1887

Abstract

Two bacterial strains, F23 and R22, have been isolated from hypersaline habitats in Málaga (S. Spain) and Murcia (E. Spain). The novel strains, similar to previously described species, are slightly curved rods, Gram-negative, chemo-organotrophic, strictly aerobic and motile by a single polar flagellum. Both strains produce catalase and oxidase. They hydrolyse aesculin, gelatin, casein, Tween 20, Tween 80 and DNA but not starch or tyrosine. The strains differ from the hitherto described species in their capacity to produce extracellular polysaccharides and their different patterns of carbon sources and antimicrobial susceptibility. They are moderate halophiles capable of growing in NaCl concentrations of 0·5 to 25 % w/v, the optimum being 3–5 % w/v. Cellular fatty acids are predominantly iso-branched. The main fatty acids in strain FP23 are 15 : 0 iso (26·75 %), 16 : 17 (11·33 %) and 16 : 0 (11·73 %) whilst 15 : 0 iso (24·69 %), 17 : 0 iso (12·92 %) and 17 : 19 (11·03 %) are predominant in strain R22. The DNA G+C composition is 46·0 mol% in strain FP23 and 48·7 mol% in strain R22. Phylogenetic analyses indicate conclusively that the two strains belong to the genus . DNA–DNA hybridization revealed that they represent novel species. In the light of the polyphasic evidence accumulated in this study, it is proposed that they be classified as novel species of the genus , with the names sp. nov. (type strain F23=CECT 5859=LMG 22169) and sp. nov. (type strain R22=CECT 5858=LMG 22170).

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2004-09-01
2019-12-13
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