1887

Abstract

A strictly anaerobic arsenate-respiring bacterium isolated from a gold mine in Bendigo, Victoria, Australia, belonging to the genus is described. Cells are Gram-positive, motile rods capable of respiring with arsenate and nitrate as terminal electron acceptors using a variety of substrates, including acetate as the electron donor. Reduction of arsenate to arsenite is catalysed by a membrane-bound arsenate reductase that displays activity over a broad pH range. Synthesis of the enzyme is regulated; maximal activity is obtained when the organism is grown with arsenate as the terminal electron acceptor and no activity is detectable when it is grown with nitrate. Mass of the catalytic subunit was determined to be approximately 87 kDa based on ingel activity stains. The closest phylogenetic relative, based on 16S rRNA gene sequence analysis, is , but DNA–DNA hybridization experiments clearly show that strain JMM-4 represents a novel species, for which the name sp. nov. is proposed. The type strain is JMM-4 (=DSM 16346=JCM 12340).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63059-0
2004-11-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs542241.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63059-0&mimeType=html&fmt=ahah

References

  1. Afkar E., Lisak J., Saltikov C., Basu P., Oremland R. S., Stolz J. F. 2003; The respiratory arsenate reductase from Bacillus selenitireducens strain MLS10. FEMS Microbiol Lett 226:107–112 [CrossRef]
    [Google Scholar]
  2. Bluemle S., Zumft W. G. 1991; Respiratory nitrate reductase from denitrifying Pseudomonas stutzeri , purification, properties and target of proteolysis. Biochim Biophys Acta 1057:102–108 [CrossRef]
    [Google Scholar]
  3. Cashion P., Hodler-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  5. Escara J. F., Hutton J. R. 1980; Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of renaturation rate. Biopolymers 19:1315–1327 [CrossRef]
    [Google Scholar]
  6. Huss V. A. R., Festel H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  7. Krafft T., Macy J. M. 1998; Purification and characterization of the respiratory arsenate reductase of Chrysiogenes arsenatis . Eur J Biochem 255:647–653 [CrossRef]
    [Google Scholar]
  8. Oremland R. S., Stolz J. F. 2003; The ecology of arsenic. Science 300:939–944 [CrossRef]
    [Google Scholar]
  9. Saltikov C. W., Newman D. K. 2003; Genetic identification of a respiratory arsenate reductase. Proc Natl Acad Sci U S A 100:10983–10988 [CrossRef]
    [Google Scholar]
  10. Santini J. M., Stolz J. F., Macy J. M. 2002; Isolation of a new arsenate-respiring bacterium – physiological and phylogenetic studies. Geomicrobiol J 19:41–52 [CrossRef]
    [Google Scholar]
  11. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  12. Stackebrandt E., Frederiksen W., Garrity G. M. 10 other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  13. Switzer Blum J., Burns Bindi A., Buzzelli J., Stolz J. F., Oremland R. S. 1998; Bacillus arsenicoselenatis , sp. nov., and Bacillus selenitireducens , sp. nov.: two haloalkaliphiles from Mono Lake, California that respire oxyanions of selenium and arsenic. Arch Microbiol 171:19–30 [CrossRef]
    [Google Scholar]
  14. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.63059-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63059-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error