1887

Abstract

A polyphasic study was carried out to clarify the taxonomic position of two Gram-positive bacteria isolated from soil samples of the Grotta dei Cervi (Italy), a relatively unexplored hypogean environment. The strains, 20-5 and 23-23, showed phenotypic and phylogenetic characteristics that were consistent with their classification in the genus . 16S rRNA gene sequence comparisons revealed that the two strains formed distinct phyletic lines within the genus . Based on 16S rRNA gene sequence similarity, chemotaxonomic data and the results of DNA–DNA relatedness studies, it is proposed that the two isolates represent two novel species of the genus . Pronounced differences in a broad range of phenotypic characteristics and DNA G+C content distinguished the two strains from each other and from previously described species of the genus . Two novel species are proposed: sp. nov. (type strain, 20-5=HKI 0320=DSM 16198=NCIMB 13990) and sp. nov. (type strain, 23-23=HKI 0321=DSM 16197=NCIMB 13989).

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2005-01-01
2019-12-11
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