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Abstract

A novel psychrophilic bacterium, designated strain DVS 3Y, was isolated from a moraine sample from the McMurdo Dry Valleys, Antarctica. Phenotypic and chemotaxonomic characteristics and data from a phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DVS 3Y was related to the genus . Strain DVS 3Y exhibited >97 % gene sequence similarity with respect to DSM 20416 (97.4 %), DSM 17272 (97.4 %), IAM 15368 (97.6 %), DSM 14481 (98.2 %), DSM 17290 (98.6 %) and DSM 14480 (99.6 %). However, the levels of DNA–DNA relatedness between strain DVS 3Y and DSM 20416, JCM 12280, IAM 15368, DSM 14481, DSM 17290 and DSM 14480 were less than 70 % (52, 25, 16, 33, 16 and 38 %, respectively). Strain DVS 3Y also differed from these six closely related species in a number of phenotypic traits. Phenotypic, chemotaxonomic and phylogenetic data suggest that strain DVS 3Y represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DVS 3Y (=MTCC 4816=JCM 14376).

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2008-10-01
2020-01-25
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References

  1. Chaturvedi, P. & Shivaji, S. ( 2006; ). Exiguobacterium indicum sp. nov., a psychrophilic bacterium from the Hamta glacier of the Himalayan mountain ranges of India. Int J Syst Evol Microbiol 56, 2765–2770.[CrossRef]
    [Google Scholar]
  2. Chaturvedi, P., Reddy, G. S. N. & Shivaji, S. ( 2005; ). Dyadobacter hamtensis sp. nov., from Hamta glacier, located in the Himalayas, India. Int J Syst Evol Microbiol 55, 2113–2117.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  4. Collins, M. D., Lund, B. M., Farrow, J. A. E. & Schleifer, K. ( 1983; ). Chemotaxonomic study of an alkalophilic bacterium, Exiguobacterium aurantiacum gen. nov., sp. nov. J Gen Microbiol 129, 2037–2042.
    [Google Scholar]
  5. Farrow, J. A. E., Wallbanks, S. & Collins, M. D. ( 1994; ). Phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera Caryophanon, Exiguobacterium, Kurthia and Planococcus. Int J Syst Bacteriol 44, 74–82.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package) version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. Fruhling, A., Schumann, P., Hippe, H., Straubler, B. & Stackebrandt, E. ( 2002; ). Exiguobacterium undae sp. nov. and Exiguobacterium antarcticum sp. nov. Int J Syst Evol Microbiol 52, 1171–1176.[CrossRef]
    [Google Scholar]
  8. Gee, J. M., Lund, B. M., Metcalf, G. & Peel, J. L. ( 1980; ). Properties of a new group of alkalophilic bacteria. J Gen Microbiol 117, 9–17.
    [Google Scholar]
  9. Hugh, R. & Leifson, E. ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66, 24–26.
    [Google Scholar]
  10. Kim, I.-G., Lee, M.-H., Jung, S.-Y., Song, J. J., Oh, T.-K. & Yoon, J.-H. ( 2005; ). Exiguobacterium aestuarii sp. nov. and Exiguobacterium marinum sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 55, 885–889.[CrossRef]
    [Google Scholar]
  11. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  12. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  13. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  14. Lopez-Cortes, A., Schumann, P., Pukall, R. & Stackebrandt, E. ( 2006; ). Exiguobacterium mexicanum sp. nov. and Exiguobacterium artemiae sp. nov. isolated from the brine shrimp Artemia franciscana. Syst Appl Microbiol 29, 183–190.[CrossRef]
    [Google Scholar]
  15. Reddy, G. S. N., Matsumoto, G. I. & Shivaji, S. ( 2003; ). Sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the McMurdo Dry Valleys, Antarctica. Int J Syst Evol Microbiol 53, 1363–1367.[CrossRef]
    [Google Scholar]
  16. Rodrigues, D. F., Goris, J., Vishnivetskaya, T., Gilichinsky, D., Thomashow, M. F. & Tiedje, J. M. ( 2006; ). Characterization of Exiguobacterium isolates from the Siberian permafrost. Description of Exiguobacterium sibiricum sp. nov. Extremophiles 10, 285–294.[CrossRef]
    [Google Scholar]
  17. Sato, N. S. & Murata, N. ( 1988; ). Membrane lipids. In Methods in Enzymology, vol. 167, pp. 251–259, Edited by L. Packer & A. N. Glazer. New York: Academic Press.
  18. Shivaji, S., Rao, N. S., Saisree, L., Reddy, G. S. N., Seshu Kumar, G. & Bhargava, P. M. ( 1989; ). Isolates of Arthrobacter from the soils of Schirmacher Oasis, Antarctica. Polar Biol 10, 225–229.
    [Google Scholar]
  19. Shivaji, S., Ray, M. K., Rao, N. S., Saisree, L., Jagannadham, M. V., Kumar, G. S., Reddy, G. S. N. & Bhargava, P. M. ( 1992; ). Sphingobacterium antarcticus sp. nov. a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42, 102–106.[CrossRef]
    [Google Scholar]
  20. Shivaji, S., Vijaya Bhanu, N. & Aggarwal, R. K. ( 2000; ). Identification of Yersinia pestis as the causative organism of plague in India as determined by 16S rDNA sequencing and RAPD-based genomic fingerprinting. FEMS Microbiol Lett 189, 247–252.[CrossRef]
    [Google Scholar]
  21. Shivaji, S., Reddy, G. S. N., Raghavan, P. U. M., Sarita, N. B. & Delille, D. ( 2004; ). Psychrobacter salsus sp. nov. and Psychrobacter adeliensis sp. nov. isolated from fast ice from Adelie Land, Antarctica. Syst Appl Microbiol 27, 628–635.[CrossRef]
    [Google Scholar]
  22. Shivaji, S., Chaturvedi, P., Reddy, G. S. N. & Suresh, K. ( 2005; ). Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India. Int J Syst Evol Microbiol 55, 1083–1088.[CrossRef]
    [Google Scholar]
  23. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt. Washington, DC: American Society for Microbiology.
  24. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  25. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  26. Tourova, T. P. & Antonov, A. S. ( 1987; ). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19, 333–355.
    [Google Scholar]
  27. Yumoto, I., Narisawa, M. H., Hirota, K., Shingyo, T., Takebe, F., Nodasaka, Y., Matsuyama, H. & Hara, I. ( 2004; ). Exiguobacterium oxidotolerans sp. nov., a novel alkaliphile exhibiting high catalase activity. Int J Syst Evol Microbiol 54, 2013–2017.[CrossRef]
    [Google Scholar]
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vol. , part 10, pp. 2447 - 2453

Comparison of fatty acid compositions (%) of strain DVS 3Y and related species of the genus . [ PDF] 99 KB



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