1887

Abstract

A Gram-stain-positive, strictly aerobic, non-motile, coccoid bacterium, designated NCCP-154, was isolated from citrus leaf canker lesions and was subjected to a polyphasic taxonomic study. Strain NCCP-154 grew at 10–37 °C (optimum 30 °C) and at pH 7.0–8.0 (optimum pH 7.0). The novel strain exhibited tolerance of UV irradiation (>1000 J m). Based on 16S rRNA gene sequence analysis, strain NCCP-154 showed the highest similarity to CGMCC 1.7299 (98.8 %), and less than 94 % similarity to other closely related taxa. The chemotaxonomic data [major menaquinone, MK-8; cell-wall peptidoglycan type, A3β (Orn–Gly); major fatty acids, summed feature 3 (Cω7c/iso-C 2-OH; 35.3 %) followed by C (12.7 %), iso-Cω9 (9.2 %), Cω8 (7.4 %) and iso-C (6.9 %); major polar lipids made up of several unidentified phosphoglycolipids and glycolipids and an aminophospholipid, and mannose as the predominant whole-cell sugar] also supported the affiliation of strain NCCP-154 to the genus . The level of DNA–DNA relatedness between strain NCCP-154 and JCM 16679 was 63.3±3.7 %. The DNA G+C content of strain NCCP-154 was 70.0 mol%. Based on the phylogenetic analyses, DNA–DNA hybridization and physiological and biochemical characteristics, strain NCCP-154 can be differentiated from species with validly published names. Therefore, it represents a novel species of the genus . The name sp. nov. is proposed, with the type strain NCCP-154 ( = JCM 19024 = DSM 24791 = KCTC 13793).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.066555-0
2014-12-01
2019-11-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/12/4134.html?itemId=/content/journal/ijsem/10.1099/ijs.0.066555-0&mimeType=html&fmt=ahah

References

  1. Ahmed I., Yokota A., Fujiwara T.. ( 2007;). A novel highly boron tolerant bacterium, Bacillus boroniphilus sp. nov., isolated from soil, that requires boron for its growth. . Extremophiles 11:, 217–224. [CrossRef][PubMed]
    [Google Scholar]
  2. Ahmed I., Kudo T., Abbas S., Ehsan M., Iino T., Fujiwara T., Ohkuma M.. ( 2014a;). Cellulomonas pakistanensis sp. nov., a moderately halotolerant Actinobacteria. . Int J Syst Evol Microbiol 64:, 2305–2311. [CrossRef][PubMed]
    [Google Scholar]
  3. Ahmed I., Sin Y., Paek J., Ehsan M., Hayat R., Iqbal M., Chang Y. H.. ( 2014b;). Description of Lysinibacillus pakistanensis. . Int J Agric Biol 16:, 447–450.
    [Google Scholar]
  4. Asker D., Awad T. S., Beppu T., Ueda K.. ( 2009;). Deinococcus aquiradiocola sp. nov., isolated from a radioactive site in Japan. . Int J Syst Evol Microbiol 59:, 144–149. [CrossRef][PubMed]
    [Google Scholar]
  5. Asker D., Awad T. S., McLandsborough L., Beppu T., Ueda K.. ( 2011;). Deinococcus depolymerans sp. nov., a gamma- and UV-radiation-resistant bacterium, isolated from a naturally radioactive site. . Int J Syst Evol Microbiol 61:, 1448–1453. [CrossRef][PubMed]
    [Google Scholar]
  6. Brooks B. W., Murray R. G. E.. ( 1981;). Nomenclature for “Micrococcus radiodurans” and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. . Int J Syst Bacteriol 31:, 353–360. [CrossRef]
    [Google Scholar]
  7. Callegan R. P., Nobre M. F., McTernan P. M., Battista J. R., Navarro-González R., McKay C. P., da Costa M. S., Rainey F. A.. ( 2008;). Description of four novel psychrophilic, ionizing radiation-sensitive Deinococcus species from alpine environments. . Int J Syst Evol Microbiol 58:, 1252–1258. [CrossRef][PubMed]
    [Google Scholar]
  8. Cha S., Srinivasan S., Seo T., Kim M. K.. ( 2014;). Deinococcus soli sp. nov., a gamma-radiation-resistant bacterium isolated from rice field soil. . Curr Microbiol 68:, 777–783. [CrossRef][PubMed]
    [Google Scholar]
  9. Chen W., Wang B. J., Hong H. Z., Yang H., Liu S. J.. ( 2012;). Deinococcus reticulitermitis sp. nov., isolated from a termite gut. . Int J Syst Evol Microbiol 62:, 78–83. [CrossRef][PubMed]
    [Google Scholar]
  10. de Groot A., Chapon V., Servant P., Christen R., Saux M. F., Sommer S., Heulin T.. ( 2005;). Deinococcus deserti sp. nov., a gamma-radiation-tolerant bacterium isolated from the Sahara Desert. . Int J Syst Evol Microbiol 55:, 2441–2446. [CrossRef][PubMed]
    [Google Scholar]
  11. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  12. Felsenstein J.. ( 2005;). phylip (Phylogeny Inference Package), version 3.6. Distributed by the author. . Department of Genome Sciences, University of Washington;, Seattle, WA, USA:.
  13. Ferreira A. C., Nobre M. F., Rainey F. A., Silva M. T., Wait R., Burghardt J., Chung A. P., da Costa M. S.. ( 1997;). Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. . Int J Syst Bacteriol 47:, 939–947. [CrossRef][PubMed]
    [Google Scholar]
  14. Hirsch P., Gallikowski C. A., Siebert J., Peissl K., Kroppenstedt R., Schumann P., Stackebrandt E., Anderson R.. ( 2004;). Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica. . Syst Appl Microbiol 27:, 636–645. [CrossRef][PubMed]
    [Google Scholar]
  15. Im W. T., Jung H. M., Ten L. N., Kim M. K., Bora N., Goodfellow M., Lim S., Jung J., Lee S. T.. ( 2008;). Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge. . Int J Syst Evol Microbiol 58:, 2348–2353. [CrossRef][PubMed]
    [Google Scholar]
  16. Jacin H., Mishkin A. R.. ( 1965;). Separation of carbohydrates on borate-impregnated silica gel G plates. . J Chromatogr A 18:, 170–173. [CrossRef][PubMed]
    [Google Scholar]
  17. Kämpfer P., Lodders N., Huber B., Falsen E., Busse H.-J.. ( 2008;). Deinococcus aquatilis sp. nov., isolated from water. . Int J Syst Evol Microbiol 58:, 2803–2806. [CrossRef][PubMed]
    [Google Scholar]
  18. Kawamoto I., Oka T., Nara T.. ( 1981;). Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. . J Bacteriol 146:, 527–534.[PubMed]
    [Google Scholar]
  19. Kudo T.. ( 2001;). Phospholipids. . In Identification Manual of Bacteria: Molecular Genetics and Molecular Biological Methods, pp. 135–144. Edited by Suzuki K., Hiraishi A., Yokota A... Tokyo:: Springer;.
    [Google Scholar]
  20. Lai W.-A., Kämpfer P., Arun A. B., Shen F.-T., Huber B., Rekha P. D., Young C.-C.. ( 2006;). Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L.. Int J Syst Evol Microbiol 56:, 787–791. [CrossRef][PubMed]
    [Google Scholar]
  21. Lee J.-J., Lee H. J., Jang G. S., Yu J. M., Cha J. Y., Kim S. J., Lee E. B., Kim M. K.. ( 2013;). Deinococcus swuensis sp. nov., a gamma-radiation-resistant bacterium isolated from soil. . J Microbiol 51:, 305–311. [CrossRef][PubMed]
    [Google Scholar]
  22. LPSN ( 2014;). Genus Deinococcus. . In List of Prokaryotic Names with Standing in Nomenclature, accessed 13 September 2014. http://www.bacterio.net/deinococcus.html
    [Google Scholar]
  23. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef][PubMed]
    [Google Scholar]
  24. Mikami H., Ishida Y.. ( 1983;). Post-column fluorometric detection of reducing sugars in high performance liquid chromatography using arginine. . Bunseki Kagaku 32:, E207–E210. [CrossRef]
    [Google Scholar]
  25. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  26. Peng F., Zhang L., Luo X. S., Dai J., An H. L., Tang Y. L., Fang C. X.. ( 2009;). Deinococcus xinjiangensis sp. nov., isolated from desert soil. . Int J Syst Evol Microbiol 59:, 709–713. [CrossRef][PubMed]
    [Google Scholar]
  27. Rainey F. A., Ray K., Ferreira M., Gatz B. Z., Nobre M. F., Bagaley D., Rash B. A., Park M. J., Earl A. M.. & other authors ( 2005;). Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. . Appl Environ Microbiol 71:, 5225–5235. [CrossRef][PubMed]
    [Google Scholar]
  28. Roohi A., Ahmed I., Paek J., Sin Y., Abbas S., Jamil M., Chang Y. H.. ( 2014;). Bacillus pakistanensis sp. nov., a halotolerant bacterium isolated from salt mines of the Karak Area in Pakistan. . Antonie van Leeuwenhoek 105:, 1163–1172. [CrossRef][PubMed]
    [Google Scholar]
  29. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  30. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  31. Shashidhar R., Bandekar J. R.. ( 2006;). Deinococcus mumbaiensis sp. nov., a radiation-resistant pleomorphic bacterium isolated from Mumbai, India. . FEMS Microbiol Lett 254:, 275–280. [CrossRef][PubMed]
    [Google Scholar]
  32. Shashidhar R., Bandekar J. R.. ( 2009;). Deinococcus piscis sp. nov., a radiation-resistant bacterium isolated from a marine fish. . Int J Syst Evol Microbiol 59:, 2714–2717. [CrossRef][PubMed]
    [Google Scholar]
  33. Sorokin D. Y.. ( 2005;). Is there a limit for high-pH life?. Int J Syst Evol Microbiol 55:, 1405–1406. [CrossRef][PubMed]
    [Google Scholar]
  34. Srinivasan S., Kim M. K., Lim S., Joe M., Lee M.. ( 2012a;). Deinococcus daejeonensis sp. nov., isolated from sludge in a sewage disposal plant. . Int J Syst Evol Microbiol 62:, 1265–1270. [CrossRef][PubMed]
    [Google Scholar]
  35. Srinivasan S., Lee J. J., Lim S., Joe M., Kim M. K.. ( 2012b;). Deinococcus humi sp. nov., isolated from soil. . Int J Syst Evol Microbiol 62:, 2844–2850. [CrossRef][PubMed]
    [Google Scholar]
  36. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  37. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  38. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30:, 2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  39. Wang W., Mao J., Zhang Z. D., Tang Q. Y., Xie Y. Q., Zhu J., Zhang L. X., Liu Z. H., Shi Y. H., Goodfellow M.. ( 2010;). Deinococcus wulumuqiensis sp. nov., and Deinococcus xibeiensis sp. nov., isolated from radiation-polluted soil. . Int J Syst Evol Microbiol 60:, 2006–2010. [CrossRef][PubMed]
    [Google Scholar]
  40. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  41. Yang Y., Itoh T., Yokobori S., Itahashi S., Shimada H., Satoh K., Ohba H., Narumi I., Yamagishi A.. ( 2009;). Deinococcus aerius sp. nov., isolated from the high atmosphere. . Int J Syst Evol Microbiol 59:, 1862–1866. [CrossRef][PubMed]
    [Google Scholar]
  42. Yoo S. H., Weon H. Y., Kim S. J., Kim Y. S., Kim B. Y., Kwon S. W.. ( 2010;). Deinococcus aerolatus sp. nov. and Deinococcus aerophilus sp. nov., isolated from air samples. . Int J Syst Evol Microbiol 60:, 1191–1195. [CrossRef][PubMed]
    [Google Scholar]
  43. Yuan M., Zhang W., Dai S., Wu J., Wang Y., Tao T., Chen M., Lin M.. ( 2009;). Deinococcus gobiensis sp. nov., an extremely radiation-resistant bacterium. . Int J Syst Evol Microbiol 59:, 1513–1517. [CrossRef][PubMed]
    [Google Scholar]
  44. Zhang Y.-Q., Sun C.-H., Li W.-J., Yu L.-Y., Zhou J.-Q., Zhang Y.-Q., Xu L.-H., Jiang C.-L.. ( 2007;). Deinococcus yunweiensis sp. nov., a gamma- and UV-radiation-resistant bacterium from China. . Int J Syst Evol Microbiol 57:, 370–375. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.066555-0
Loading
/content/journal/ijsem/10.1099/ijs.0.066555-0
Loading

Data & Media loading...

Supplements

Supplementary Data 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error