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Abstract

A floc-forming, Gram-stain-negative, petroleum hydrocarbon-degrading bacterial strain, designated Buc, was isolated from a petroleum hydrocarbon-contaminated site in Hungary. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Buc formed a distinct phyletic lineage within the genus . Its closest relative was found to be EMB43 (97.2 % 16S rRNA gene sequence similarity) followed by A-7 (95.9 %), ATCC 19544 (95.5 %) and DhA-35 (95.4 %). The level of DNA–DNA relatedness between strain Buc and EMB43 was 31.6 %. Cells of strain Buc are facultatively aerobic, rod-shaped, and motile by means of a polar flagellum. The strain grew at temperatures of 5–35 °C (optimum 25–28 °C), and at pH 6.0–9.0 (optimum 6.5–7.5). The predominant fatty acids were C, C 3-OH, C and summed feature 3 (Cω7 and/or iso-C 2-OH). The major respiratory quinone was ubiquinone-8 (Q-8) and the predominant polar lipid was phosphatidylethanolamine. The genomic DNA G+C content was 63.2 mol%. On the basis of the chemotaxonomic, molecular and phenotypic data, isolate Buc is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Buc ( = DSM 28387 = NCAIM B 02570).

Funding
This study was supported by the:
  • , Hungarian Academy of Sciences
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2015-01-01
2020-09-26
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