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Abstract

A novel alkaliphile was isolated from a drain of a fish processing plant. The isolate grew at a pH range of 7–10. Cells were Gram-positive, facultatively aerobic, motile rods with peritrichous flagella. Colonies were orange or yellow in colour. Catalase and oxidase reactions were positive. The isolate grew in 0–12 % NaCl but not above 15 % NaCl. Its cell extract exhibited 567 times higher catalase activity than an cell extract. The major cellular fatty acids were iso-C, anteiso-C, iso-C, iso-C, iso-C, anteiso-C and iso-C. Its DNA G+C content was 46·7 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing and chemotaxonomic data indicated that strain T-2-2 is a member of the genus . DNA–DNA hybridization revealed a low relatedness of the isolate to several phylogenetic neighbours (less than 25 %). On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA relatedness data, the isolate merits classification as a novel species, for which the name sp. nov. is proposed. The type strain is T-2-2 (=JCM 12280=NCIMB 13980).

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2004-11-01
2019-10-19
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vol. , part 6, pp. 2013–2017

Details of the catalase activity of strain T-2-2 and reference strains are available to download. [PDF](116KB)



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