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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 65, Issue Pt_1

gen. nov., sp. nov., a lipolytic bacterium isolated from a tidal flat Free

Abstract

A Gram-stain-negative, flagellated, aerobic and rod-shaped or ovoid bacterial strain, designated HJTF-7, was isolated from a tidal flat on the South Sea of South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain HJTF-7 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees, based on 16S rRNA gene sequences, showed that strain HJTF-7 joined the cluster comprising the type strains of species of the genera and . Strain HJTF-7 exhibited 16S rRNA gene sequence similarities of 90.4–92.5 % to the type strains of species of the genera and and of less than 91.5 % to the type strains of other recognized species. Strain HJTF-7 contained Q-8 as the predominant ubiquinone. The major fatty acids were iso-Cω9, iso-C, iso-C, iso-C 3-OH and Cω8 and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The fatty acid and polar lipid profiles of strain HJTF-7 were distinct from those of members of the genera and . The DNA G+C content of strain HJTF-7 was 55.9 mol%. The phylogenetic data and differential chemotaxonomic and other phenotypic properties revealed that strain HJTF-7 represents a novel genus and species within the class , for which the name gen. nov., sp. nov. is proposed. The type strain of is HJTF-7 ( = KCTC 42157 = CECT 8654).

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Funding
This study was supported by the:
  • Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea (Award 2012R1A2A2A01047369)
© 2015 IUMS
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2015-01-01
2025-01-26
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Litorivivens lipolytica gen. nov., sp. nov., a lipolytic bacterium isolated from a tidal flat
Int J Syst Evol Microbiol 65, 141 (2015); https://doi.org/10.1099/ijs.0.069500-0
/content/journal/ijsem/10.1099/ijs.0.069500-0
/content/journal/ijsem/10.1099/ijs.0.069500-0
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