1887

Abstract

A Gram-stain-negative, non-motile, heterotrophic, rod-shaped bacterium, designated strain W402, was isolated from an enrichment culture of a rhizosphere soil sample from the Xinjiang desert in the PR China. Analysis of the almost-complete 16S rRNA gene sequence showed that the isolate was phylogenetically related to a species of the genus , having a close relationship to JA744 (97 % similarity). However, DNA–DNA relatedness between W402 and JA744 was 43.2±1.2 %. Strain W402 grew in 0–10 % (w/v) NaCl. The temperature and pH ranges for growth were 10–40 °C and pH 5.5–10.0, respectively. Optimal growth occurred at 1–3 % (w/v) NaCl, 30 °C and pH 7.0. The predominant cellular fatty acids were summed feature 8 (Cω7 and/or Cω6, 61.4 %), C (9.8 %) and 11-methyl Cω7 (8.2 %). The major quinone of strain W402 was Q-10. Phosphatidylethanolamine was predominant in the polar lipid profile. The DNA G+C content of strain W402 was 67.3 mol%. Taken together, these results confirm that W402 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W402 ( = ACCC 05851 = KCTC 32408).

Funding
This study was supported by the:
  • Ministry of Science and Technology of China (Award 2012AA02A703, 2012AA063503 and 2013CB733903, 2010CB126504)
  • Important National Science & Technology Specific Projects (Award 2013ZX08012-001, 2013ZX08009-003)
  • National Natural Science Foundation of China (Award 31170105, 30925002, 31230004)
  • Special Fund for Agro-scientific Research in the Public Interest (Award 201103007)
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/content/journal/ijsem/10.1099/ijs.0.068262-0
2015-02-01
2021-04-22
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