1887

Abstract

A bacterial strain, designated B13, was isolated from spoiled red wine from the Dão region, Portugal. The strain was Gram-negative, strictly aerobic, rod-shaped and motile. Phylogenetic analysis on the basis of 16S rRNA gene sequences indicated that B13 belonged to the genus within the . The closest related species was , with 98·4 % 16S rRNA gene sequence similarity. DNA–DNA hybridization showed that B13 constituted a taxon separate from the species with validly published names. The DNA G+C content of B13 was 58·1 mol%. Phenotypic characteristics of B13 allowed its differentiation from the recognized species. B13 produced 5-ketogluconic acid from glucose, but no 2-ketogluconic acid. It produced catalase, but no oxidase. It utilized glycerol, but not maltose, ethanol or methanol as carbon sources. On the basis of the results obtained, B13 represents a novel species for which the name sp. nov. is proposed. The type strain is B13 (=LMG 21952=CECT 5830).

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2006-01-01
2019-12-12
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