1887

Abstract

The morphology and morphogenesis of an oxytrichid ciliate, (Blatterer & Foissner, 1990) Berger, 1999, collected from brackish and marine waters in China, were investigated using live observation and the protargol staining method. The main features of the morphogenetic process are: (i) the parental adoral zone of membranelles is retained completely in the proter and the anlage of undulating membranes originates from dedifferentiation of the old structures; (ii) three frontal, four frontoventral, one buccal, five ventral and five transverse cirri are derived from the anlagen of the undulating membranes and the five streaks of frontal-ventral-transverse anlagen in the pattern of 1 : 3 : 3 : 3 : 4 : 4 from left to right; (iii) the morphogenesis of the dorsal kineties is simpler than the pattern, i.e. without fragmentation of the dorsal kinety 3 anlagen; (iv) the single caudal cirrus originates from the dorsal kinety 3 anlage on the right side; (v) the two macronuclear nodules fuse into a single mass during the mid-stage of morphogenesis. These features correspond well with , indicating that the morphogenetic pattern of is stable. Phylogenetic analysis based on small-subunit rRNA gene sequence data supports the monophyly of the genus , which is nested within the non-Stylonychinae clade.

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2015-01-01
2019-11-18
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References

  1. Berger H. . ( 1999; ). Monograph of the Oxytrichidae (Ciliophora, Hypotrichia) ( Monographiae Biologicae , vol. 78). Dordrecht:: Springer;. [CrossRef]
    [Google Scholar]
  2. Berger H. . ( 2006; ). Monograph of the Urostyloidea (Ciliophora, Hypotricha) ( Monographiae Biologicae , vol. 85). Dordrecht:: Springer;.
    [Google Scholar]
  3. Berger H. . ( 2008; ). Monograph of the Amphisiellidae and Trachelostylidae (Ciliophora, Hypotricha) ( Monographiae Biologicae , vol. 88). Dordrecht:: Springer;.
    [Google Scholar]
  4. Berger H. . ( 2011; ). Monograph of the Gonostomatidae and Kahliellidae (Ciliophora, Hypotricha) ( Monographiae Biologicae , vol. 90). Dordrecht:: Springer;. [CrossRef]
    [Google Scholar]
  5. Berger H. , Foissner W. . ( 1997; ). Cladistic relationships and generic characterization of oxytrichid hypotrichs (Protozoa, Ciliophora). . Arch Protistenkd 148:, 125–155. [CrossRef]
    [Google Scholar]
  6. Bernhard D. , Stechmann A. , Foissner W. , Ammermann D. , Hehn M. , Schlegel M. . ( 2001; ). Phylogenetic relationships within the class Spirotrichea (Ciliophora) inferred from small subunit rRNA gene sequences. . Mol Phylogenet Evol 21:, 86–92. [CrossRef] [PubMed]
    [Google Scholar]
  7. Blatterer H. , Foissner W. . ( 1990; ). Beiträge zur ciliatenfauna (Protozoa: Ciliophora) der Amper (Bayern, Bundesrepublik Deutschland). . Arch Protistenkd 138:, 93–115 (in German). [CrossRef]
    [Google Scholar]
  8. Chen Z. , Song W. . ( 2002; ). Comparison of molecular biological techniques (RAPD, ARDRA and SSrRNA gene sequencing) on the phylogenetic relationships of lower taxa within spirotrichous ciliates. . High Technol Lett 8:, 1–6.
    [Google Scholar]
  9. Chen X. , Miao M. , Ma H. , Shao C. , Al-Rasheid K. A. S. . ( 2013a; ). Morphology, morphogenesis and small-subunit rRNA gene sequence of the novel brackish-water ciliate Strongylidium orientale sp. nov. (Ciliophora, Hypotrichia). . Int J Syst Evol Microbiol 63:, 1155–1164. [CrossRef] [PubMed]
    [Google Scholar]
  10. Chen X. , Yan Y. , Hu X. , Zhu M. , Ma H. , Warren A. . ( 2013b; ). Morphology and morphogenesis of a soil ciliate, Rigidohymena candens (Kahl, 1932) Berger, 2011 (Ciliophora, Hypotricha, Oxytrichidae), with notes on its molecular phylogeny based on small-subunit rDNA sequence data. . Int J Syst Evol Microbiol 63:, 1912–1921. [CrossRef] [PubMed]
    [Google Scholar]
  11. Chen X. , Hu X. , Lin X. , Al-Rasheid K. A. S. , Ma H. , Miao M. . ( 2013c; ). Morphology, ontogeny and molecular phylogeny of a newbrackish water ciliate Bakuella subtropica sp. n. (Ciliophora, Hypotricha) from southern China. . Eur J Protistol 49:, 611–622. [CrossRef] [PubMed]
    [Google Scholar]
  12. Elwood H. J. , Olsen G. J. , Sogin M. L. . ( 1985; ). The small-subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustulata . . Mol Biol Evol 2:, 399–410.[PubMed]
    [Google Scholar]
  13. Fan Y. B. , Chen X. M. , Hu X. , Shao C. , Al-Rasheid K. A. S. , Al-Farraj S. A. , Lin X. . ( 2014; ). Morphology and morphogenesis of Apoholosticha sinica n. g., n. sp. (Ciliophora, Hypotrichia), with consideration of its systematic position among urostylids. . Eur J Protistol 50:, 78–88. [CrossRef] [PubMed]
    [Google Scholar]
  14. Foissner W. . ( 1996; ). Ontogenesis in ciliated protozoa, with emphasis on stomatogenesis. . In Ciliates, Cells as Organisms, pp. 95–177. Edited by Hausmann K. , Bradbury P. C. . . Stuttgart:: Gustav Fischer;.
    [Google Scholar]
  15. Foissner W. , Moon-van der Staay S. Y. , van der Staay G. W. M. , Hackstein J. H. P. , Krautgartner W.-D. , Berger H. . ( 2004; ). Reconciling classical and molecular phylogenies in the stichotrichines (Ciliophora, Spirotrichea), including new sequences from some rare species. . Eur J Protistol 40:, 265–281. [CrossRef]
    [Google Scholar]
  16. Gao F. , Katz L. A. . ( 2014; ). Phylogenomic analyses support the bifurcation of ciliates into two major clades that differ in properties of nuclear division. . Mol Phylogenet Evol 70:, 240–243. [CrossRef] [PubMed]
    [Google Scholar]
  17. Gao F. , Katz L. A. , Song W. . ( 2013; ). Multigene-based analyses on evolutionary phylogeny of two controversial ciliate orders: Pleuronematida and Loxocephalida (Protista, Ciliophora, Oligohymenophorea). . Mol Phylogenet Evol 68:, 55–63. [CrossRef] [PubMed]
    [Google Scholar]
  18. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  19. Huang J. , Chen Z. , Song W. , Berger H. . ( 2014; ). Three-gene based phylogeny of the Urostyloidea (Protista, Ciliophora, Hypotricha), with notes on classification of some core taxa. . Mol Phylogenet Evol 70:, 337–347. [CrossRef] [PubMed]
    [Google Scholar]
  20. Jiang J. , Huang J. , Li L. , Shao C. , Al-Rasheid K. A. , Al-Farraj S. A. , Chen Z. . ( 2013; ). Morphology, ontogeny, and molecular phylogeny of two novel bakuellid-like hypotrichs (Ciliophora: Hypotrichia), with establishment of two new genera. . Eur J Protistol 49:, 78–92. [CrossRef] [PubMed]
    [Google Scholar]
  21. Küppers G. , Claps M. . ( 2013; ). Hypotrichous ciliates (Protozoa: Ciliophora) from a temporary pond in Argentina, with redescription of Apoamphisiella hymenophora (Stokes, 1886) Berger, 1999. . Zootaxa 3626:, 55–76. [CrossRef]
    [Google Scholar]
  22. Küppers G. C. , Paiva T. S. , Borges B. N. , Harada M. L. , Garraza G.G. , Mataloni G. . ( 2011; ). An Antarctic hypotrichous ciliate, Parasterkiella thompsoni (Foissner) nov. gen., nov. comb., recorded in Argentinean peat-bogs: morphology, morphogenesis, and molecular phylogeny. . Eur J Protistol 47:, 103–123. [CrossRef] [PubMed]
    [Google Scholar]
  23. Medlin L. , Elwood H. J. , Stickel S. , Sogin M. L. . ( 1988; ). The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. . Gene 71:, 491–499. [CrossRef] [PubMed]
    [Google Scholar]
  24. Miller M. A. , Pfeiffer W. , Schwartz T. . ( 2010; ). Creating the CIPRES Science Gateway for inference of large phylogenetic trees. . In Proceedings of the Gateway Computing Environment Workshop (GCE), pp. 1–8, 14 November 2010, New Orleans;, LA, USA:.[CrossRef]
    [Google Scholar]
  25. Naqvi I. , Gupta R. , Borgohain P. , Sapra G. R. . ( 2006; ). Morphology and morphogenesis of Rubrioxytricha indica n. sp. (Ciliophora: Hypotrichida). . Acta Protozool 45:, 53–64.
    [Google Scholar]
  26. Nylander, J. A. A. (2004). MrModeltest version 2. Distributed by the author. Evolutionary Biology Centre, Uppsala University, Sweden.
  27. Page R. D. M. . ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. . Comput Appl Biosci 12:, 357–358.[PubMed]
    [Google Scholar]
  28. Pan Y. , Li J. M. , Li L. , Hu X. , Al-Rasheid K. A. S. , Warren A. . ( 2013; ). Ontogeny and molecular phylogeny of a new marine ciliate genus, Heterokeronopsis g. n. (Protozoa, Ciliophora, Hypotricha), with description of a new species. . Eur J Protistol 49:, 298–311. [CrossRef] [PubMed]
    [Google Scholar]
  29. Park K. M. , Jung J. H. , Min G. S. . ( 2013; ). Morphology, morphogenesis, and molecular phylogeny of Anteholosticha multicirrata n. sp. (Ciliophora, Spirotrichea) with a note on morphogenesis of A. pulchra (Kahl, 1932) Berger, 2003. . J Eukaryot Microbiol 60:, 564–577. [CrossRef] [PubMed]
    [Google Scholar]
  30. Ronquist F. , Huelsenbeck J. P. . ( 2003; ). MrBayes 3: Bayesian phylogenetic inference under mixed models. . Bioinformatics 19:, 1572–1574. [CrossRef] [PubMed]
    [Google Scholar]
  31. Schmidt S. L. , Bernhard D. , Schlegel M. , Foissner W. . ( 2007; ). Phylogeny of the Stichotrichia (Ciliophora; Spirotrichea) reconstructed with nuclear small subunit rRNA gene sequences: discrepancies and accordances with morphological data. . J Eukaryot Microbiol 54:, 201–209. [CrossRef] [PubMed]
    [Google Scholar]
  32. Shao C. , Song W. , Al-Rasheid K. A. S. , Berger H. . ( 2011; ). Redefinition and reassignment of the 18-cirri genera Hemigastrostyla, Oxytricha, Urosomoida, and Actinotricha (Ciliophora, Hypotricha), and description of one new genus and two new species. . Acta Protozool 50:, 263–287.
    [Google Scholar]
  33. Shao C. , Ding Y. , Al-Rasheid K. A. , Al-Farraj S. A. , Warren A. , Song W. . ( 2013; ). Establishment of a new hypotrichous genus, Heterotachysoma n. gen. and notes on the morphogenesis of Hemigastrostyla enigmatica (Ciliophora, Hypotrichia). . Eur J Protistol 49:, 93–105. [CrossRef] [PubMed]
    [Google Scholar]
  34. Shin M. K. , Kim W. . ( 1993; ). New records of three oxytrichid hypotrichs (Ciliophora: Hypotrichida: Oxytrichidae) from the Han river in Seoul, Korea. . Korean J Zool 36:, 223–230.
    [Google Scholar]
  35. Singh J. , Kamra K. . ( 2013; ). Paraurosomoida indiensis gen. nov., sp. nov., an oxytrichid (Ciliophora, Hypotricha) from Kyongnosla Alpine Sanctuary, including note on non-oxytrichid Dorsomarginalia. . Eur J Protistol 49:, 600–610. [CrossRef] [PubMed]
    [Google Scholar]
  36. Singh J. , Kamra K. , Sapra G. R. . ( 2013; ). Morphology, ontogenesis, and molecular phylogeny of an Indian population of Cyrtohymena (Cyrtohymenides) shii, including remarks on the subgenus. . Eur J Protistol 49:, 283–297. [CrossRef] [PubMed]
    [Google Scholar]
  37. Song W. , Warren A. , Hu X. . ( 2009; ). Free-living Ciliates in the Bohai and Yellow Seas. Beijing:: Science Press (in Chinese and English);.
    [Google Scholar]
  38. Song W. , Wilbert N. , Li L. , Zhang Q. . ( 2011; ). Re-evaluation on the diversity of the polyphyletic genus Metaurostylopsis (Ciliophora, Hypotricha): ontogenetic, morphologic, and molecular data suggest the establishment of a new genus Apourostylopsis n. g.. J Eukaryot Microbiol 58:, 11–21. [CrossRef] [PubMed]
    [Google Scholar]
  39. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  40. Wilbert N. . ( 1975; ). Eine verbesserte Technik der Protargolimprägnation für Ciliaten. . Mikrokosmos 64:, 171–179 (in German).
    [Google Scholar]
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