1887

Abstract

A Gram-negative, motile, yellow-pigmented, slightly halophilic bacterial strain, SW-151, was isolated from sea water of the Yellow Sea in Korea, and subjected to a polyphasic taxonomic study. The isolate grew optimally at 30 °C and in the presence of 2–3 % NaCl. Strain SW-151 was characterized chemotaxonomically as having Q-10 as the predominant respiratory lipoquinone and C 7, C 7 and/or iso-C 2-OH and C 6 as the major fatty acids. Sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. The DNA G+C content was 58 mol%. 16S rRNA gene sequence analysis showed that strain SW-151 joins the evolutionary radiation enclosed by the genus . Similarities between the 16S rRNA gene sequences of strain SW-151 and the type strains of species ranged from 92·3 to 94·3 %, which is low enough to categorize strain SW-151 as a species distinct from previously described species. On the basis of phenotypic properties and phylogenetic distinctiveness, strain SW-151 (=KCTC 12232=DSM 16223) should be classified as a novel species, for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63218-0
2005-01-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550369.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63218-0&mimeType=html&fmt=ahah

References

  1. Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  2. Baumann, P. & Baumann, L. ( 1981; ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In The Prokaryotes, pp. 1302–1331. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer.
  3. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  4. Busse, H.-J., Denner, E. B. M., Buczolits, S., Salkinoja-Salonen, M., Bennasar, A. & Kämpfer, P. ( 2003; ). Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas. Int J Syst Evol Microbiol 53, 1253–1260.[CrossRef]
    [Google Scholar]
  5. Cohen-Bazire, G., Sistrom, W. R. & Stanier, R. Y. ( 1957; ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49, 25–68.[CrossRef]
    [Google Scholar]
  6. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  7. Fujii, K., Satomi, M., Morita, N., Motomura, T., Tanaka, T. & Kikuchi, S. ( 2003; ). Novosphingobium tardaugens sp. nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo. Int J Syst Evol Microbiol 53, 47–52.[CrossRef]
    [Google Scholar]
  8. Godoy, F., Vancanneyt, M., Martinez, M., Steinbüchel, A., Swings, J. & Rehm, B. H. A. ( 2003; ). Sphingopyxis chilensis sp. nov., a chlorophenol-degrading bacterium that accumulates polyhydroxyalkanoate, and transfer of Sphingomonas alaskensis to Sphingopyxis alaskensis comb. nov. Int J Syst Evol Microbiol 53, 473–477.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P., Denner, E. B. M., Meyer, S., Moore, E. R. B. & Busse, H.-J. ( 1997; ). Classification of “Pseudomonas azotocolligans” Anderson 1955, 132, in the genus Sphingomonas as Sphingomonas trueperi sp. nov. Int J Syst Bacteriol 47, 577–583.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Witzenberger, R., Denner, E. B. M., Busse, H.-J. & Neef, A. ( 2002; ). Sphingopyxis witflariensis sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 52, 2029–2034.[CrossRef]
    [Google Scholar]
  11. Komagata, K. & Suzuki, K. ( 1987; ). Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–203.
    [Google Scholar]
  12. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  13. Lee, J.-S., Shin, Y. K., Yoon, J.-H., Takeuchi, M., Pyun, Y.-R. & Park, Y.-H. ( 2001; ). Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov. and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water. Int J Syst Evol Microbiol 51, 1491–1498.
    [Google Scholar]
  14. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  15. Levring, T. ( 1946; ). Some culture experiments with Ulva and artificial seawater. Kungl Fysiogr Sallsk Lund Forh 16, 45–56.
    [Google Scholar]
  16. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  17. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. Newark, DE: MIDI.
  18. Sohn, J. H., Kwon, K. K., Kang, J.-H., Jung, H.-B. & Kim, S.-J. ( 2004; ). Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54, 1483–1487.[CrossRef]
    [Google Scholar]
  19. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  20. Staley, J. T. ( 1968; ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95, 1921–1942.
    [Google Scholar]
  21. Takeuchi, M., Kawai, F., Shimada, Y. & Yokota, A. ( 1993; ). Taxonomic study of polyethylene glycol-utilizing bacteria: emended description of the genus Sphingomonas and new descriptions of Sphingomonas macrogoltabidus sp. nov., Sphingomonas sanguis sp. nov. and Sphingomonas terrae sp. nov. Syst Appl Microbiol 16, 227–238.[CrossRef]
    [Google Scholar]
  22. Takeuchi, M., Sakane, T., Yanagi, M., Yamasato, K., Hamana, K. & Yokota, A. ( 1995; ). Taxonomic study of bacteria isolated from plants: proposal of Sphingomonas rosa sp. nov., Sphingomonas pruni sp. nov., Sphingomonas asaccharolytica sp. nov., and Sphingomonas mali sp. nov. Int J Syst Bacteriol 45, 334–341.[CrossRef]
    [Google Scholar]
  23. Takeuchi, M., Hamana, K. & Hiraishi, A. ( 2001; ). Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51, 1405–1417.
    [Google Scholar]
  24. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  25. Ushiba, Y., Takahara, Y. & Ohta, H. ( 2003; ). Sphingobium amiense sp. nov., a novel nonylphenol-degrading bacterium isolated from a river sediment. Int J Syst Evol Microbiol 53, 2045–2048.[CrossRef]
    [Google Scholar]
  26. Vancanneyt, M., Schut, F., Snauwaert, C., Goris, J., Swings, J. & Gottschal, J. C. ( 2001; ). Sphingomonas alaskensis sp. nov., a dominant bacterium from a marine oligotrophic environment. Int J Syst Evol Microbiol 51, 73–79.
    [Google Scholar]
  27. Yabuuchi, E., Yano, I., Oyaizu, H., Hashimoto, Y., Ezaki, T. & Yamamoto, H. ( 1990; ). Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulate comb. nov., and two genospecies of the genus Sphingomonas. Microbiol Immunol 34, 99–119.[CrossRef]
    [Google Scholar]
  28. Yabuuchi, E., Kosako, Y., Fujiwara, N., Naka, T., Matsunaga, I., Ogura, H. & Kobayashi, K. ( 2002; ). Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, Sphingobium, Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola. Int J Syst Evol Microbiol 52, 1485–1496.[CrossRef]
    [Google Scholar]
  29. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  30. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  31. Yoon, J.-H., Kim, H., Kim, I.-G., Kang, K. H. & Park, Y.-H. ( 2003; ). Erythrobacter flavus sp. nov., a slight halophile from the East Sea in Korea. Int J Syst Evol Microbiol 53, 1169–1174.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63218-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63218-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error