1887

Abstract

Two Gram-positive bacterial strains, CM2104 and CM2110, isolated from the inner part of abnormally spoiled oriental melon () in Korea, were subjected to a polyphasic taxonomic study. The cell-wall peptidoglycan of strains CM2104 and CM2110 contained -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-8(H). The major fatty acids detected in the two strains were iso-C, C 8 and C 9 or C. The DNA G+C content of the two strains was 73 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strains formed a coherent cluster with a clade comprising two species, and . Strains CM2104 and CM2110 exhibited a 16S rRNA gene sequence similarity value of 99·7 % and a mean DNA–DNA relatedness level of 89 %. Strains CM2104 and CM2110 showed 16S rRNA gene sequence similarity levels of 97·8–98·4 % to the type strains of and . DNA–DNA relatedness between strains CM2104 and CM2110 and the type strains of these two species was 7–11 %. On the basis of the phenotypic and phylogenetic data and genomic distinctiveness, strains CM2104 and CM2110 should be placed within the genus as members of a novel species, for which the name sp. nov. is proposed. The type strain is CM2104 (=KCTC 9987=DSM 16063=JCM 12321).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63167-0
2004-11-01
2021-03-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs541975.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63167-0&mimeType=html&fmt=ahah

References

  1. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1993 phylip: Phylogenetic Inference Package, version 3.5. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  5. Gordon R. E., Mihm J. M. 1957; A comparative study of some strains received as nocardiae. J Bacteriol 73:15–27
    [Google Scholar]
  6. Groth I., Schumann P., Martin K., Schuetze B., Augsten K., Kramer I., Stackebrandt E. 1999; Ornithinicoccus hortensis gen. nov., sp. nov., a soil actinomycete which contains l-ornithine. Int J Syst Bacteriol 49:1717–1724 [CrossRef]
    [Google Scholar]
  7. Groth I., Schumann P., Schütze B., Augsten K., Stackebrandt E. 2002; Knoellia sinensis gen. nov., sp. nov. and Knoellia subterranea sp. nov., two novel actinobacteria isolated from a cave. Int J Syst Evol Microbiol 52:77–84
    [Google Scholar]
  8. Hanada S., Liu W.-T., Shintani T., Kamagata Y., Nakamura K. 2002; Tetrasphaera elongata sp. nov., a polyphosphate-accumulating bacterium isolated from activated sludge. Int J Syst Evol Microbiol 52:883–887 [CrossRef]
    [Google Scholar]
  9. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria. J Bacteriol 66:24–26
    [Google Scholar]
  10. Imamura Y., Ikeda M., Yoshida S., Kuraishi H. 2000; Janibacter brevis sp. nov., a new trichloroethylene-degrading bacterium isolated from polluted environments. Int J Syst Evol Microbiol 50:1899–1904
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  12. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  13. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
    [Google Scholar]
  14. Lang E., Kroppenstedt R. M., Swiderski J., Schumann P., Ludwig W., Schmid A., Weiss N. 2003; Emended description of Janibacter terrae , including ten dibenzofuran-degrading strains and Janibacter brevis as its later heterotypic synonym. Int J Syst Evol Microbiol 53:1999–2005 [CrossRef]
    [Google Scholar]
  15. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  16. Martin K., Schumann P., Rainey F. A., Schuetze B., Groth I. 1997; Janibacter limosus gen. nov., sp. nov. a new actinomycete with meso -diaminopimelic acid in the cell wall. Int J Syst Bacteriol 47:529–534 [CrossRef]
    [Google Scholar]
  17. Maszenan A. M., Seviour R. J., Patel B. K. C., Schumann P., Burghardt J., Tokiwa Y., Stratton H. M. 2000; Three isolates of novel polyphosphate-accumulating Gram-positive cocci, obtained from activated sludge, belong to a new genus, Tetrasphaera gen. nov., and description of two new species, Tetrasphaera japonica sp.nov. and Tetrasphaera australiensis sp. nov. Int J Syst Evol Microbiol 50:593–603 [CrossRef]
    [Google Scholar]
  18. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  20. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI Inc;
    [Google Scholar]
  21. Stackebrandt E., Schumann P. 2000; Description of Bogoriellaceae fam.nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov.and emendation of some families of the suborder Micrococcineae . Int J Syst Evol Microbiol 50:1279–1285 [CrossRef]
    [Google Scholar]
  22. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  23. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  24. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  26. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  27. Yoon J.-H., Lee K.-C., Kang S.-S., Kho Y. H., Kang K. H., Park Y.-H. 2000; Janibacter terrae sp. nov., a bacterium isolated from soil around a wastewater treatment plant. Int J Syst Evol Microbiol 50:1821–1827
    [Google Scholar]
  28. Yoon J.-H., Kim I.-G., Kang K. H., Oh T.-K., Park Y.-H. 2003; Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 53:1297–1303 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63167-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63167-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error