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Volume 65, Issue Pt_2

gen. nov., sp. nov., a thermophilic, agar-degrading bacterium isolated from compost Free

Abstract

A thermophilic, agar-degrading bacterium, strain FAB2, was isolated from sewage sludge compost. According to phylogenetic analysis based on 16S rRNA gene sequences, strain FAB2 belonged to the family within the phylum . However, FAB2 was different enough at the genus level from closely related species. The percentages of 16S rRNA gene sequence similarity with related organisms were 90.4 % for , 91.8 % for , 89.4 % for , 90.1 % for , and 89.0 % for . Morphological and physiological analyses revealed that the strain was motile, rod-shaped, Gram-stain-positive, aerobic and able to form oval endospores in swollen sporangia. Ammonium was required as a nitrogen source while nitrate, nitrite, urea and glutamate were not utilized. Catalase and oxidase activities were weakly positive and positive, respectively. The bacterium grew in the temperature range of 50–65 °C and in media with pH 7.5 to 9.0. Optimal growth occurred at 60 °C and pH 8.0–8.6. Growth was inhibited at pH≤7.0 and NaCl concentrations ≥2.5 % (w/v). In chemotaxonomic characterization, MK-7 was identified as the dominant menaquinone. Major fatty acids were iso-C and C. Dominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phosphatidylcholine was present in a moderate amount. The diamino acid in the cell wall was -diaminopimelic acid. The G+C content of the genomic DNA was 49.5 mol% in a nucleic acid study. On the basis of genetic and phenotypic characteristics, strain FAB2 ( = NBRC 109510 = KCTC 33130) showed characteristics suitable for classification as the type strain of a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed.

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Funding
This study was supported by the:
  • JSPS KAKENHI (Award 20225775)
© 2015 IUMS
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2015-02-01
2024-04-27
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Ammoniibacillus agariperforans gen. nov., sp. nov., a thermophilic, agar-degrading bacterium isolated from compost
Int J Syst Evol Microbiol 65, 570 (2015); https://doi.org/10.1099/ijs.0.067843-0
/content/journal/ijsem/10.1099/ijs.0.067843-0
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