1887

Abstract

A previously undescribed, Gram-positive, catalase-negative, coccus-shaped organism that originated from a human wound was subjected to taxonomic study. On the basis of its cellular morphology and the results of biochemical testing, the unknown organism was identified tentatively as a member of the genus , but it did not correspond to either of the two recognized species of this genus. Comparative 16S rRNA gene sequencing studies confirmed that the bacterium was associated phylogenetically with the genus , with the unidentified organism forming a hitherto unknown subline within the genus. On the basis of biochemical, molecular chemical and molecular phylogenetic evidence, it is proposed that the unknown organism that was recovered from a human wound should be classified as a novel species of the genus , namely sp. nov. The type strain is CCUG 47334 (=CIP 108183).

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2004-09-01
2020-01-27
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References

  1. Caliendo, A. M., Jordan, C. D. & Ruoff, K. L. ( 1995; ). Helcococcus, a new genus of catalase-negative, gram-positive cocci isolated from clinical specimens. J Clin Microbiol 33, 1638–1639.
    [Google Scholar]
  2. Collins, M. D., Facklam, R. R., Rodrigues, U. M. & Ruoff, K. L. ( 1993; ). Phylogenetic analysis of some Aerococcus-like organisms from clinical sources: description of Helcococcus kunzii gen. nov., sp. nov. Int J Syst Bacteriol 43, 425–429.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Falsen, E., Foster, G., Monasterio, L. R., Dominguez, L. & Fernandez-Garazabal, J. F. ( 1999; ). Helcococcus ovis sp. nov., a Gram-positive organism from sheep. Int J Syst Bacteriol 49, 1429–1432.[CrossRef]
    [Google Scholar]
  4. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. (editors) ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  5. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  6. Nicholas, K. B., Nicholas, H. B., Jr & Deerfield, D. W., II ( 1997; ). GeneDoc: analysis and visualization of genetic variation. EMBNEW News 4, 14.
    [Google Scholar]
  7. Page, R. D. M. ( 1996; ). treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  8. Peel, M. M., Davis, J. M., Griffin, K. J. & Freedman, D. L. ( 1997; ). Helcococcus kunzii as sole isolate from an infected sebaceous cyst. J Clin Microbiol 35, 328–329.
    [Google Scholar]
  9. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  10. Rasmussen, S. W. ( 1995; ). DNATools, a software package for DNA sequence analysis. Carlsberg Laboratory, Copenhagen.
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