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Abstract

An actinomycete strain (CB1190) was previously isolated from industrial sludge contaminated with 1,4-dioxane. The cells of this culture are Gram-positive and exhibit branching aerial and vegetative mycelium. Analysis of the 16S rRNA gene sequence indicates that the strain belongs to the genus , closely related to , and . Physiological and biochemical characteristics of CB1190 are different from those of other known species. The novel organism described here is distinguished by its ability to grow on 1,4-dioxane, which is a probable human carcinogen. This culture can also grow on tetrahydrofuran, gasoline aromatics and several other toxic environmental contaminants. Strain CB1190 is capable of fixing dinitrogen. The predominant fatty acids are 16 : 0 iso, 16 : 1 iso 9 and 17 : 1 iso 9. The major phospholipid fatty acids are 16 : 0 iso, 16 : 0 10-Me and 17 : 0 10-Me. The peptidoglycan belongs to type A1, -diaminopimelic acid. The major menaquinone is MK-8 (H). Mycolic acids are absent. The G+C content is 74 mol%. Based on morphological, physiological, chemotaxonomic and phylogenetic evidence, it is proposed that strain CB1190 (=ATCC 55486=DSM 44775) be classified as the type strain of a novel species, sp. nov. Further studies with this organism will provide insights into metabolic pathways, responsible enzymes, kinetics and the fate of 1,4-dioxane in the environment.

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2005-03-01
2019-10-14
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Cellular fatty acid compositions of strain CB1190 , and DSM 43281 . [PDF](15 KB)

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Scanning electron micrograph of 14-day-old cells of CB1190 grown at 30 °C on agar plates made with AMS medium and 1,4-dioxane. Bar, 1 µm.

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