A Gram-positive, aerobic, neutrophilic and rod-shaped bacterium, strain Shh49, was isolated from a deep-sea sediment sample collected from the East Pacific polymetallic nodule region. The strain was able to grow within a temperature range of 4–35 °C and tolerated up to 7.5 % (w/v) NaCl. Strain Shh49 was characterized chemotaxonomically by having MK-12 and MK-13 as predominant isoprenoid quinones, anteiso-C, iso-C, iso-C and anteiso-C as major fatty acids and ornithine as cell-wall diamino acid. The genomic DNA G+C content was 66.8 mol%. On the basis of 16S rRNA gene sequence similarities, the closest phylogenetic neighbours were the type strains of (98.3 %) and (98.0 %), but strain Shh49 could be clearly distinguished from its phylogenetic relatives with reference to a broad range of physiological and biochemical markers. DNA–DNA relatedness of strain Shh49 with DSM 13468 and DSM 8606 was 56 and 31 %, respectively. On the basis of phenotypic and genotypic data presented in this study, strain Shh49 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Shh49 (=CGMCC 1.6777 =JCM 14840).


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vol. , part 12, pp. 2930 - 2934

Extended neighbour-joining phylogenetic tree based on 16S rRNA gene sequences from all species of the genus .

Phenotypic characteristics that differentiate strain Shh49 from its closest phylogenetic neighbours.

Fatty acid compositions of strains.

[PDF file for Supplementary Figure and Tables](35 KB)

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