1887

Abstract

A strictly aerobic, light-yellow-coloured, stalked bacterium, designated strain ZFGT-14, was isolated from the root of Jacq. collected from Taibai Mountain in Shaanxi province, north-west China, and was subjected to a taxonomic study using a polyphasic approach. This novel isolate grew at 7–33 °C (optimum 25–28 °C) and pH 6.0–10.0 (optimum pH 7.0–8.0). Flexirubin-type pigments were not produced. Cells were Gram-stain-negative, rod-shaped and motile with a single polar flagellum. The predominant respiratory quinone was Q-10. The major cellular fatty acids were summed feature 8 (comprising Cω7/Cω6), C, C cyclo ω8 and summed feature 3 (comprising Cω7 and/or Cω6) and the major polar lipids were phosphatidylglycerol and glycolipids. The DNA GC content was 57.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZFGT-14 was most closely related to the genus and had low sequence similarity (95.0–95.9 %) with all species with validly published names within the genus . Based on the phenotypic, phylogenetic and genotypic data, strain ZFGT-14 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ZFGT-14 ( = CCTCC AB 2013012 = KCTC 32296 = LMG 27605).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.066605-0
2014-12-01
2019-11-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/12/3964.html?itemId=/content/journal/ijsem/10.1099/ijs.0.066605-0&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  2. Cleenwerck I., Vandemeulebroecke K., Janssens D., Swings J.. ( 2002;). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov.. Int J Syst Evol Microbiol 52:, 1551–1558. [CrossRef][PubMed]
    [Google Scholar]
  3. Doetsch R. N.. ( 1981;). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 21–33. Edited by Gerdhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  4. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. . [CrossRef]
    [Google Scholar]
  6. Kim B.-C., Poo H., Lee K. H., Kim M. N., Kwon O.-Y., Shin K.-S.. ( 2012a;). Mucilaginibacter angelicae sp. nov., isolated from the rhizosphere of Angelica polymorpha Maxim. . Int J Syst Evol Microbiol 62:, 55–60. [CrossRef][PubMed]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012b;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  8. Kim S., Gong G., Park T. H., Um Y.. ( 2013;). Asticcacaulis solisilvae sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 63:, 3829–3834. [CrossRef][PubMed]
    [Google Scholar]
  9. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  10. Lane D. J.. ( 1991;). 16S–23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 125–175. Edited by Stackebrandt E., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  11. Liu Z. P., Wang B. J., Liu S. J., Liu Y. H.. ( 2005;). Asticcacaulis taihuensis sp. nov., a novel stalked bacterium isolated from Taihu Lake, China. . Int J Syst Evol Microbiol 55:, 1239–1242. [CrossRef][PubMed]
    [Google Scholar]
  12. McCammon S. A., Bowman J. P.. ( 2000;). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov.. Int J Syst Evol Microbiol 50:, 1055–1063. [CrossRef][PubMed]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  14. Pate J. L., Porter J. S., Jordan T. L.. ( 1973;). Asticcacaulis biprosthecum sp.nov. Life cycle, morphology and cultural characteristics. . Antonie van Leeuwenhoek 39:, 569–583. [CrossRef][PubMed]
    [Google Scholar]
  15. Poindexter J. S.. ( 1964;). Biological properties and classification of the Caulobacter group. . Bacteriol Rev 28:, 231–295.[PubMed]
    [Google Scholar]
  16. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  17. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  18. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  19. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. . [CrossRef][PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  22. Tindall B. J.. ( 1990;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  23. Vasilyeva L. V., Omelchenko M. V., Berestovskaya Y. Y., Lysenko A. M., Abraham W. R., Dedysh S. N., Zavarzin G. A.. ( 2006;). Asticcacaulis benevestitus sp. nov., a psychrotolerant, dimorphic, prosthecate bacterium from tundra wetland soil. . Int J Syst Evol Microbiol 56:, 2083–2088. [CrossRef][PubMed]
    [Google Scholar]
  24. Wilson K.. ( 1987;). Preparation of genomic DNA from bacteria. . In Current Protocols in Molecular Biology, pp. 241–245. Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K... New York:: Greene Publishing and Wiley Interscience;.
    [Google Scholar]
  25. Xie C. H., Yokota A.. ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49:, 345–349. [CrossRef][PubMed]
    [Google Scholar]
  26. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef][PubMed]
    [Google Scholar]
  27. Zhang L., Wang Y., Wei L., Wang Y., Shen X., Li S.. ( 2013;). Taibaiella smilacinae gen. nov., sp. nov., an endophytic member of the family Chitinophagaceae isolated from the stem of Smilacina japonica, and emended description of Flavihumibacter petaseus. . Int J Syst Evol Microbiol 63:, 3769–3776. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.066605-0
Loading
/content/journal/ijsem/10.1099/ijs.0.066605-0
Loading

Data & Media loading...

Supplements

Supplementary Data 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error