A Gram-positive bacterium, designated strain CW 7, was isolated from forest soil in Anhui Province, south-east China. Cells were strictly aerobic, motile with peritrichous flagella and rod-shaped. The strain grew optimally at 30–37 °C and pH 7.0–8.0. The major fatty acids of strain CW 7 were anteiso-C, iso-C and anteiso-C. The predominant menaquinone was MK-7. The cell-wall peptidoglycan contained -diaminopimelic acid. The G+C content of the genomic DNA was 42.3 mol%. Phylogenetic analysis indicated that strain CW 7 belonged to a monophyletic cluster within the genus and showed 16S rRNA gene sequence similarities of less than 96.5 % to recognized species of the genus . The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analyses, showed that strain CW 7 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CW 7 (=KCTC 13200=CCTCC AB 207188=LMG 24451).


Article metrics loading...

Loading full text...

Full text loading...



  1. Ahmed, I., Yokota, A. & Fujiwara, T.(2007). A novel highly boron tolerant bacterium, Bacillus boroniphilus sp. nov., isolated from soil, that requires boron for its growth. Extremophiles 11, 217–224.[CrossRef] [Google Scholar]
  2. Albert, R. A., Archambault, J., Ramón, R.-M., Tindall, B. J. & Matheny, M.(2005).Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55, 2125–2130.[CrossRef] [Google Scholar]
  3. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  4. Heyrman, J., Logan, N. A., Marina, R.-D., Scheldeman, P., Lebbe, L., Swings, J., Heyndrickx, M. & De Vos, P. D.(2005). Study of mural painting isolates, leading to the transfer of ‘Bacillus maroccanus’ and ‘Bacillus carotarum’ to Bacillus simplex, emended description of Bacillus simplex, re-examination of the strains previously attributed to ‘Bacillus macroides’ and description of Bacillus muralis sp. nov. Int J Syst Evol Microbiol 55, 119–131.[CrossRef] [Google Scholar]
  5. Hu, H.-Y., Lim, B.-R., Naohiro, G. & Koich, F.-J.(2001). Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. J Microbiol Methods 47, 17–24.[CrossRef] [Google Scholar]
  6. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  7. Kimura, M.(1983).The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University.
  8. Ko, K. S., Oh, W. S., Lee, M. Y., Lee, J. H., Lee, H., Peck, K. R., Lee, N. Y. & Song, J.-H.(2006).Bacillus infantis sp. nov. and Bacillus idriensis sp. nov., isolated from a patient with neonatal sepsis. Int J Syst Evol Microbiol 56, 2541–2544.[CrossRef] [Google Scholar]
  9. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  11. Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L.(2007).Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef] [Google Scholar]
  12. Lim, J.-M., Jeon, C. O., Lee, J.-C., Ju, Y. J., Park, D.-J. & Kim, C.-J.(2006).Bacillus koreensis sp. nov., a spore-forming bacterium, isolated from the rhizosphere of willow roots in Korea. Int J Syst Evol Microbiol 56, 59–63.[CrossRef] [Google Scholar]
  13. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  15. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  16. Nakamura, L. K., Roberts, M. S. & Cohan, F. M.(1999). Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov. Int J Syst Bacteriol 49, 1211–1215.[CrossRef] [Google Scholar]
  17. Pettersson, B., Lembke, F., Hammer, P., Stackebrandt, E. & Priest, F. G.(1996).Bacillus sporothermodurans, a new species producing highly heat-resistant endospores. Int J Syst Bacteriol 46, 759–764.[CrossRef] [Google Scholar]
  18. Pettersson, B., Silva, S. K., Uhlén, M. & Priest, F. G.(2000).Bacillus siralis sp. nov., a novel species from silage with a higher order structural attribute in the 16S rRNA genes. Int J Syst Evol Microbiol 50, 2181–2187.[CrossRef] [Google Scholar]
  19. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  20. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  21. Suresh, K., Prabagaran, S. R., Sengupta, S. & Shivaji, S.(2004).Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 54, 1369–1375.[CrossRef] [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  23. Venkateswaran, K., Kempf, M., Chen, F., Satomi, M., Nicholson, W. & Kern, R.(2003).Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are γ-radiation resistant. Int J Syst Evol Microbiol 53, 165–172.[CrossRef] [Google Scholar]
  24. Yoon, J. H., Kim, I. G., Kang, K. H., Oh, T. K. & Park, Y. H.(2003).Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 53, 1297–1303.[CrossRef] [Google Scholar]
  25. Zhou, Y., Dong, J., Wang, X., Huang, X., Zhang, K. Y., Zhang, Y.-Q., Guo, Y.-F., Lai, R. & Li, W.-J.(2007).Chryseobacterium flavum sp. nov., isolated from a polluted soil. Int J Syst Evol Microbiol 57, 1765–1769.[CrossRef] [Google Scholar]

Data & Media loading...


Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences, showing the position of strain CW 7 ( sp. nov.). [PDF](18 KB)


Two-dimensional TLC of a polar lipid extract from strain CW 7 ( sp. nov.). [PDF](30 KB)


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error