1887

Abstract

Six strains were isolated from root nodules of grown in Nanchang, Yifeng, Taihu, Huaibei, Bengbu and Lujiang, in the middle and lower reaches of the Yangtze River. According to phylogenetic analyses of 16S rRNA gene, and sequences, these strains belong to the genus , with and as the closest related species. CCBAU 33202, a representative of these novel isolates, showed sequence similarity to its closest relatives CFN 42 and USDA 2370 of 99.5 and 99.1 % for the 16S rRNA gene, 91.9 and 91.9 % for and 90.3 and 93.2 % for . The strains from this study could also be differentiated from CFN 42 and USDA 2370 by 16S–IGS RFLP and SDS-PAGE of whole-cell proteins, fatty acid profiles and several phenotypic characteristics. DNA–DNA hybridization yielded relatedness of 19 and 14–43 %, respectively, with CFN 42 and strains representing different biovars of . All data obtained in this study showed that these isolates belong to a novel species, for which the name sp. nov. is proposed. The type strain, CCBAU 33202 (=LMG 23997 =JCM 14381), was isolated from Nanchang.

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2008-12-01
2019-12-05
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vol. , part 12, pp. 2871 - 2875

Neighbour-joining trees based on partial (Fig. S1) and (Fig. S2) gene sequences of strains of sp. nov. and related species.

Dendrograms of 16S–IGS RFLP profiles (Fig. S3) and whole-cell protein SDS-PAGE profiles (Fig. S4) showing the relationship of the novel isolates ( sp. nov.) and related species.

Neighbour-joining tree based on sequences.

DNA–DNA hybridization of sp. nov. CCBAU 33202 with related strains.

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