1887

International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 12

sp. nov., isolated from the sputum of a patient with pneumonia Free

Abstract

A coryneform bacterium isolated from the sputum of a patient with pneumonia was characterized by phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and short chain mycolic acids consistent with the genus . Comparative 16S rRNA gene sequencing studies confirmed this assignment, with the organism forming a hitherto unknown subline within the genus associated with a subcluster containing , , , and . Sequence divergence values of >2.7 % from established corynebacterial species suggested that the new isolate represented a novel species. This was also supported by the results of the biochemical tests. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown bacterium be classified as a novel species of the genus , sp. nov. (type strain IMMIB L-999=DSM 45148=CCUG 55795).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.2008/000414-0
2008-12-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/12/2876.html?itemId=/content/journal/ijsem/10.1099/ijs.0.2008/000414-0&mimeType=html&fmt=ahah

References

  1. Becker, B., Lechevalier, M. P., Gordon, R. E. & Lechevalier, H. A.(1964). Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole cell hydrolysates. Appl Microbiol 12, 421–423. [Google Scholar]
  2. Collins, M. D., Burton, R. A. & Jones, D.(1988).Corynebacterium amycolatum sp. nov., a new mycolic acid-less Corynebacterium species from human skin. FEMS Microbiol Lett 49, 349–352.[CrossRef] [Google Scholar]
  3. Collins, M. D., Hoyles, L., Foster, G., Sjödén, B. & Falsen, E.(2001).Corynebacterium capitovis sp. nov., from a sheep. Int J Syst Evol Microbiol 51, 857–860.[CrossRef] [Google Scholar]
  4. Collins, M. D., Hoyles, L., Foster, G. & Falsen, E.(2004).Corynebacterium caspium sp. nov., from a Caspian seal (Phoca caspica). Int J Syst Evol Microbiol 54, 925–928.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. Fernández-Garayzábal, J. F., Collins, M. D., Hutson, R. A., González, I., Fernández, E. & Domínguez, L.(1998).Corynebacterium camporealensis sp. nov., associated with subclinical mastitis in sheep. Int J Syst Bacteriol 48, 463–468.[CrossRef] [Google Scholar]
  8. Fernández-Garayzábal, J. F., Vela, A. I., Egido, R., Hutson, R. A., Lanzarot, M. P., Fernández-García, M. & Collins, M. D.(2004).Corynebacterium ciconiae sp. nov., isolated from the trachea of black storks (Ciconia nigra). Int J Syst Evol Microbiol 54, 2191–2195.[CrossRef] [Google Scholar]
  9. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  10. Funke, G., Lawson, P. A. & Collins, M. D.(1997a).Corynebacterium mucifaciens sp. nov., an unusual species from human clinical material. Int J Syst Bacteriol 47, 952–957.[CrossRef] [Google Scholar]
  11. Funke, G., von Graevenitz, A., Clarridge, J. E., III & Bernard, K. A.(1997b). Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 10, 125–159. [Google Scholar]
  12. Funke, G., Osorio, C. R., Frei, R., Riegel, P. & Collins, M. D.(1998).Corynebacterium confusum sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 48, 1291–1296.[CrossRef] [Google Scholar]
  13. Goyache, J., Ballesteros, C., Vela, A. I., Collins, M. D., Briones, V., Hutson, R. A., Potti, J., García-Borboroglu, P., Domínguez, L. & Fernández-Garayzábal, J. F.(2003).Corynebacterium sphenisci sp. nov., isolated from wild penguins. Int J Syst Evol Microbiol 53, 1009–1012.[CrossRef] [Google Scholar]
  14. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  15. Lechevalier, M. P.(1968). Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med 71, 934–944. [Google Scholar]
  16. Lechevalier, M. P. & Lechevalier, H. A.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  17. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  18. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M.(1980). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef] [Google Scholar]
  19. Pascual, C., Lawson, P. A., Farrow, J. A. E., Gimenez, M. N. & Collins, M. D.(1995). Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences. Int J Syst Bacteriol 45, 724–728.[CrossRef] [Google Scholar]
  20. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  21. Renaud, F. N. R., Aubel, D., Riegel, P., Meugnier, H. & Bollet, C.(2001).Corynebacterium freneyi sp. nov., α-glucosidase-positive strains related to Corynebacterium xerosis. Int J Syst Evol Microbiol 51, 1723–1728.[CrossRef] [Google Scholar]
  22. Renaud, F. N. R., Le Coustumier, A., Wilhelm, N., Aubel, D., Riegel, P., Bollet, C. & Freney, J.(2007).Corynebacterium hansenii sp. nov., an α-glucosidase-negative bacterium related to Corynebacterium xerosis. Int J Syst Evol Microbiol 57, 1113–1116.[CrossRef] [Google Scholar]
  23. Riegel, P., de Briel, D., Pre'vost, G., Jehl, F. & Monteil, H.(1994). Genomic diversity among Corynebacterium jeikeium strains and comparison with biochemical characteristics. J Clin Microbiol 32, 1860–1865. [Google Scholar]
  24. Ruimy, R., Riegel, P., Boiron, P., Monteil, H. & Christen, R.(1995). Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences. Int J Syst Bacteriol 45, 740–746.[CrossRef] [Google Scholar]
  25. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  26. Stackebrandt, E. & Ebers, J.(2006). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155. [Google Scholar]
  27. Yassin, A. F., Steiner, U. & Ludwig, W.(2002a).Corynebacterium aurimucosum sp. nov. and emended description of Corynebacterium minutissimum Collins and Jones (1983). Int J Syst Evol Microbiol 52, 1001–1005.[CrossRef] [Google Scholar]
  28. Yassin, A. F., Steiner, U. & Ludwig, W.(2002b).Corynebacterium appendicis sp. nov. Int J Syst Evol Microbiol 52, 1165–1169.[CrossRef] [Google Scholar]
  29. Yassin, A. F., Chen, W. M., Hupfer, H., Siering, C., Kroppenstedt, R. M., Arun, A. B., Lai, W. A., Shen, F. T., Rekha, P. D. & Young, C. C.(2007).Lysobacter defluvii sp. nov., isolated from municipal solid waste. Int J Syst Evol Microbiol 57, 1131–1136.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.2008/000414-0
Loading
Corynebacterium sputi sp. nov., isolated from the sputum of a patient with pneumonia
Int J Syst Evol Microbiol 58, 2876 (2008); https://doi.org/10.1099/ijs.0.2008/000414-0
/content/journal/ijsem/10.1099/ijs.0.2008/000414-0
/content/journal/ijsem/10.1099/ijs.0.2008/000414-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 2876 - 2879

Extended neighbour-joining phylogenetic tree showing the position of strain IMMIBL-999 within the radiation of species of the genus . [ PDF] 298 KB

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error