1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_2

sp. nov., isolated from sandy soil Free

Abstract

A Gram-stain-negative, non-motile, heterotrophic, rod-shaped bacterium, designated strain W402, was isolated from an enrichment culture of a rhizosphere soil sample from the Xinjiang desert in the PR China. Analysis of the almost-complete 16S rRNA gene sequence showed that the isolate was phylogenetically related to a species of the genus , having a close relationship to JA744 (97 % similarity). However, DNA–DNA relatedness between W402 and JA744 was 43.2±1.2 %. Strain W402 grew in 0–10 % (w/v) NaCl. The temperature and pH ranges for growth were 10–40 °C and pH 5.5–10.0, respectively. Optimal growth occurred at 1–3 % (w/v) NaCl, 30 °C and pH 7.0. The predominant cellular fatty acids were summed feature 8 (Cω7 and/or Cω6, 61.4 %), C (9.8 %) and 11-methyl Cω7 (8.2 %). The major quinone of strain W402 was Q-10. Phosphatidylethanolamine was predominant in the polar lipid profile. The DNA G+C content of strain W402 was 67.3 mol%. Taken together, these results confirm that W402 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W402 ( = ACCC 05851 = KCTC 32408).

  • Published Online:
Funding
This study was supported by the:
  • Ministry of Science and Technology of China (Award 2012AA02A703, 2012AA063503 and 2013CB733903, 2010CB126504)
  • Important National Science & Technology Specific Projects (Award 2013ZX08012-001, 2013ZX08009-003)
  • National Natural Science Foundation of China (Award 31170105, 30925002, 31230004)
  • Special Fund for Agro-scientific Research in the Public Interest (Award 201103007)
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.068262-0
2015-02-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/2/650.html?itemId=/content/journal/ijsem/10.1099/ijs.0.068262-0&mimeType=html&fmt=ahah

References

  1. Bibi F., Chung E. J., Khan A., Jeon C. O., Chung Y. R. ( 2013 ). Martelella endophytica sp. nov., an antifungal bacterium associated with a halophyte. . Int J Syst Evol Microbiol 63, 2914–2919. [View Article] [PubMed]
    [Google Scholar]
  2. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  3. Jiang S., Chen M., Su S., Yang M., Li A., Zhang C., Lin M., Zhang W., Luo X. ( 2014 ). Sphingobacterium arenae sp. nov., isolated from sandy soil. . Int J Syst Evol Microbiol 64, 248–253. [View Article] [PubMed]
    [Google Scholar]
  4. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 989–1005. [View Article]
    [Google Scholar]
  5. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  6. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  7. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 1596–1599. [View Article] [PubMed]
    [Google Scholar]
  8. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109–118. [View Article] [PubMed]
    [Google Scholar]
  9. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  10. Ormerod J. G., Ormerod K. S., Gest H. ( 1961 ). Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. . Arch Biochem Biophys 94, 449–463. [View Article] [PubMed]
    [Google Scholar]
  11. Owen R. J., Lapage S. P. ( 1976 ). The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxonomic applications. . J Appl Bacteriol 41, 335–340. [View Article] [PubMed]
    [Google Scholar]
  12. Park M., Ryu S. H., Vu T.-H. T., Ro H.-S., Yun P.-Y., Jeon C. O. ( 2007 ). Flavobacterium defluvii sp. nov., isolated from activated sludge. . Int J Syst Evol Microbiol 57, 233–237. [View Article] [PubMed]
    [Google Scholar]
  13. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  14. Skerman V. B. D. ( 1967 ). A Guide to the Identification of the Genera of Bacteria, , 2nd edn.. Baltimore, MD:: Williams & Wilkins;.
     [Google Scholar]
  15. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch. V. ( 2013 ). Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. . Int J Syst Evol Microbiol 63, 2132–2137. [View Article] [PubMed]
    [Google Scholar]
  16. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128–130. [View Article]
    [Google Scholar]
  17. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199–202. [View Article]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  19. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697–703.[PubMed]
    [Google Scholar]
  20. Yuan M., Zhang W., Dai S., Wu J., Wang Y., Tao T., Chen M., Lin M. ( 2009 ). Deinococcus gobiensis sp. nov., an extremely radiation-resistant bacterium. . Int J Syst Evol Microbiol 59, 1513–1517. [View Article] [PubMed]
    [Google Scholar]
  21. Zhang W., Zhu H. H., Yuan M., Yao Q., Tang R., Lin M., Yang S. Z., Li Z. K., Chen M. ( 2010 ). Microbacterium radiodurans sp. nov., a UV radiation-resistant bacterium isolated from soil. . Int J Syst Evol Microbiol 60, 2665–2670. [View Article] [PubMed]
    [Google Scholar]
  22. Zhou Z., Yuan M., Tang R., Chen M., Lin M., Zhang W. ( 2012 ). Corynebacterium deserti sp. nov., isolated from desert sand. . Int J Syst Evol Microbiol 62, 791–794. [View Article] [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.068262-0
Loading
Falsirhodobacter deserti sp. nov., isolated from sandy soil
Int J Syst Evol Microbiol 65, 650 (2015); https://doi.org/10.1099/ijs.0.068262-0
/content/journal/ijsem/10.1099/ijs.0.068262-0
/content/journal/ijsem/10.1099/ijs.0.068262-0
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error