1887

Abstract

A nitrogen-fixing marine bacterium, designated strain Gal22, was isolated from mangrove roots of . Cells were Gram-stain-negative rods, motile with a single polar flagellum. Growth was observed at 4–42 °C, pH 5.5 to 10 and with 0–18 % (w/v) NaCl. Strain Gal22 was positive for catalase and oxidase. Q-8 was the predominant lipoquinone. The DNA G+C content was 57.0 mol%. Based on phylogenetic analysis of 16S rRNA gene, strain Gal22 belongs to the genus . The closely related strains were shown to be DSM 22012 and IMCC1877 with 99 % and 96 % 16S rRNA gene sequence similarity, respectively. DNA–DNA relatedness analysis indicated that strain Gal22 was different from DSM 22012. On the basis of genotypic, morphological and biochemical characteristics, a novel species, sp. nov. (type strain, Gal22 = DSM 27697 = CIP 110653), is proposed.

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2014-12-01
2019-11-12
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References

  1. Aburto-Oropeza O., Ezcurra E., Danemann G., Valdez V., Murray J., Sala E.. ( 2008;). Mangroves in the Gulf of California increase fishery yields. . Proc Natl Acad Sci U S A 105:, 10456–10459. [CrossRef][PubMed]
    [Google Scholar]
  2. Bashan Y., Puente M. E., Myrold D. D., Toledo G.. ( 1998;). In vitro transfer of fixed nitrogen from diazotrophic filamentous cyanobacteria to black mangrove seedlings. . FEMS Microbiol Ecol 26:, 165–170. [CrossRef]
    [Google Scholar]
  3. Baumann P., Bowditch R. D., Baumann L., Beaman B.. ( 1983;). Taxonomy of marine Pseudomonas species: P. stanieri sp. nov.; P. perfectomarina sp. nov., nom. rev.; P. nautica; and P. doudorofii. . Int J Syst Bacteriol 33:, 857–865. [CrossRef]
    [Google Scholar]
  4. Bowditch R. D., Baumann L., Baumann P.. ( 1984;). Description of Oceanospirillum kriegii sp. nov. and O. jannaschii sp. nov. and assignment of two species of Alteromonas to this genus as O. commune comb. nov. and O. vagum comb. nov.. Curr Microbiol 10:, 221–230. [CrossRef]
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef][PubMed]
    [Google Scholar]
  6. Chang H.-W., Nam Y.-D., Kwon H.-Y., Park J. R., Lee J.-S., Yoon J.-H., An K.-G., Bae J.-W.. ( 2007;). Marinobacterium halophilum sp. nov., a marine bacterium isolated from the Yellow Sea. . Int J Syst Evol Microbiol 57:, 77–80. [CrossRef][PubMed]
    [Google Scholar]
  7. Chimetto L. A., Cleenwerck I., Brocchi M., Willems A., De Vos P., Thompson F. L.. ( 2011;). Marinobacterium coralli sp. nov., isolated from mucus of coral (Mussismilia hispida). . Int J Syst Evol Microbiol 61:, 60–64. [CrossRef][PubMed]
    [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  9. Donato D. C., Kauffman J. B., Murdiyarso D., Kurnianto S., Stidham M., Kanninen M.. ( 2011;). Mangroves among the most carbon-rich forests in the tropics. . Nat Geosci 4:, 293–297. [CrossRef]
    [Google Scholar]
  10. Duke N. C., Meynecke J.-O., Dittmann S., Ellison A. M., Anger K., Berger U., Cannicci S., Diele K., Ewel K. C.. & other authors ( 2007;). A world without mangroves?. Science 317:, 41–42. [CrossRef][PubMed]
    [Google Scholar]
  11. Feller I. C., Lovelock C. E., Berger U., McKee K. L., Joye S. B., Ball M. C.. ( 2010;). Biocomplexity in mangrove ecosystems. . Annu Rev Mar Sci 2:, 395–417. [CrossRef][PubMed]
    [Google Scholar]
  12. González J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B.. ( 1997;). Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. . Int J Syst Bacteriol 47:, 369–376. [CrossRef][PubMed]
    [Google Scholar]
  13. Holguin G., Guzman M. A., Bashan Y.. ( 1992;). Two new nitrogen-fixing bacteria from the rhizosphere of mangrove trees: their isolation, identification and in vitro interaction with rhizosphere Staphylococcus sp.. FEMS Microbiol Ecol 101:, 207–216.
    [Google Scholar]
  14. Holguin G., Vazquez P., Bashan Y.. ( 2001;). The role of sediment microorganisms in the productivity, conservation, and rehabilitation of mangrove ecosystems: an overview. . Biol Fertil Soils 33:, 265–278. [CrossRef]
    [Google Scholar]
  15. Huo Y.-Y., Xu X.-W., Cao Y., Wang C.-S., Zhu X.-F., Oren A., Wu M.. ( 2009;). Marinobacterium nitratireducens sp. nov. and Marinobacterium sediminicola sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 59:, 1173–1178. [CrossRef][PubMed]
    [Google Scholar]
  16. Huss V. A. R., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  17. Jorgensen J. H., Turnidge J. D.. ( 2007;). Antibacterial susceptibility tests: dilution and disk diffusion methods. . In Manual of Clinical Microbiology, , 9th edn., pp. 1152–1172. Edited by Murray P. R., Baron E. J., Jorgensen J. H., Landry M. L., Pfaller M. A... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  18. Kim H., Choo Y.-J., Song J., Lee J.-S., Lee K. C., Cho J.-C.. ( 2007;). Marinobacterium litorale sp. nov. in the order Oceanospirillales. . Int J Syst Evol Microbiol 57:, 1659–1662. [CrossRef][PubMed]
    [Google Scholar]
  19. Kim Y.-G., Jin Y.-A., Hwang C. Y., Cho B. C.. ( 2008;). Marinobacterium rhizophilum sp. nov., isolated from the rhizosphere of the coastal tidal-flat plant Suaeda japonica. . Int J Syst Evol Microbiol 58:, 164–167. [CrossRef][PubMed]
    [Google Scholar]
  20. Kim H., Oh H.-M., Yang S.-J., Lee J.-S., Hong J.-S., Cho J.-C.. ( 2009a;). Marinobacterium marisflavi sp. nov., isolated from a costal seawater. . Curr Microbiol 58:, 511–515. [CrossRef][PubMed]
    [Google Scholar]
  21. Kim S.-J., Park S.-J., Yoon D.-N., Park B.-J., Choi B.-R., Lee D.-H., Roh Y., Rhee S.-K.. ( 2009b;). Marinobacterium maritimum sp. nov., a marine bacterium isolated from Arctic sediment. . Int J Syst Evol Microbiol 59:, 3030–3034. [CrossRef][PubMed]
    [Google Scholar]
  22. Kim J. M., Lee S. H., Jung J. Y., Jeon C. O.. ( 2010;). Marinobacterium lutimaris sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 60:, 1828–1831. [CrossRef][PubMed]
    [Google Scholar]
  23. Kovacs N.. ( 1956;). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178:, 703. [CrossRef][PubMed]
    [Google Scholar]
  24. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E.. ( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradorhizobium japonicum. . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  25. Lee R. Y., Joye S. B.. ( 2006;). Seasonal patterns of nitrogen fixation and denitrification in oceanic mangrove habitats. . Mar Ecol Prog Ser 307:, 127–141. [CrossRef]
    [Google Scholar]
  26. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. & other authors ( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  27. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  28. Miller L. T.. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  29. Ostle A. G., Holt J. G.. ( 1982;). Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. . Appl Environ Microbiol 44:, 238–241.[PubMed]
    [Google Scholar]
  30. Reef R., Feller I. C., Lovelock C. E.. ( 2010;). Nutrition of mangroves. . Tree Physiol 30:, 1148–1160. [CrossRef][PubMed]
    [Google Scholar]
  31. Satomi M., Kimura B., Hamada T., Harayama S., Fujii T.. ( 2002;). Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov., Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. nov. . Int J Syst Evol Microbiol 52:, 739–747. [CrossRef][PubMed]
    [Google Scholar]
  32. Seymour J. R., Ahmed T., Marcos, Stocker R.. ( 2008;). A microfluidic chemotaxis assay to study microbial behavior in diffusing nutrient patches. . Limnol Oceanogr Methods 6:, 477–488. [CrossRef]
    [Google Scholar]
  33. Stackebrandt E., Goebel B. M.. ( 1994;). A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  34. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  35. Tindall B. J.. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  36. Tindall B. J.. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  37. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P.. ( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60:, 249–266. [CrossRef][PubMed]
    [Google Scholar]
  38. Tomlinson P. B.. ( 1986;). The Botany of Mangroves. Cambridge:: Cambridge University Press;.
    [Google Scholar]
  39. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K.-H., Ludwig W., Glöckner F. O., Rosselló-Móra R.. ( 2008;). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31:, 241–250. [CrossRef][PubMed]
    [Google Scholar]
  40. Zhou A., Baidoo E., He Z., Mukhopadhyay A., Baumohl J. K., Benke P., Joachimiak M. P., Xie M., Song R.. & other authors ( 2013;). Characterization of NaCl tolerance in Desulfovibrio vulgaris Hildenborough through experimental evolution. . ISME J 7:, 1790–1802. [CrossRef][PubMed]
    [Google Scholar]
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