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Six strains were isolated from root nodules of Vicia faba grown in Nanchang, Yifeng, Taihu, Huaibei, Bengbu and Lujiang, in the middle and lower reaches of the Yangtze River. According to phylogenetic analyses of 16S rRNA gene, atpD and recA sequences, these strains belong to the genus Rhizobium, with Rhizobium etli and Rhizobium leguminosarum as the closest related species. CCBAU 33202T, a representative of these novel isolates, showed sequence similarity to its closest relatives R. etli CFN 42T and R. leguminosarum USDA 2370T of 99.5 and 99.1 % for the 16S rRNA gene, 91.9 and 91.9 % for atpD and 90.3 and 93.2 % for recA. The strains from this study could also be differentiated from R. etli CFN 42T and R. leguminosarum USDA 2370T by 16S–IGS RFLP and SDS-PAGE of whole-cell proteins, fatty acid profiles and several phenotypic characteristics. DNA–DNA hybridization yielded relatedness of 19 and 14–43 %, respectively, with R. etli CFN 42T and strains representing different biovars of R. leguminosarum. All data obtained in this study showed that these V. faba isolates belong to a novel species, for which the name Rhizobium fabae sp. nov. is proposed. The type strain, CCBAU 33202T (=LMG 23997T =JCM 14381T), was isolated from Nanchang.
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 12, pp. 2871 - 2875
Supplementary Figs S1 and S2. Neighbour-joining trees based on partial recA (Fig. S1) and atpD (Fig. S2) gene sequences of strains of Rhizobium fabae sp. nov. and related Rhizobium species.
Supplementary Figs S3 and S4. Dendrograms of 16S–IGS RFLP profiles (Fig. S3) and whole-cell protein SDS-PAGE profiles (Fig. S4) showing the relationship of the novel isolates ( R. fabae sp. nov.) and related Rhizobium species.
Supplementary Fig. S5. Neighbour-joining tree based on nodD sequences.
Supplementary Table S1. DNA–DNA hybridization of R. fabae sp. nov. CCBAU 33202 Twith related strains.
[PDF file of Supplementary Figures and Table](228 KB)