-C (23 %) and anteiso-C (19 %). According to its morphology, physiology, fatty acid composition, G+C content and 16S rRNA gene sequence, strain BA160 is considered to represent a new genus of the family ‘’. Due to its aquatic origin, the name gen. nov, sp. nov. is suggested for the type species (type strain, BA160=DSM 16537=LMG 22565=CIP 108445) of the new genus.

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2004-11-01
2019-10-20
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References

  1. Atlas, R. M. ( 1993; ). Handbook of Microbiological Media. Edited by L. C. Parks. Boca Raton, FL: CRC Press.
  2. Bowman, J. P., Mancuso Nichols, C. & Gibson, J. A. E. ( 2003; ). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53, 1343–1355.[CrossRef]
    [Google Scholar]
  3. Brettar, I. & Höfle, M. G. ( 1993; ). Nitrous oxide producing heterotrophic bacteria from the water column of the central Baltic: abundance and molecular identification. Mar Ecol Prog Ser 94, 253–265.[CrossRef]
    [Google Scholar]
  4. Brettar, I. & Rheinheimer, G. ( 1992; ). Influence of carbon availability on denitrification in the water column of the central Baltic. Limnol Oceanogr 37, 1146–1163.[CrossRef]
    [Google Scholar]
  5. Brettar, I., Christen, R. & Höfle, M. G. ( 2002; ). Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. Int J Syst Evol Microbiol 52, 1851–1857.[CrossRef]
    [Google Scholar]
  6. Brettar, I., Christen, R. & Höfle, M. G. ( 2004; ). Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga–Flavobacterium–Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54, 65–70.[CrossRef]
    [Google Scholar]
  7. Cottrell, M. T. & Kirchman, D. L. ( 2000; ). Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Appl Environ Microbiol 66, 5116–5122.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1995; ). phylip (phylogeny inference package), version 3.57c. Department of Genetics, University of Washington, Seattle, USA.
  9. Gascuel, O. ( 1997; ). bionj: an improved version of the NJ algorithm based on a simple model of sequence data. Mol Biol Evol 14, 685–695.[CrossRef]
    [Google Scholar]
  10. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  11. Gosink, J. J., Woese, C. R. & Staley, J. T. ( 1998; ). Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga-Flavobacterium-Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48, 223–235.[CrossRef]
    [Google Scholar]
  12. Höfle, M. G. ( 1982; ). Glucose uptake of Cytophaga johnsonae studied in batch and chemostat culture. Arch Microbiol 133, 289–294.[CrossRef]
    [Google Scholar]
  13. Höfle, M. G. ( 1992; ). Bacterioplankton community structure and dynamics after large-scale release of nonindigenous bacteria as revealed by low-molecular-weight-RNA analysis. Appl Environ Microbiol 58, 3387–3394; erratum 59, 351.
    [Google Scholar]
  14. Höfle, M. G. & Brettar, I. ( 1995; ). Taxonomic diversity and metabolic activity of microbial communities in the water column of the central Baltic Sea. Limnol Oceanogr 40, 868–874.[CrossRef]
    [Google Scholar]
  15. Ludwig, W. & Klenk, H.-P. ( 2001; ). Overview: a phylogenetic backbone and taxonomic framework for procaryotic systematics. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 49–65. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  16. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  17. Moore, E. R. B., Mau, M., Arnscheidt, A., Böttger, E. C., Hutson, R. A., Collins, M. D., Van De Peer, Y., De Wachter, R. & Timmis, K. N. ( 1996; ). The determination and comparison of the 16S rRNA gene sequences of species of the genus Pseudomonas (sensu stricto) and estimation of the natural intrageneric relationships. Syst Appl Microbiol 19, 476–492.
    [Google Scholar]
  18. Moore, E. R. B., Arnscheidt, A., Krüger, A., Strömpl, C. & Mau, M. ( 1999; ). Simplified protocols for the preparation of genomic DNA from bacterial cultures. In Molecular Microbial Ecology Manual, 1.6.1, 1–15. Edited by A. D. L. Akkermans, J. D. van Elsas & F. J. de Bruijn. Dordrecht, the Netherlands: Kluwer Academic Press.
  19. Mullis, K. B. & Faloona, F. A. ( 1987; ). Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol 155, 335–350.
    [Google Scholar]
  20. Nedashkovskaya, O. I., Vancanneyt, M., Van Trappen, S. & 7 other authors ( 2004; ). Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004 . Int J Syst Evol Microbiol 54, 1757–1764.[CrossRef]
    [Google Scholar]
  21. Oppenheimer, C. H. & ZoBell, C. E. ( 1952; ). The growth and viability of sixty-three species of marine bacteria as influenced by hydrostatic pressure. J Mar Res 11, 10–18.
    [Google Scholar]
  22. Perrière, G. & Gouy, M. ( 1996; ). WWW-query: an on-line retrieval system for biological sequence banks. Biochimie 78, 364–369.[CrossRef]
    [Google Scholar]
  23. Pinhassi, J., Azam, F., Hemphala, J., Long, R. A., Martinez, J., Zweifel, U. L. & Hagström, A. ( 1999; ). Coupling between bacterioplankton species composition, population dynamics, and organic matter degradation. Aquat Microb Ecol 17, 13–26.[CrossRef]
    [Google Scholar]
  24. Raj, H. D. & Maloy, S. R. ( 1990; ). Proposal of Cyclobacterium marinus gen. nov., comb. nov. for a marine bacterium previously assigned to the genus Flectobacillus. Int J Syst Bacteriol 40, 337–347.[CrossRef]
    [Google Scholar]
  25. Reichenbach, H., Kohl, W. & Achenbach, H. ( 1981; ). The flexirubin pigments, chemosystematically useful compounds. In The Flavobacterium–Cytophaga Group, pp. 101–108. Edited by H. Reichenbach & O. B. Weeks. Weinheim: Verlag Chemie.
  26. Sasser, M. ( 1990; ). Identification of bacteria by the gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark: MIDI Inc.
  27. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  28. Urakami, T. & Komagata, K. ( 1986; ). Methanol-utilizing Ancylobacter strains and comparison of their cellular fatty acid compositions and quinone systems with those of Spirosoma, Flectobacillus, and Runella species. Int J Syst Bacteriol 36, 415–421.[CrossRef]
    [Google Scholar]
  29. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. ( 2004; ). Algoriphagus antarcticus sp. nov., a novel psychrophile from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54, 1969–1973.[CrossRef]
    [Google Scholar]
  30. Woese, C. R., Maloy, S., Mandelco, L. & Raj, H. D. ( 1990; ). Phylogenetic placement of the Sprirosomaceae. Syst Appl Microbiol 13, 19–23.[CrossRef]
    [Google Scholar]
  31. Yi, H. & Chun, J. ( 2004; ). Hongiella mannitolivorans gen. nov., sp. nov., Hongiella halophila sp. nov. and Hongiella ornithinivorans sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 157–162.[CrossRef]
    [Google Scholar]
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vol. , part 6, pp. 2335–2341

A phase-contrast micrograph of and a table giving details of API results for strain BA160 are available to download. [PDF](103KB)



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