1887

Abstract

In a taxonomic study of yeasts recovered from nectar of flowers and associated insects in South Africa, 11 strains were found to represent two novel species. Morphological and physiological characteristics and sequence analyses of the large-subunit rRNA gene D1/D2 region, as well as the actin, RNA polymerase II and elongation factor 2 genes, showed that the two novel species belonged to the genus . sp. nov. (type strain EBD-CdVSA09-2 = CBS 13649 = NRRL Y-63721; MycoBank no. MB809688; allotype EBD-CdVSA10-2 = CBS13650 = NRRL Y-63720) was recovered from nectar of and the beetle sp. This species belongs to the large-spored clade and is closely related to , with which mating reactions and single-spored asci were observed. sp. nov. (type strain EBD-CdVSA08-1 = CBS 13651 = NRRL Y-63722; MycoBank no. MB809689; allotype EBD-CdVSA57-2 = CBS 13729 = NRRL Y-63723) was isolated from nectar of , and and a honeybee, and is a sister species to and . Analyses of the four sequences demonstrated the existence of three separate phylotypes. Intraspecies matings led to the production of mature asci of unprecedented morphology, with a long, flexuous tail. A single ascospore was produced in all compatible crosses, regardless of sequence phylotype. The two species appear to be endemic to South Africa. The ecology and habitat specificity of these novel species are discussed in terms of host plant and insect host species.

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2014-11-01
2019-11-17
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References

  1. Belisle M., Peay K. G., Fukami T.. ( 2012;). Flowers as islands: spatial distribution of nectar-inhabiting microfungi among plants of Mimulus aurantiacus, a hummingbird-pollinated shrub. . Microb Ecol 63:, 711–718. [CrossRef][PubMed]
    [Google Scholar]
  2. Brysch-Herzberg M.. ( 2004;). Ecology of yeasts in plant-bumblebee mutualism in Central Europe. . FEMS Microbiol Ecol 50:, 87–100. [CrossRef][PubMed]
    [Google Scholar]
  3. de Vega C., Herrera C. M.. ( 2012;). Relationships among nectar-dwelling yeasts, flowers and ants: patterns and incidence on nectar traits. . Oikos 121:, 1878–1888. [CrossRef]
    [Google Scholar]
  4. de Vega C., Herrera C. M.. ( 2013;). Microorganisms transported by ants induce changes in floral nectar composition of an ant-pollinated plant. . Am J Bot 100:, 792–800. [CrossRef][PubMed]
    [Google Scholar]
  5. de Vega C., Guzmán B., Lachance M.-A., Steenhuisen S.-L., Johnson S. D., Herrera C. M.. ( 2012;). Metschnikowia proteae sp. nov., a nectarivorous insect-associated yeast species from Africa. . Int J Syst Evol Microbiol 62:, 2538–2545. [CrossRef][PubMed]
    [Google Scholar]
  6. Dupont Y. L., Hansen D. M., Rasmussen J. T., Olesen J. M.. ( 2004;). Evolutionary changes in nectar sugar composition associated with switches between bird and insect pollination: the Canarian bird-flower element revisited. . Funct Ecol 18:, 670–676. [CrossRef]
    [Google Scholar]
  7. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Giménez-Jurado G., Kurtzman C. P., Starmer W. T., Spencer-Martins I.. ( 2003;). Metschnikowia vanudenii sp. nov. and Metschnikowia lachancei sp. nov., from flowers and associated insects in North America. . Int J Syst Evol Microbiol 53:, 1665–1670. [CrossRef][PubMed]
    [Google Scholar]
  9. Goldblatt P., Bernhardt P., Manning J. C.. ( 1998;). Pollination of petaloid geophytes by monkey beetles (Scarabaeidae: Rutelinae: Hopliini) in Southern Africa. . Ann Mo Bot Gard 85:, 215–230. [CrossRef]
    [Google Scholar]
  10. Guzmán B., Lachance M.-A., Herrera C. M.. ( 2013;). Phylogenetic analysis of the angiosperm-floricolous insect–yeast association: have yeast and angiosperm lineages co-diversified?. Mol Phylogenet Evol 68:, 161–175. [CrossRef][PubMed]
    [Google Scholar]
  11. Herrera C. M., García I. M., Pérez R.. ( 2008;). Invisible floral larcenies: microbial communities degrade floral nectar of bumble bee-pollinated plants. . Ecology 89:, 2369–2376. [CrossRef][PubMed]
    [Google Scholar]
  12. Hong S. G., Chun J., Oh H. W., Bae K. S.. ( 2001;). Metschnikowia koreensis sp. nov., a novel yeast species isolated from flowers in Korea. . Int J Syst Evol Microbiol 51:, 1927–1931. [CrossRef][PubMed]
    [Google Scholar]
  13. Kaewwichian R., Yongmanitchai W., Kawasaki H., Limtong S.. ( 2012;). Metschnikowia saccharicola sp. nov. and Metschnikowia lopburiensis sp. nov., two novel yeast species isolated from phylloplane in Thailand. . Antonie van Leeuwenhoek 102:, 743–751. [CrossRef][PubMed]
    [Google Scholar]
  14. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  15. Kurtzman C. P., Robnett C. J.. ( 1998;). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73:, 331–371. [CrossRef][PubMed]
    [Google Scholar]
  16. Kurtzman C. P., Fell J. W., Boekhout T., Robert V.. ( 2011;). Methods for the isolation, phenotypic characterization and maintenance of yeasts. . In The Yeasts: a Taxonomic Study, , 5th edn., vol. 1, pp. 87–110. Edited by Kurtzman C. P., Fell J. W., Boekhout T... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  17. Lachance M.-A.. ( 2011;). Metschnikowia Kamienski. . In The Yeasts: a Taxonomic Study, , 5th edn., vol. 1, pp. 575–620. Edited by Kurtzman C. P., Fell J. W., Boekhout T... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
  18. Lachance M.-A., Fedor A. N.. ( 2014;). Catching speciation in the act: Metschnikowia bowlesiae sp. nov., a yeast species found in nitidulid beetles of Hawaii and Belize. . Antonie van Leeuwenhoek 105:, 541–550. [CrossRef][PubMed]
    [Google Scholar]
  19. Lachance M.-A., Bowles J. M., Starmer W. T., Barker J. S. F.. ( 1999;). Kodamaea kakaduensis and Candida tolerans, two new ascomycetous yeast species from Australian Hibiscus flowers. . Can J Microbiol 45:, 172–177. [CrossRef][PubMed]
    [Google Scholar]
  20. Lachance M.-A., Starmer W. T., Rosa C. A., Bowles J. M., Barker J. S. F., Janzen D. H.. ( 2001;). Biogeography of the yeasts of ephemeral flowers and their insects. . FEMS Yeast Res 1:, 1–8. [CrossRef][PubMed]
    [Google Scholar]
  21. Lachance M.-A., Bowles J. M., Starmer W. T.. ( 2003a;). Metschnikowia santaceciliae, Candida hawaiiana, and Candida kipukae, three new yeast species associated with insects of tropical morning glory. . FEMS Yeast Res 3:, 97–103.[PubMed]
    [Google Scholar]
  22. Lachance M.-A., Bowles J. M., Starmer W. T.. ( 2003b;). Geography and niche occupancy as determinants of yeast biodiversity: the yeast–insect–morning glory ecosystem of Kīpuka Puaulu, Hawai’i. . FEMS Yeast Res 4:, 105–111. [CrossRef][PubMed]
    [Google Scholar]
  23. Lachance M.-A., Ewing C. P., Bowles J. M., Starmer W. T.. ( 2005;). Metschnikowia hamakuensis sp. nov., Metschnikowia kamakouana sp. nov. and Metschnikowia mauinuiana sp. nov., three endemic yeasts from Hawaiian nitidulid beetles. . Int J Syst Evol Microbiol 55:, 1369–1377. [CrossRef][PubMed]
    [Google Scholar]
  24. Lachance M.-A., Anderson T. M., Starmer W. T.. ( 2006a;). A new subclade of haplontic Metschnikowia species associated with insects of morning glory flowers in Africa and description of Metschnikowia aberdeeniae sp. nov.. Int J Syst Evol Microbiol 56:, 1141–1145. [CrossRef][PubMed]
    [Google Scholar]
  25. Lachance M.-A., Bowles J. M., Wiens F., Dobson J., Ewing C. P.. ( 2006b;). Metschnikowia orientalis sp. nov., an Australasian yeast from nitidulid beetles. . Int J Syst Evol Microbiol 56:, 2489–2493. [CrossRef][PubMed]
    [Google Scholar]
  26. Lachance M.-A., Bowles J. M., Anderson T. M., Starmer W. T.. ( 2008;). Metschnikowia shivogae sp. nov., a yeast species associated with insects of morning glory flowers in East Africa. . Int J Syst Evol Microbiol 58:, 2241–2244. [CrossRef][PubMed]
    [Google Scholar]
  27. Marinoni G., Lachance M.-A.. ( 2004;). Speciation in the large-spored Metschnikowia clade and establishment of a new species, Metschnikowia borealis comb. nov. . FEMS Yeast Res 4:, 587–596. [CrossRef][PubMed]
    [Google Scholar]
  28. Nguyen N. H., Suh S. O., Erbil C. K., Blackwell M.. ( 2006;). Metschnikowia noctiluminum sp. nov., Metschnikowia corniflorae sp. nov., and Candida chrysomelidarum sp. nov., isolated from green lacewings and beetles. . Mycol Res 110:, 346–356. [CrossRef][PubMed]
    [Google Scholar]
  29. Nicolson S. W.. ( 2007;). Nectar consumers. . In Nectaries and Nectar, pp. 289–342. Edited by Nicolson S. W., Nepi M., Pacini W... Dordrecht:: Springer;. [CrossRef]
    [Google Scholar]
  30. Picker M. D., Midgley J. J.. ( 1996;). Pollination by monkey beetles (Coleoptera, Scarabaeidae, Hopliini), flower and colour preferences. . Afr Entomol 4:, 7–14.
    [Google Scholar]
  31. Posada D.. ( 2008;). jModelTest: phylogenetic model averaging. . Mol Biol Evol 25:, 1253–1256. [CrossRef][PubMed]
    [Google Scholar]
  32. Pozo M. I., Herrera C. M., Bazaga P.. ( 2011;). Species richness of yeast communities in floral nectar of southern Spanish plants. . Microb Ecol 61:, 82–91. [CrossRef][PubMed]
    [Google Scholar]
  33. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  34. Simpson B. B., Neff J. L.. ( 1983;). Evolution and diversity of floral rewards. . In Handbook of Experimental Pollination Biology, pp. 142–159. Edited by Jones C. E., Little R. J... New York:: Van Nostrand Reinhold;.
    [Google Scholar]
  35. Steiner K. E.. ( 1998;). Beetle pollination of peacock moraeas (Iridaceae) in South Africa. . Plant Syst Evol 209:, 47–65. [CrossRef]
    [Google Scholar]
  36. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30:, 2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  37. Wallace I. M., O’Sullivan O., Higgins D. G., Notredame C.. ( 2006;). M-Coffee: combining multiple sequence alignment methods with T-Coffee. . Nucleic Acids Res 34:, 1692–1699. [CrossRef][PubMed]
    [Google Scholar]
  38. Wang S. A., Jia J. H., Bai F. Y.. ( 2008;). Candida alocasiicola sp. nov., Candida hainanensis sp. nov., Candida heveicola sp. nov. and Candida musiphila sp. nov., novel anamorphic, ascomycetous yeast species isolated from plants. . Antonie van Leeuwenhoek 94:, 257–265. [CrossRef][PubMed]
    [Google Scholar]
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