1887

Abstract

Four strains of cold-adapted, strictly aerobic and facultative oligotrophic bacteria were isolated from polar seas and investigated using a polyphasic taxonomic approach. Two strains (LMG 21857 and LMG 21854) derive from Arctic sea water whereas the other two strains (LMG 21855 and LMG 21858) were isolated from Antarctic sea water. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belong to the -subclass of the and are related to the genus , with 98·0–99·7 % sequence similarity to and 94·2–95·3 % sequence similarity to , their nearest phylogenetic neighbours. Two strains (LMG 21855 and LMG 21858) were identified as , whereas DNA–DNA hybridization results and differences in phenotypic characteristics showed that the other two strains (LMG 21857 and LMG 21854) constitute a novel species within the genus , with a DNA G+C content of 44·0 mol%. The isolates are Gram-negative, chemoheterotrophic, motile, rod-shaped cells that are psychrotolerant and moderately halophilic. Buds can be produced on mother cells and on prosthecae. Branch formation of prosthecae occurs. Whole-cell fatty acid profiles of the isolates are very similar and include C and C 7 as the major fatty acid components. On the basis of genotypic and phenotypic properties, a novel species of the genus is described, for which the name sp. nov. is proposed, with isolate LMG 21857 (=CIP 108324=ARK 150) as the type strain. An emended description of the genus is presented.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63123-0
2004-09-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/5/ijs541765.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63123-0&mimeType=html&fmt=ahah

References

  1. Bowman, J. P., McCammon, S. A., Brown, J. L. & McMeekin, T. A. ( 1998; ). Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov. psychrophilic bacteria from Antarctic sea-ice habitats. Int J Syst Bacteriol 48, 1213–1222.[CrossRef]
    [Google Scholar]
  2. Coenye, T., Falsen, E., Vancanneyt, M., Hoste, B., Govan, J. R., Kersters, K. & Vandamme, P. ( 1999; ). Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49, 405–413.[CrossRef]
    [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Ivanova, E. P., Zhukova, N. V., Svetashev, V. I., Gorshkova, N. M., Kurilenko, V. V., Frolova, G. M. & Mikhailov, V. V. ( 2000; ). Evaluation of phospholipid and fatty acid compositions as chemotaxonomic markers of Alteromonas-like proteobacteria. Curr Microbiol 41, 341–345.[CrossRef]
    [Google Scholar]
  5. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  6. Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Braker, G. & Hirsch, P. ( 1998; ). Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho Lake. Int J Syst Bacteriol 48, 1363–1372.[CrossRef]
    [Google Scholar]
  7. Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Schumann, P. & Hirsch, P. ( 1999; ). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49, 137–147.[CrossRef]
    [Google Scholar]
  8. Mergaert, J., Verhelst, A., Cnockaert, M. C., Tan, T.-L. & Swings, J. ( 2001; ). Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences. Syst Appl Microbiol 24, 98–107.[CrossRef]
    [Google Scholar]
  9. Mikhailov, V. V., Romanenko, L. A. & Ivanova, E. P. ( 2002; ). The genus Alteromonas and related Proteobacteria. In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community. Edited by M. Dworkin, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  10. Pitcher, D. G., Saunders, N. A. & Owen, R. J. ( 1989; ). Rapid extraction of bacterial genomic DNA with guanidinium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef]
    [Google Scholar]
  11. Rademaker, J. L. W. & de Bruijn, F. J. ( 1997; ). Characterization and classification of microbes by rep-PCR genomic fingerprinting and computer assisted pattern analysis. In DNA Markers: Protocols, Applications and Overviews, pp. 151–171. Edited by G. Caetano-Anollés & P. M. Gresshoff. New York: Wiley.
  12. Rademaker, J. L. W., Hoste, B., Louws, F. J., Kersters, K., Swings, J., Vauterin, L., Vauterin, P. & de Bruijn, F. J. ( 2000; ). Comparison of AFLP and rep-PCR genomic fingerprinting with DNA–DNA homology studies: Xanthomonas as a model system. Int J Syst Evol Microbiol 50, 665–677.[CrossRef]
    [Google Scholar]
  13. Reichenbach, H. & Dworkin, M. ( 1981; ). Introduction to the gliding bacteria. In The Prokaryotes, vol. 1, pp. 315–327. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer.
  14. Romanenko, L. A., Zhukova, N. V., Rohde, M., Lysenko, A. M., Mikhailov, V. V. & Stackebrandt, E. ( 2003; ). Glaciecola mesophila sp. nov., a novel marine agar-digesting bacterium. Int J Syst Evol Microbiol 53, 647–651.[CrossRef]
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Microbiology, pp. 611–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  17. Tan, T. L. ( 1986; ). Construction and use of a dialysis chamber for investigating in situ the toxicity of heavy metals on bacteria. IFREMER Actes Colloq 3, 589–595.
    [Google Scholar]
  18. Tan, T. L. ( 1997; ). Biolog metabolic fingerprints for clustering marine oligotrophic bacteria from polar regions. In Microbial Communities – Functional versus Structural Approaches, pp. 161–170. Edited by H. Insam & A. Rangger. Berlin: Springer.
  19. Tan, T. L. & Rüger, H. J. ( 1991; ). Biomass and nutritional requirements of psychrotrophic bacterial communities in Fram Strait and Western Greenland Sea. Kiel Meeresforsch Sonderh 8, 219–224.
    [Google Scholar]
  20. Tan, T. L. & Rüger, H. J. ( 1999; ). Enrichment, isolation, and Biolog metabolic fingerprints of oligotrophic bacteria from the Antarctic Ocean. Arch Hydrobiol Spec Issues Adv Limnol 54, 255–272.
    [Google Scholar]
  21. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
  22. van Gemerden, H. & Kuenen, J. G. ( 1984; ). Strategies for growth and evolution of microorganisms in oligotrophic habitats. In Heterotrophic Activity in the Sea (Nato Conference Series IV. Marine Sciences), vol. 15, pp. 25–54. Edited by J. E. Hobbie & P. J. Williams. New York: Plenum.
  23. Van Trappen, S., Mergaert, J. & Swings, J. ( 2003; ). Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53, 1241–1245.[CrossRef]
    [Google Scholar]
  24. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. ( 2004a; ). Flavobacterium degerlachei sp. nov., Flavobacterium frigoris sp. nov. and Flavobacterium micromati sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54, 85–92.[CrossRef]
    [Google Scholar]
  25. Van Trappen, S., Tan, T.-L., Yang, J., Mergaert, J. & Swings, J. ( 2004b; ). Alteromonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus Alteromonas. Int J Syst Evol Microbiol 54, 1157–1163.[CrossRef]
    [Google Scholar]
  26. Versalovic, J., Koeuth, T. & Lupski, J. R. ( 1991; ). Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting in bacterial genomes. Nucleic Acids Res 19, 6823–6831.[CrossRef]
    [Google Scholar]
  27. Versalovic, J., Schneider, M., de Bruijn, F. J. & Lupski, J. R. ( 1994; ). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5, 25–40.
    [Google Scholar]
  28. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  29. Weiner, R. M., Melick, M., O'Neill, K. & Quintero, E. ( 2000; ). Hyphomonas adhaerens sp. nov., Hyphomonas johnsonii sp. nov. and Hyphomonas rosenbergii sp. nov., marine budding and prosthecate bacteria. Int J Syst Evol Microbiol 50, 459–469.[CrossRef]
    [Google Scholar]
  30. West, P. A. & Colwell, R. R. ( 1984; ). Identification and classification of the Vibrionaceae – an overview. In Vibrios in the Environment, pp. 285–363. Edited by R. R. Colwell. New York: Wiley.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63123-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63123-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 1765 - 1771

Digitized representation of normalized rep-PCR profiles (GTG primer) and dendrogram derived from UPGMA clustering of the profiles with Pearson's correlation coefficient. The correlation coefficient is expressed as percentage similarity. [PDF](29 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error