1887

Abstract

During the course of screening bacterial isolates as sources of as-yet unknown bioactive compounds with pharmaceutical applications, a chemo-organotrophic, Gram-negative bacterium was isolated from a soil sample taken from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain. Strain F-278,770 was oxidase- and catalase-positive, aerobic, with a respiratory type of metabolism with oxygen as the terminal electron acceptor, non-spore-forming and motile by one polar flagellum, although some cells had two polar flagella. Phylogenetic analysis of the 16S rRNA, , and genes revealed that strain F-278,770 belongs to the subgroup ( lineage), with , , and as its closest relatives. Chemotaxonomic traits such as polar lipid and fatty acid compositions and G+C content of genomic DNA corroborated the placement of strain F-278,770 in the genus . DNA–DNA hybridization assays and phenotypic traits confirmed that this strain represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is F-278,770 ( = DSM 28040 = LMG 27940).

Funding
This study was supported by the:
  • Non-Oriented Basic Research Program of the Spanish Ministry of Science (Award project SAF2010-15010)
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2015-02-01
2024-12-04
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References

  1. Ait Tayeb L. A., Ageron E., Grimont F., Grimont P. A. ( 2005 ). Molecular phylogeny of the genus Pseudomonas based on rpoB sequences and application for the identification of isolates. . Res Microbiol 156, 763773. [View Article] [PubMed]
    [Google Scholar]
  2. Cámara B., Strömpl C., Verbarg S., Spröer C., Pieper D. H., Tindall B. J. ( 2007 ). Pseudomonas reinekei sp. nov., Pseudomonas moorei sp. nov. and Pseudomonas mohnii sp. nov., novel species capable of degrading chlorosalicylates or isopimaric acid. . Int J Syst Evol Microbiol 57, 923931. [View Article] [PubMed]
    [Google Scholar]
  3. Davis K. E. R., Joseph S. J., Janssen P. H. ( 2005 ). Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria. . Appl Environ Microbiol 71, 826834. [View Article] [PubMed]
    [Google Scholar]
  4. Heimbrook M. E., Wang W. L., Campbell G. ( 1989 ). Staining bacterial flagella easily. . J Clin Microbiol 27, 26122615.[PubMed]
    [Google Scholar]
  5. Joseph S. J., Hugenholtz P., Sangwan P., Osborne C. A., Janssen P. H. ( 2003 ). Laboratory cultivation of widespread and previously uncultured soil bacteria. . Appl Environ Microbiol 69, 72107215. [View Article] [PubMed]
    [Google Scholar]
  6. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  7. Kim M., Oh H. S., Park S. C., Chun J. ( 2014 ). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. . Int J Syst Evol Microbiol 64, 346351. [View Article] [PubMed]
    [Google Scholar]
  8. King E. O., Ward M. K., Raney D. E. ( 1954 ). Two simple media for the demonstration of pyocyanin and fluorescin. . J Lab Clin Med 44, 301307.[PubMed]
    [Google Scholar]
  9. Kwon S. W., Kim J. S., Park I. C., Yoon S. H., Park D. H., Lim C. K., Go S. J. ( 2003 ). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. . Int J Syst Evol Microbiol 53, 2127. [CrossRef]
    [Google Scholar]
  10. Lin S. Y., Hameed A., Liu Y. C., Hsu Y. H., Lai W. A., Chen W. M., Shen F. T., Young C. C. ( 2013a ). Pseudomonas sagittaria sp. nov., a siderophore-producing bacterium isolated from oil-contaminated soil. . Int J Syst Evol Microbiol 63, 24102417. [View Article] [PubMed]
    [Google Scholar]
  11. Lin S. Y., Hameed A., Liu Y. C., Hsu Y. H., Lai W. A., Young C. C. ( 2013b ). Pseudomonas formosensis sp. nov., a gamma-proteobacteria isolated from food-waste compost in Taiwan. . Int J Syst Evol Microbiol 63, 31683174. [View Article] [PubMed]
    [Google Scholar]
  12. Liu Y. C., Young L. S., Lin S. Y., Hameed A., Hsu Y. H., Lai W. A., Shen F. T., Young C. C. ( 2013 ). Pseudomonas guguanensis sp. nov., a gammaproteobacterium isolated from a hot spring. . Int J Syst Evol Microbiol 63, 45914598. [View Article] [PubMed]
    [Google Scholar]
  13. López J. R., Diéguez A. L., Doce A., De la Roca E., De la Herran R., Navas J. I., Toranzo A. E., Romalde J. L. ( 2012 ). Pseudomonas baetica sp. nov., a fish pathogen isolated from wedge sole, Dicologlossa cuneata (Moreau). . Int J Syst Evol Microbiol 62, 874882. [View Article] [PubMed]
    [Google Scholar]
  14. Martin D. P., Lemey P., Lott M., Moulton V., Posada D., Lefeuvre P. ( 2010 ). RDP3: a flexible and fast computer program for analyzing recombination. . Bioinformatics 26, 24622463. [View Article] [PubMed]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  16. Moore E. R. B., Tindall B. J., Martins Dos Santos V. A. P., Pieper D. H., Ramos J.-L., Palleroni N. J. ( 2006 ). Nonmedical: Pseudomonas . . In The Prokaryotes, , 3rd edn., vol. 6, pp. 646703. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;. [View Article]
    [Google Scholar]
  17. Mulet M., Lalucat J., García-Valdés E. ( 2010 ). DNA sequence-based analysis of the Pseudomonas species. . Environ Microbiol 12, 15131530.[PubMed]
    [Google Scholar]
  18. Mulet M., Gomila M., Scotta C., Sánchez D., Lalucat J., García-Valdés E. ( 2012 ). Concordance between whole-cell matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and multilocus sequence analysis approaches in species discrimination within the genus Pseudomonas . . Syst Appl Microbiol 35, 455464. [View Article] [PubMed]
    [Google Scholar]
  19. Oyaizu H., Komagata K. ( 1983 ). Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty acids. . J Gen Appl Microbiol 29, 1740. [View Article]
    [Google Scholar]
  20. Pascual J., Macián M. C., Arahal D. R., Garay E., Pujalte M. J. ( 2010 ). Multilocus sequence analysis of the central clade of the genus Vibrio by using the 16S rRNA, recA, pyrH, rpoD, gyrB, rctB and toxR genes. . Int J Syst Evol Microbiol 60, 154165. [View Article] [PubMed]
    [Google Scholar]
  21. Pascual J., Lucena T., Ruvira M. A., Giordano A., Gambacorta A., Garay E., Arahal D. R., Pujalte M. J., Macián M. C. ( 2012 ). Pseudomonas litoralis sp. nov., isolated from Mediterranean seawater. . Int J Syst Evol Microbiol 62, 438444. [View Article] [PubMed]
    [Google Scholar]
  22. Powers E. M. ( 1995 ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. . Appl Environ Microbiol 61, 37563758.[PubMed]
    [Google Scholar]
  23. Ramírez-Bahena M. H., Cuesta M. J., Flores-Félix J. D., Mulas R., Rivas R., Castro-Pinto J., Brañas J., Mulas D., González-Andrés F. & other authors ( 2014 ). Pseudomonas helmanticensis sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 64, 23382345. [View Article] [PubMed]
    [Google Scholar]
  24. Ramos E., Ramírez-Bahena M. H., Valverde A., Velázquez E., Zúñiga D., Velezmoro C., Peix A. ( 2013 ). Pseudomonas punonensis sp. nov., isolated from straw. . Int J Syst Evol Microbiol 63, 18341839. [View Article] [PubMed]
    [Google Scholar]
  25. Sait M., Hugenholtz P., Janssen P. H. ( 2002 ). Cultivation of globally distributed soil bacteria from phylogenetic lineages previously only detected in cultivation-independent surveys. . Environ Microbiol 4, 654666. [View Article] [PubMed]
    [Google Scholar]
  26. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  27. Schaeffer A. B., Fulton M. D. ( 1933 ). A simplified method of staining endospores. . Science 77, 194. [View Article] [PubMed]
    [Google Scholar]
  28. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  29. Tao Y., Zhou Y., He X., Hu X., Li D. ( 2014 ). Pseudomonas chengduensis sp. nov., isolated from landfill leachate. . Int J Syst Evol Microbiol 64, 95100.[PubMed] [CrossRef]
    [Google Scholar]
  30. Toro M., Ramírez-Bahena M. H., Cuesta M. J., Velázquez E., Peix A. ( 2013 ). Pseudomonas guariconensis sp. nov., isolated from rhizospheric soil. . Int J Syst Evol Microbiol 63, 44134420. [View Article] [PubMed]
    [Google Scholar]
  31. Tvrzová L., Schumann P., Spröer C., Sedlácek I., Pácová Z., Šedo O., Zdráhal Z., Steffen M., Lang E. ( 2006 ). Pseudomonas moraviensis sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds, and emended description of Pseudomonas asplenii . . Int J Syst Evol Microbiol 56, 26572663. [View Article] [PubMed]
    [Google Scholar]
  32. Urdiain M., López-López A., Gonzalo C., Busse H.-J., Langer S., Kämpfer P., Rosselló-Móra R. ( 2008 ). Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov., a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium, Rhodobium orientis and Rhodobium gokarnense . . Syst Appl Microbiol 31, 339351. [View Article] [PubMed]
    [Google Scholar]
  33. Vancanneyt M., Witt S., Abraham W.-R., Kersters K., Fredrickson H. L. ( 1996 ). Fatty acid content in whole-cell hydrolysates and phospholipid fractions of pseudomonads: a taxonomic evaluation. . Syst Appl Microbiol 19, 528540. [View Article]
    [Google Scholar]
  34. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. C., Murray R. G. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  35. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
  36. Xie F., Ma H., Quan S., Liu D., Chen G., Chao Y., Qian S. ( 2014 ). Pseudomonas kunmingensis sp. nov., an exopolysaccharide-producing bacterium isolated from a phosphate mine. . Int J Syst Evol Microbiol 64, 559564. [View Article] [PubMed]
    [Google Scholar]
  37. Yang G., Han L., Wen J., Zhou S. ( 2013 ). Pseudomonas guangdongensis sp. nov., isolated from an electroactive biofilm, and emended description of the genus Pseudomonas Migula 1894. . Int J Syst Evol Microbiol 63, 45994605. [View Article] [PubMed]
    [Google Scholar]
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