1887

Abstract

Two mesophilic anaerobic bacterial strains (Z7 and Z1) were isolated from waste water sludge of the Xinanzhang paper mill, Beijing, China. The strains were Gram-positive, non-spore-forming and motile. Cells were thin rods (0·2–0·4×4·0–8·0 μm). Growth of the strains was observed at 20–42 °C and pH 5·0–7·5. Both strains hydrolysed gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. The fermentation end products formed from glucose were acetate, ethanol, hydrogen and carbon dioxide. The predominant cellular fatty acids were the branched-chain fatty acids isoC (42·83 %) and isoC (32·11 %). The DNA G+C contents of strains Z7 and Z1 were 50·4 and 48·6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represent a new phyletic sublineage within the rRNA cluster, with <91 % 16S rRNA gene sequence similarity to currently described species. On the basis of polyphasic evidence from this study, gen. nov., sp. nov., a novel genus and species, is proposed, with strain Z7 (=JCM 12359=AS 1.5012) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63212-0
2004-11-01
2021-04-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs542257.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63212-0&mimeType=html&fmt=ahah

References

  1. Bryant M. P. 1986; Genus Ruminococcus . In Bergey's Manual of Systematic Bacteriology vol 2 pp  1093–1097 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Cato E. P., Gorge W. L., Finegold S. M. 1986; Genus Clostridium . In Bergey's Manual of Systematic Bacteriology vol. 2 pp  1141–1200 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium : proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826 [CrossRef]
    [Google Scholar]
  4. Duncan S. H., Hold G. L., Harmsen H. J. M., Stewart C. S., Flint H. J. 2002; Growth requirements and fermentation products of Fusobacterium prausnitzii , and a proposal to reclassify it as Faecalibacterium prausnitzii gen. nov., comb. nov.. Int J Syst Evol Microbiol 52:2141–2146 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1993 phylip (phylogenetic inference package), version 3.5c Department of Genetics, University of Washington; Seattle, WA, USA:
    [Google Scholar]
  7. He Y. L., Ding Y. F., Long Y. Q. 1991; Two cellulolytic Clostridium species: Clostridium cellulosi sp. nov. and Clostridium cellulofermentans sp. nov. Int J Syst Bacteriol 41:306–309 [CrossRef]
    [Google Scholar]
  8. Himelbloom B. H., Canale-Parola E. 1989; Clostridium methylpentosum sp. nov.: a ring-shaped intestinal bacterium that ferments only methylpentoses and pentoses. Arch Microbiol 151:287–293 [CrossRef]
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual , 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  10. Hungate R. E. 1969; A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B:117–132
    [Google Scholar]
  11. Kaneda T. 1991; Iso- and anteiso- fatty acids in bacteria: biosynthesis, function, and taxonomic significance. Microbiol Rev 55:288–302
    [Google Scholar]
  12. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  13. Lawson P. A., Song Y., Liu C., Molitoris D. R., Vaisanen M. L., Collins M. D., Finegold S. M. 2004; Anaerotruncus colihominis gen. nov., sp. nov. from human faeces. Int J Syst Evol Microbiol 54413–417 [CrossRef]
    [Google Scholar]
  14. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy , Special Publication 6 pp  227–291 Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4:109–118
    [Google Scholar]
  17. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586
    [Google Scholar]
  18. Moore W. E. C., Moore L. U. H. 1986; Genus Eubacterium . In Bergey's Manual of Systematic Bacteriology vol. 2 pp  1353–1373 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  19. Moore W. E. C., Holdeman L. V., Kelley R. W. 1984; Genus Fusobacterium . In Bergey's Manual of Systematic Bacteriology vol 1 pp  631–637 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  20. Nandi R., Sengupta S. 1998; Microbial production of hydrogen: an overview. Crit Rev Microbiol 24:61–84 [CrossRef]
    [Google Scholar]
  21. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212 [CrossRef]
    [Google Scholar]
  22. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . Technical Note 101: Newark, DE: MIDI;
    [Google Scholar]
  23. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  25. Winker S., Woese C. R. 1991; A definition of the domains Archaea , Bacteria and Eucarya in terms of small subunit ribosomal RNA characteristics. Syst Appl Microbiol 14:305–310 [CrossRef]
    [Google Scholar]
  26. Zellner G., Stackebrandt E., Nagel D., Messner P., Weiss N., Winter J. 1996; Anaerofilum pentosovorans gen. nov., sp. nov., and Anaerofilum agile sp. nov., two new, strictly anaerobic, mesophilic, acidogenic bacteria from anaerobic bioreactors. Int J Syst Bacteriol 46:871–875 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63212-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63212-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error