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Abstract

A Gram-positive short rod isolated from a saline soil in China was characterized using a polyphasic approach. This actinobacterium grew over a wide salinity range [0–15 % NaCl, 0–20 % KCl and 0–30 % MgCl.6HO (w/v); optimum concentrations for growth were 5 % NaCl, 5–10 % KCl, 10 % MgCl.6HO]. The optimum growth temperature and pH were 28–30 °C and 7·0–8·0, respectively. Chemotaxonomic features (peptidoglycan-type B2 with glycolyl residues; major menaquinones MK-10 and MK-11; predominating iso- and anteiso-branched cellular fatty acids; DNA G+C content 66·5 mol%) placed this organism within the genus . 16S rRNA gene sequence analysis confirmed this classification of the strain, but showed that it was distinct from its nearest neighbours. It formed a separate branch with type strains of the genus , and also shared low sequence similarity with them (<96 %). Based on phenotypic and molecular taxonomic results, it is proposed that the unknown isolate should be classified as a novel species in the genus , for which the name sp. nov. is proposed. The type strain is YIM 70130 (=KCTC 19017=CIP 108071).

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2005-01-01
2020-09-22
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