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A Gram-positive, aerobic, neutrophilic and rod-shaped bacterium, strain Shh49T, was isolated from a deep-sea sediment sample collected from the East Pacific polymetallic nodule region. The strain was able to grow within a temperature range of 4–35 °C and tolerated up to 7.5 % (w/v) NaCl. Strain Shh49T was characterized chemotaxonomically by having MK-12 and MK-13 as predominant isoprenoid quinones, anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 as major fatty acids and ornithine as cell-wall diamino acid. The genomic DNA G+C content was 66.8 mol%. On the basis of 16S rRNA gene sequence similarities, the closest phylogenetic neighbours were the type strains of Microbacterium phyllosphaerae (98.3 %) and Microbacterium keratanolyticum (98.0 %), but strain Shh49T could be clearly distinguished from its phylogenetic relatives with reference to a broad range of physiological and biochemical markers. DNA–DNA relatedness of strain Shh49T with M. phyllosphaerae DSM 13468T and M. keratanolyticum DSM 8606T was 56 and 31 %, respectively. On the basis of phenotypic and genotypic data presented in this study, strain Shh49T represents a novel species of the genus Microbacterium, for which the name Microbacterium profundi sp. nov. is proposed. The type strain is Shh49T (=CGMCC 1.6777T =JCM 14840T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 12, pp. 2930 - 2934
Supplementary Fig. S1. Extended neighbour-joining phylogenetic tree based on 16S rRNA gene sequences from all species of the genus Microbacterium .
Supplementary Table S1. Phenotypic characteristics that differentiate strain Shh49 Tfrom its closest phylogenetic neighbours.
Supplementary Table S2. Fatty acid compositions of Microbacterium strains.
[PDF file for Supplementary Figure and Tables](35 KB)