1887

Abstract

A group of 24 strains was isolated from deteriorated mural paintings situated in Spain (necropolis of Carmona) and Germany (church of Greene-Kreiensen). (GTG)-PCR genomic fingerprinting was performed on these strains to assess their genomic variability and the strains were delineated into four groups. Representatives were studied by 16S rRNA gene sequencing and were found to be closely related to and the species ‘’ (strain NCIMB 8796) and ‘’ (names not validly published) according to a search. The close similarity between , ‘’ NCIMB 8796, ‘’ and the mural painting isolates was confirmed by additional (GTG)-PCR, ARDRA, FAME and SDS-PAGE analyses. Furthermore, these techniques revealed that strains of ‘’, another name that has not been validly published, also showed high similarity to this group of organisms. On the other hand, it was shown that the strains labelled ‘’ in different collections do not all belong to the same species. Strain NCIMB 8796 can be allocated to , while strain DSM 54 (=ATCC 12905) shares the highest 16S rRNA gene sequence similarity with and (both around 98·6 %). On the basis of further DNA–DNA hybridization data and the study of phenotypic characteristics, one group of five mural painting strains was attributed to a novel species in the genus , for which the name sp. nov. is proposed. Finally, the remaining mural painting strains, one (LMG 18508=NCIMB 8796) of two strains belonging to ‘’ and strains belonging to ‘’ and ‘’ are allocated to the species and an emended description of is given.

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2005-01-01
2019-10-23
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vol. , part 1, pp. 119 - 131

Dendrogram based on UPGMA clustering of correlation coefficients of normalized 16S rRNA gene restriction profiles (ARDRA) of the type strain, ' ' strains LMG 18474 and LMG 18508, ' ', strains belonging to ' ' and additional isolates R-15936 and R-15943.

G+C content and DNA-DNA relatedness values of mural painting isolates, representative of (GTG) -groups A-D, the type strain, ' ' strains LMG 18474 and LMG 18508 and strains representing the named taxa ' ' and ' '.

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