1887

Abstract

A Gram-stain-negative, flagellated, aerobic and rod-shaped or ovoid bacterial strain, designated HJTF-7, was isolated from a tidal flat on the South Sea of South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain HJTF-7 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees, based on 16S rRNA gene sequences, showed that strain HJTF-7 joined the cluster comprising the type strains of species of the genera and . Strain HJTF-7 exhibited 16S rRNA gene sequence similarities of 90.4–92.5 % to the type strains of species of the genera and and of less than 91.5 % to the type strains of other recognized species. Strain HJTF-7 contained Q-8 as the predominant ubiquinone. The major fatty acids were iso-Cω9, iso-C, iso-C, iso-C 3-OH and Cω8 and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The fatty acid and polar lipid profiles of strain HJTF-7 were distinct from those of members of the genera and . The DNA G+C content of strain HJTF-7 was 55.9 mol%. The phylogenetic data and differential chemotaxonomic and other phenotypic properties revealed that strain HJTF-7 represents a novel genus and species within the class , for which the name gen. nov., sp. nov. is proposed. The type strain of is HJTF-7 ( = KCTC 42157 = CECT 8654).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.069500-0
2015-01-01
2019-11-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/1/141.html?itemId=/content/journal/ijsem/10.1099/ijs.0.069500-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A.. (editors) ( 1993;). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  2. Baumann P., Baumann L.. ( 1981;). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G... Berlin:: Springer;.
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L.. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef][PubMed]
    [Google Scholar]
  4. Buchan A., González J. M., Moran M. A.. ( 2005;). Overview of the marine roseobacter lineage. . Appl Environ Microbiol 71:, 5665–5677. [CrossRef][PubMed]
    [Google Scholar]
  5. Cohen-Bazire G., Sistrom W. R., Stanier R. Y.. ( 1957;). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49:, 25–68. [CrossRef]
    [Google Scholar]
  6. Embley T. M., Wait R.. ( 1994;). Structural lipids of eubacteria. . In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M., O’Donnell A. G... Chichester:: Wiley;.
    [Google Scholar]
  7. Garrity G. M., Bell J. A., Lilburn T.. ( 2005;). Family I. Rhodobacteraceae fam. nov. In Bergey’s Manual of Systematic Bacteriology, 2nd edn, vol. 2 (The Proteobacteria part C, The Alpha-, Beta-, Delta, and Epsilonproteobacteria), pp. 161. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer..
  8. Garrity G. M., Bell J. A., Lilburn T.. ( 2006;). Family I. Rhodobacteraceae fam. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 107. . Int J Syst Evol Microbiol 56:, 1–6. [CrossRef][PubMed]
    [Google Scholar]
  9. Graeber I., Kaesler I., Borchert M. S., Dieckmann R., Pape T., Lurz R., Nielsen P., von Döhren H., Michaelis W., Szewzyk U.. ( 2008;). Spongiibacter marinus gen. nov., sp. nov., a halophilic marine bacterium isolated from the boreal sponge Haliclona sp. 1. . Int J Syst Evol Microbiol 58:, 585–590. [CrossRef][PubMed]
    [Google Scholar]
  10. Hwang C. Y., Cho B. C.. ( 2009;). Spongiibacter tropicus sp. nov., isolated from a Synechococcus culture. . Int J Syst Evol Microbiol 59:, 2176–2179. [CrossRef][PubMed]
    [Google Scholar]
  11. Jang G. I., Hwang C. Y., Choi H.-G., Kang S.-H., Cho B. C.. ( 2011;). Description of Spongiibacter borealis sp. nov., isolated from Arctic seawater, and reclassification of Melitea salexigens Urios et al. 2008 as a later heterotypic synonym of Spongiibacter marinus Graeber et al. 2008 with emended descriptions of the genus Spongiibacter and Spongiibacter marinus. . Int J Syst Evol Microbiol 61:, 2895–2900. [CrossRef][PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  13. Lányí B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  14. Lee S.-Y., Park S., Oh T.-K., Yoon J.-H.. ( 2013;). Kangiella sediminilitoris sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 63:, 1001–1006. [CrossRef][PubMed]
    [Google Scholar]
  15. Li H.-J., Zhang X.-Y., Chen C.-X., Zhang Y.-J., Gao Z.-M., Yu Y., Chen X.-L., Chen B., Zhang Y.-Z.. ( 2011;). Zhongshania antarctica gen. nov., sp. nov. and Zhongshania guokunii sp. nov., gammaproteobacteria respectively isolated from coastal attached (fast) ice and surface seawater of the Antarctic. . Int J Syst Evol Microbiol 61:, 2052–2057. [CrossRef][PubMed]
    [Google Scholar]
  16. Lo N., Kang H. J., Jeon C. O.. ( 2014;). Zhongshania aliphaticivorans sp. nov., an aliphatic hydrocarbon degrading bacterium isolated from marine sediment, and transfer of Spongiibacter borealis Jang et al. 2011 to the genus Zhongshania as Zhongshania borealis comb. nov. . Int J Syst Evol Microbiol . [CrossRef] [Epub ahead of print][PubMed]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  18. Park S., Yoon J.-H.. ( 2013;). Roseovarius sediminilitoris sp. nov., isolated from seashore sediment. . Int J Syst Evol Microbiol 63:, 1741–1745. [CrossRef][PubMed]
    [Google Scholar]
  19. Park S., Jung Y.-T., Yoon J.-H.. ( 2013;). Loktanella sediminilitoris sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 63:, 4118–4123. [CrossRef][PubMed]
    [Google Scholar]
  20. Park S., Park D.-S., Bae K. S., Yoon J.-H.. ( 2014;). Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. . Int J Syst Evol Microbiol 64:, 1378–1383. [CrossRef][PubMed]
    [Google Scholar]
  21. Rappé M. S., Vergin K., Giovannoni S. J.. ( 2000;). Phylogenetic comparisons of a coastal bacterioplankton community with its counterparts in open ocean and freshwater systems. . FEMS Microbiol Ecol 33:, 219–232. [CrossRef][PubMed]
    [Google Scholar]
  22. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc..
  23. Staley J. T.. ( 1968;). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95:, 1921–1942.[PubMed]
    [Google Scholar]
  24. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H.. ( 1996;). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  26. Yoon J.-H., Lee S. T., Park Y.-H.. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef][PubMed]
    [Google Scholar]
  27. Yoon J.-H., Kang K. H., Park Y.-H.. ( 2003;). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53:, 449–454. [CrossRef][PubMed]
    [Google Scholar]
  28. Yoon J.-H., Lee S.-Y., Jung Y.-T., Lee J.-S., Lee K.-C.. ( 2013a;). Litorisediminicola beolgyonensis gen. nov., sp. nov., isolated from a coastal sediment. . Int J Syst Evol Microbiol 63:, 2025–2031. [CrossRef][PubMed]
    [Google Scholar]
  29. Yoon J.-H., Park S., Jung Y.-T.. ( 2013b;). Aestuariihabitans beolgyonensis gen. nov., sp. nov., a novel alphaproteobacterium isolated from tidal flat sediment. . Antonie van Leeuwenhoek 104:, 217–224. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.069500-0
Loading
/content/journal/ijsem/10.1099/ijs.0.069500-0
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error