1887

Abstract

A Gram-positive, short, rod-shaped, cream-coloured actinobacterium, designated MSL-04, was isolated from a soil sample collected from an agricultural field on Bigeum Island (Republic of Korea) and was subjected to polyphasic characterization to unravel its taxonomic position. The optimum growth temperature and pH were 28–30 °C and pH 7.2–7.4. Chemotaxonomic characteristics such as B2-type peptidoglycan with glycolyl residues, MK-11 and MK-12 as the major menaquinones and a fatty acid profile containing iso- and anteiso-branched fatty acids placed this organism within the genus . Phylogenetic analysis based on 16S rRNA gene sequences confirmed the position of strain MSL-04 in the genus and showed that it belonged to the same clade as but was distinct from its nearest neighbours. The 16S rRNA gene sequence of strain MSL-04 showed the highest levels of similarity with respect to LMG 20991 (97.5 % similarity) and IFO 3750 (97 %). The G+C content of the DNA was 71.0 mol%. The DNA–DNA relatedness between MSL-04 and its closest neighbours (<70 %) showed that the strain represents a distinct genomic species. On the basis of the phenotypic and genotypic results, strain MSL-04 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is MSL-04 (=KCTC 19269 =DSM 19265).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.2008/001222-0
2008-11-01
2020-12-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/11/2536.html?itemId=/content/journal/ijsem/10.1099/ijs.0.2008/001222-0&mimeType=html&fmt=ahah

References

  1. Chen, H.-H., Li, W.-J., Tang, S.-K., Kroppenstedt, R. M., Stackebrandt, E., Xu, L.-H. & Jiang, C.-L. ( 2004; ). Corynebacterium halotolerans sp. nov., isolated from saline soil in the west of China. Int J Syst Evol Microbiol 54, 779–782.[CrossRef]
    [Google Scholar]
  2. Collins, M. D., Jones, D. & Kroppenstedt, R. M. ( 1983; ). Reclassification of Brevibacterium imperiale (Steinhaus) and “Corynebacterium laevaniformans” (Dias and Bhat) in a redefined genus Microbacterium (Orla-Jensen), as Microbacterium imperiale comb. nov. and Microbacterium laevaniformans nom. rev.; comb. nov. Syst Appl Microbiol 4, 65–78.[CrossRef]
    [Google Scholar]
  3. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. Cambridge: Cambridge University Press.
  4. Cui, X.-L., Mao, P.-H., Zeng, M., Li, W.-J., Zhang, L.-P., Xu, L.-H. & Jiang, C.-L. ( 2001; ). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363.
    [Google Scholar]
  5. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  6. Deibel, R. H. & Ewans, J. B. ( 1960; ). Modified benzidine test for the detection of cytochrome-containing respiratory systems in microorganisms. J Bacteriol 79, 356–360.
    [Google Scholar]
  7. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  9. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  10. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  11. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  12. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  13. Imai, K., Takeuchi, M. & Banno, I. ( 1984; ). Reclassification of “Flavobacterium arborescens” (Frankland and Frankland) Bergey et al. in the genus Microbacterium (Orla-Jensen) Collins et al., as Microbacterium arborescens comb. nov., nom. rev. Curr Microbiol 11, 281–284.[CrossRef]
    [Google Scholar]
  14. Jahnke, K. D. ( 1992; ). basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef]
    [Google Scholar]
  15. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  16. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  17. Kluge, A. G. & Farris, F. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  18. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  19. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  20. Li, W.-J., Chen, H.-H., Xu, P., Zhang, Y.-Q., Schumann, P., Tang, S.-K., Xu, L.-H. & Jiang, C.-L. ( 2004a; ). Yania halotolerans gen. nov., sp. nov., a novel member of the suborder Micrococcineae from saline soil in China. Int J Syst Evol Microbiol 54, 525–531.[CrossRef]
    [Google Scholar]
  21. Li, W.-J., Chen, H.-H., Zhang, Y.-Q., Schumann, P., Stackebrandt, E., Xu, L.-H. & Jiang, C.-L. ( 2004b; ). Nesterenkonia halotolerans sp. nov. and Nesterenkonia xinjiangensis sp. nov., actinobacteria from saline soils in the west of China. Int J Syst Evol Microbiol 54, 837–841.[CrossRef]
    [Google Scholar]
  22. MacKenzie, S. L. ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160.
    [Google Scholar]
  23. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  24. Marmur, J. & Doty, P. ( 1962; ). Determination of base composition of deoxyribonucleic acid from its denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  25. Minnikin, D. E., Patel, P. V., Alshamaony, L. & Goodfellow, M. ( 1977; ). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27, 104–117.[CrossRef]
    [Google Scholar]
  26. Orla-Jensen, S. ( 1919; ). The Lactic Acid Bacteria. Copenhagen: Høst & Sons.
  27. Rivas, R., Trujillo, M. E., Sánchez, M., Mateos, P. F., Martínez-Molina, E. & Velázquez, E. ( 2004; ). Microbacterium ulmi sp. nov., a xylanolytic, phosphate-solubilizing bacterium isolated from sawdust of Ulmus nigra. Int J Syst Evol Microbiol 54, 513–517.[CrossRef]
    [Google Scholar]
  28. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  29. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  30. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  31. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  32. Takeuchi, M. & Hatano, K. ( 1998; ). Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium. Int J Syst Bacteriol 48, 739–747.[CrossRef]
    [Google Scholar]
  33. Tang, S.-K., Li, W.-J., Dong, W., Zhang, Y.-G., Xu, L.-H. & Jiang, C.-L. ( 2003; ). Studies of the biological characteristics of some halophilic and halotolerant actinomycetes isolated from saline and alkaline soils. Actinomycetologica 17, 6–10.[CrossRef]
    [Google Scholar]
  34. Tarrand, J. J. & Groschel, D. H. M. ( 1982; ). Rapid, modified oxidase test for oxidase-variable bacterial isolates. J Clin Microbiol 16, 772–774.
    [Google Scholar]
  35. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  36. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.2008/001222-0
Loading
/content/journal/ijsem/10.1099/ijs.0.2008/001222-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error