A coryneform bacterium isolated from the sputum of a patient with pneumonia was characterized by phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and short chain mycolic acids consistent with the genus . Comparative 16S rRNA gene sequencing studies confirmed this assignment, with the organism forming a hitherto unknown subline within the genus associated with a subcluster containing , , , and . Sequence divergence values of >2.7 % from established corynebacterial species suggested that the new isolate represented a novel species. This was also supported by the results of the biochemical tests. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown bacterium be classified as a novel species of the genus , sp. nov. (type strain IMMIB L-999=DSM 45148=CCUG 55795).


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vol. , part 12, pp. 2876 - 2879

Extended neighbour-joining phylogenetic tree showing the position of strain IMMIBL-999 within the radiation of species of the genus . [ PDF] 298 KB

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