1887

Abstract

Strain LB400 is the best-studied polychlorinated biphenyl (PCB) degrader. This organism has previously been allocated in the genus , since its 16S rRNA gene sequence shows 98·6 % sequence similarity to the type strains of and . A polyphasic study was undertaken to clarify the actual taxonomic position of this biotechnologically important organism and of two strains, one recovered from a blood culture vial and one from a coffee plant rhizosphere, both of which resembled strain LB400 in their whole-cell protein patterns. DNA–DNA hybridization experiments revealed that the three strains represented a single novel species, for which the name sp. nov. is proposed. Strains of this novel species can be differentiated phenotypically from nearly all other species by their inability to assimilate -arabinose. The whole-cell fatty acid profile of strains is consistent with their classification in the genus , with 18 : 17, 16 : 17, 16 : 0, 14 : 0 3OH, 16 : 0 3OH, 17 : 0 cyclo and 14 : 0 being the most abundant fatty acids. The G+C content of the species varies between 62·4 and 62·9 mol%. The type strain of is LB400 (=LMG 21463=CCUG 46959=NRRL B-18064).

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2004-09-01
2019-09-16
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References

  1. Achouak, W., Christen, R., Barakat, M., Martel, M.-H. & Heulin, T. ( 1999; ). Burkholderia caribensis sp. nov., an exopolysaccharide-producing bacterium isolated from vertisol microaggregates in Martinique. Int J Syst Bacteriol 49, 787–794.[CrossRef]
    [Google Scholar]
  2. Bartels, F., Backhaus, S., Moore, E. R. B., Timmis, K. N. & Hofer, B. ( 1999; ). Occurrence and expression of glutathiane-S-transferase-encoding bphK genes in Burkholderia sp. strain LB400 and other biphenyl-utilizing bacteria. Microbiology 145, 2821–2834.
    [Google Scholar]
  3. Bedard, D. L., Unterman, R., Bopp, L. H., Brennan, M. J., Haberl, M. L. & Johnson, C. ( 1986; ). Rapid assay for screening and characterizing microorganisms for the ability to degrade polychlorinated biphenyls. Appl Environ Microbiol 51, 761–768.
    [Google Scholar]
  4. Bopp, L. H. ( 1986; ). Degradation of highly chlorinated PCBs by Pseudomonas strain LB400. J Ind Microbiol 1, 23–29.[CrossRef]
    [Google Scholar]
  5. Bopp, L. H. ( 1989; ). US patent # 4,843,009. Pseudomonas putida capable of degrading PCBs. June 27, 1989. Inventor: Lawrence H. Bopp, Scotia, NY Assignee: General Electric Company, Schenectady, NY.
  6. Brosius, J., Dull, T. J., Sleeter, D. D. & Noller, H. F. ( 1981; ). Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol 148, 107–127.[CrossRef]
    [Google Scholar]
  7. Coenye, T. & Vandamme, P. ( 2003; ). Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol 5, 719–729.[CrossRef]
    [Google Scholar]
  8. Coenye, T., Laevens, S., Willems, A., Ohlén, M., Hannant, W., Govan, J. R. W., Gillis, M., Falsen, E. & Vandamme, P. ( 2001; ). Burkholderia fungorum sp. nov., and Burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples. Int J Syst Evol Microbiol 51, 1099–1107.[CrossRef]
    [Google Scholar]
  9. Erickson, B. D. & Mondello, F. J. ( 1992; ). Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated biphenyl-degrading enzyme in Pseudomonas strain LB400. J Bacteriol 174, 2903–2912.
    [Google Scholar]
  10. Estrada-de los Santos, P., Bustillos-Cristales, R. & Caballero-Mellado, J. ( 2001; ). Burkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution. Appl Environ Microbiol 67, 2790–2798.[CrossRef]
    [Google Scholar]
  11. Fain, M. G. & Haddock, J. D. ( 2001; ). Phenotypic and phylogenetic characterization of Burkholderia (Pseudomonas) sp. strain LB400. Curr Microbiol 42, 269–275.
    [Google Scholar]
  12. Furukawa, K. ( 2000; ). Biochemical and genetic bases of microbial degradation of polychlorinated biphenyls (PCBs). J Gen Appl Microbiol 46, 283–296.[CrossRef]
    [Google Scholar]
  13. Gibson, D. T., Cruden, D. L., Haddock, J. D., Zylstra, G. J. & Brand, J. M. ( 1993; ). Oxidation of polychlorinated biphenyls by Pseudomonas sp. strain LB400 and Pseudomonas pseudoalcaligenes KF707. J Bacteriol 175, 4561–4564.
    [Google Scholar]
  14. Goris, J., De Vos, P., Coenye, T. & 7 other authors ( 2001; ). Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov., and Ralstonia basilensis Steinle et al. 1998 emend. Int J Syst Evol Microbiol 51, 1773–1782.[CrossRef]
    [Google Scholar]
  15. Goris, J., Dejonghe, W., Falsen, E., De Clerck, E., Geeraerts, B., Willems, A., Top, E. M., Vandamme, P. & De Vos, P. ( 2002; ). Diversity of transconjugants that acquired plasmid pJP4 or pEMT1 after inoculation of a donor strain in the A- and B-horizon of an agricultural soil and description of Burkholderia hospita sp. nov. and Burkholderia terricola sp. nov. Syst Appl Microbiol 25, 340–352.[CrossRef]
    [Google Scholar]
  16. Hofer, B., Eltis, L. D., Dowling, D. N. & Timmis, K. N. ( 1993; ). Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation. Gene 130, 47–55.[CrossRef]
    [Google Scholar]
  17. Kumamaru, T., Suenaga, H., Mitsuoka, M., Watanabe, T. & Furukawa, K. ( 1998; ). Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase. Nat Biotechnol 16, 663–666.[CrossRef]
    [Google Scholar]
  18. Maltseva, O. V., Tsoi, T. V., Quensen, J. F., III, Fukuda, M. & Tiedje, J. M. ( 1999; ). Degradation of anaerobic reductive dechlorination products of Aroclor 1242 by four aerobic bacteria. Biodegradation 10, 363–371.[CrossRef]
    [Google Scholar]
  19. Mascarua-Esparza, M. A., Villa-Gonzalez, R. & Caballero-Mellado, J. ( 1988; ). Acetylene reduction and indoleacetic acid production by Azospirillum isolates from cactaceous plants. Plant Soil 106, 91–95.[CrossRef]
    [Google Scholar]
  20. Mueller, J. G., Devereux, R., Santavy, D. L., Lantz, S. E., Willis, S. G. & Pritchard, P. H. ( 1997; ). Phylogenetic and physiological comparisons of PAH-degrading bacteria from geographically diverse soils. Antonie Van Leeuwenhoek 71, 329–343.[CrossRef]
    [Google Scholar]
  21. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  22. Rademaker, J. L. W. & De Bruijn, F. J. ( 1997; ). Characterization and classification of microbes by rep-PCR genomic fingerprinting and computer assisted pattern analysis. In DNA Markers: Protocols, Applications and Overviews, pp. 151–171. Edited by G. Caetano-Anollés & P. M. Gresshoff. New York: Wiley.
  23. Seeger, M., Zielinski, M., Timmis, K. N. & Hofer, B. ( 1999; ). Regiospecificity of dioxygenation of di- to pentachlorobiphenyls and their degradation to chlorobenzoates by the bph-encoded catabolic pathway of Burkholderia sp. strain LB400. Appl Environ Microbiol 65, 3614–3621.
    [Google Scholar]
  24. Stackebrandt, E., Frederiksen, W., Garrity, G. M. & 10 other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  25. Stead, D. E. ( 1992; ). Grouping of plant-pathogenic and some other Pseudomonas spp. by using cellular fatty acid profiles. Int J Syst Bacteriol 42, 281–295.[CrossRef]
    [Google Scholar]
  26. Vandamme, P., Gillis, M., Vancanneyt, M., Hoste, B., Kersters, K. & Falsen, E. ( 1993; ). Moraxella lincolnii sp. nov., isolated from the human respiratory tract, and reevaluation of the taxonomic position of Moraxella osloensis. Int J Syst Bacteriol 43, 474–481.[CrossRef]
    [Google Scholar]
  27. Vandamme, P., Goris, J., Chen, W.-M., De Vos, P. & Willems, A. ( 2002; ). Burkholderia tuberum sp. nov. and Burkholderia phymatum sp. nov., nodulate the roots of tropical legumes. Syst Appl Microbiol 25, 507–512.[CrossRef]
    [Google Scholar]
  28. Vauterin, L., Yang, P., Hoste, B., Vancanneyt, M., Civerolo, E. L., Swings, J. & Kersters, K. ( 1991; ). Differentiation of Xanthomonas campestris pv. citri strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins, fatty acid analysis, and DNA-DNA hybridization. Int J Syst Bacteriol 41, 535–542.[CrossRef]
    [Google Scholar]
  29. Viallard, V., Poirier, I., Cournoyer, B., Haurat, J., Wiebkin, S., Ophel-Keller, K. & Balandreau, J. ( 1998; ). Burkholderia graminis sp. nov., a rhizospheric Burkholderia species, and reassessment of [Pseudomonas] phenazinium, [Pseudomonas] pyrrocinia and [Pseudomonas] glathei as Burkholderia. Int J Syst Bacteriol 48, 549–563.[CrossRef]
    [Google Scholar]
  30. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  31. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
  32. Zaitsev, G. M. & Karasevich, Y. N. ( 1985; ). Primary steps in metabolism of 4-chlorobenzoate in Arthrobacter globiformis. Mikrobiologiya 50, 423–428 (in Russian).
    [Google Scholar]
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vol. , part 5, pp. 1677–1681

BOX-PCR patterns and ribotype profiles are available to download. [PDF](176KB)



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