1887

Abstract

A polyphasic study was undertaken to establish the taxonomic status of a soil isolate. The organism, strain FXJ14, was found to have chemical and morphological properties characteristic of streptomycetes. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence of the strain and on the 120 nt variable -region of the 16S rRNA molecule showed that it formed a distinct phyletic line within the range of variation encompassed by the genus . The sharp separation of the organism from representatives of the genus was strengthened by the fact that its BOX-PCR and RFLP of 16S rDNA-ITS fingerprints differed from those of over 450 recognized species. The isolate also had a profile of phenotypic properties that readily distinguished it from the genotypically close type strains. It is evident from the combination of genotypic and phenotypic data that strain FXJ14 (=AS 4.1858=JCM 12278=LMG 22082) should be classified as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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2004-11-01
2019-12-14
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vol. , part 6, pp. 2085 - 2089

Scanning electron micrograph showing spore chain morphology and spore surface ornamentation of strain FXJ14 grown on Bennett’s agar for 14 days at 28 °C. Scale bar, 5.0 µm.

Partial neighbour-joining tree based on the 120 nt gamma-region of the 16S rRNA gene showing the position of strain FXJ14 in the tree. Numbers at nodes indicate levels of bootstrap support (%) based on a neighbour-joining analysis of 1000 resampled data sets; only values above 50 % are given. The scale bar corresponds to 0.02 substitutions per nucleotide position.

Partial dendrogram of UPGMA cluster analysis of RFLP of 16S-ITS fingerprints of strain FXJ14 and type strains.

Partial dendrogram of UPGMA cluster analysis of BOX-PCR fingerprints of strain FXJ14 and type strains.

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