1887

Abstract

The taxonomic position of a group of actinobacterial strains isolated from the phyllosphere of potato plants was investigated by using a polyphasic approach. Although the similarity values for their 16S rRNA gene sequences suggested an intermediate position between and , the phylogenetic tree demonstrated a clear clustering of the representative strain, K 114/01, within the genus . The presence of 2,4-diaminobutyric acid as the diagnostic diamino acid in the cell-wall peptidoglycan of strain K 114/01 substantiated the affiliation to the genus . An analysis performed using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry revealed highly similar spectral patterns for the isolated strains, which, together with their conformity regarding a multitude of phenotypic features, supported their affiliation to the same species. Differences in several physiological features, peptidoglycan and menaquinone composition and whole-cell fatty acid profiles enabled discrimination of the phyllosphere isolates with respect to recognized species. As the 16S rRNA gene sequence similarity values were below 97 %, the strains isolated from the phyllosphere of potato plants represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K 114/01 (=DSM 19812 =LMG 24386).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.2008/001610-0
2008-12-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/12/2833.html?itemId=/content/journal/ijsem/10.1099/ijs.0.2008/001610-0&mimeType=html&fmt=ahah

References

  1. Behrendt, U., Müller, T. & Seyfarth, W.(1997). The influence of extensification in grassland management on the populations of micro-organisms in the phyllosphere of grasses. Microbiol Res 152, 75–85.[CrossRef] [Google Scholar]
  2. Behrendt, U., Ulrich, A., Schumann, P., Erler, W., Burghardt, J. & Seyfarth, W.(1999). A taxonomic study of bacteria isolated from grasses: a proposed new species Pseudomonas graminis sp. nov. Int J Syst Bacteriol 49, 297–308.[CrossRef] [Google Scholar]
  3. Behrendt, U., Ulrich, A. & Schumann, P.(2003). Fluorescent pseudomonads associated with the phyllosphere of grasses; Pseudomonas trivialis sp. nov., Pseudomonas poae sp. nov. and Pseudomonas congelans sp. nov. Int J Syst Evol Microbiol 53, 1461–1469.[CrossRef] [Google Scholar]
  4. Bora, N., Vancanneyt, M., Gelsomino, R., Swings, J., Brennan, N., Cogan, T. M., Larpin, S., Desmasures, N., Lechner, F. E. & other authors(2007).Agrococcus casei sp. nov., isolated from the surfaces of smear-ripened cheeses. Int J Syst Evol Microbiol 57, 92–97.[CrossRef] [Google Scholar]
  5. Conway, G. C., Smole, S. C., Sarracino, D. A., Arbeit, R. D. & Leopold, P. E.(2001). Phyloproteomics: species identification of Enterobacteriaceae using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Mol Microbiol Biotechnol 3, 103–112. [Google Scholar]
  6. Evtushenko, L. I. & Takeuchi, M.(2006). The family Microbacteriaceae. In The Prokaryotes; a Handbook on the Biology of Bacteria, 3rd edn, vol. 3, pp. 1020–1098. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  7. Felsenstein, J.(1981). Evolutionary tree from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  8. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Fenselau, C. & Demirev, P. A.(2001). Characterization of intact microorganisms by Maldi mass spectrometry. Mass Spectrom Rev 20, 157–171.[CrossRef] [Google Scholar]
  10. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef] [Google Scholar]
  11. Heuer, H. & Smalla, K.(1999). Bacterial phyllosphere communities of Solanum tuberosum L. and T4-lysozyme-producing transgenic variants. FEMS Microbiol Ecol 28, 357–371.[CrossRef] [Google Scholar]
  12. Katayama, T., Tanaka, M., Moriizumi, J., Nakamura, T., Brouchkov, A., Douglas, T. A., Fukuda, M., Tomita, F. & Asano, K.(2007). Phylogenetic analysis of bacteria preserved in a permafrost ice wedge for 25,000 years. Appl Environ Microbiol 73, 2360–2363.[CrossRef] [Google Scholar]
  13. La Duc, M. T., Dekas, A., Osman, S., Moissl, C., Newcombe, D. & Venkateswaran, K.(2007). Isolation and characterization of bacteria capable of tolerating the extreme conditions of clean room environments. Appl Environ Microbiol 73, 2600–2611.[CrossRef] [Google Scholar]
  14. Mayilraj, S., Suresh, K., Schumann, P., Kroppenstedt, R. M. & Saini, H. S.(2006).Agrococcus lahaulensis sp. nov., isolated from a cold desert of the Indian Himalayas. Int J Syst Evol Microbiol 56, 1807–1810.[CrossRef] [Google Scholar]
  15. Richard, C. & Kiredjian, M.(1995).Laboratory Methods for the Identification of Strictly Aerobic Gram-Negative Bacilli. Paris: Institut Pasteur.
  16. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  17. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  18. Stackebrandt, E., Päuker, O. & Erhard, M.(2005). Grouping myxococci (Corallococcus) strains by matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) mass spectrometry: comparison with gene sequence phylogenies. Curr Microbiol 50, 71–77.[CrossRef] [Google Scholar]
  19. Stackebrandt, E., Brambilla, E. & Richert, K.(2007). Gene sequence phylogenies of the family Microbacteriaceae. Curr Microbiol 55, 42–46.[CrossRef] [Google Scholar]
  20. Tiago, I., Chung, A. P. & Verissimo, A.(2004). Bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations. Appl Environ Microbiol 70, 7378–7387.[CrossRef] [Google Scholar]
  21. Tóth, E. M., Schumann, P., Borsodi, A. K., Kéki, Z., Kovács, A. L. & Márialigeti, K.(2008).Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from Wohlfahrtia magnifica (Diptera: Sarcophagidae). Int J Syst Evol Microbiol 58, 976–981.[CrossRef] [Google Scholar]
  22. Ulrich, K., Ulrich, A. & Ewald, D.(2008). Diversity of endophytic bacterial communities in poplar grown under field conditions. FEMS Microbiol Ecol 63, 169–180.[CrossRef] [Google Scholar]
  23. Valentine, N., Wunschel, S., Wunschel, D., Petersen, C. & Wahl, K.(2005). Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry. Appl Environ Microbiol 71, 58–64.[CrossRef] [Google Scholar]
  24. Wieser, M., Schumann, P., Martin, K., Altenburger, P., Burghardt, J., Lubitz, W. & Busse, H. J.(1999).Agrococcus citreus sp. nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria). Int J Syst Bacteriol 49, 1165–1170.[CrossRef] [Google Scholar]
  25. Zhang, G., Niu, F., Ma, X., Liu, W., Dong, M., Feng, H., An, L. & Cheng, G.(2007). Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region. Can J Microbiol 53, 1000–1010.[CrossRef] [Google Scholar]
  26. Zlamala, C., Schumann, P., Kämpfer, P., Rosselló-Mora, R., Lubitz, W. & Busse, H.-J.(2002).Agrococcus baldri sp. nov., isolated from the air in the ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol 52, 1211–1216.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.2008/001610-0
Loading
/content/journal/ijsem/10.1099/ijs.0.2008/001610-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error