1887

Abstract

A Gram-positive, rod-shaped, endospore-forming isolate, Bi. , recovered in Portugal from a health product was subjected to a polyphasic study and compared with the type strains of , , and , the phenotypically and genotypically most closely related species. Acid production from cellobiose, -glucose and -mannose and absence of acid production from -arabinose, erythritol, inositol, maltose, mannitol, raffinose, rhamnose, sorbitol, starch and -tryptophan discriminated this new isolate from the type strains of the most closely related species. Additionally, a significant different protein and carbohydrate signature was evidenced by spectroscopic techniques, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and Fourier transform IR spectroscopy with attenuated total reflectance. Using a chemometric approach, the score plot generated by principal component analysis clearly delineated the isolate as a separate cluster. The quinone system for strain Bi. comprised predominantly menaquinone MK-7 and major polar lipids were diphosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. Strain Bi. showed ≥99 % 16S rRNA gene sequence similarity to FO-036b, (7061 and SAFR-032), 41KF2b and HYC-10. Differences in strain Bi. and sequences in comparison with the most closely related species and DNA–DNA hybridization experiments with Bi. and ATCC 7061, FO-036b, 41KF2b and HYC-10 gave relatedness values of 39.6 % (reciprocal 38.0 %), 49.9 % (reciprocal 42.9 %), 61.9 % (reciprocal 52.2 %) and 61.7 % (reciprocal 49.2 %), respectively, supported the delineation of strain Bi. as a representative of a novel species of the genus , for which the name sp. nov. is proposed, with strain Bi. ( = DSM 26896 = CCUG 64113) as the type strain.

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2014-11-01
2019-11-18
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