1887

Abstract

A Gram-stain-positive, strictly aerobic, non-motile, coccoid bacterium, designated NCCP-154, was isolated from citrus leaf canker lesions and was subjected to a polyphasic taxonomic study. Strain NCCP-154 grew at 10–37 °C (optimum 30 °C) and at pH 7.0–8.0 (optimum pH 7.0). The novel strain exhibited tolerance of UV irradiation (>1000 J m). Based on 16S rRNA gene sequence analysis, strain NCCP-154 showed the highest similarity to CGMCC 1.7299 (98.8 %), and less than 94 % similarity to other closely related taxa. The chemotaxonomic data [major menaquinone, MK-8; cell-wall peptidoglycan type, A3β (Orn–Gly); major fatty acids, summed feature 3 (Cω7c/iso-C 2-OH; 35.3 %) followed by C (12.7 %), iso-Cω9 (9.2 %), Cω8 (7.4 %) and iso-C (6.9 %); major polar lipids made up of several unidentified phosphoglycolipids and glycolipids and an aminophospholipid, and mannose as the predominant whole-cell sugar] also supported the affiliation of strain NCCP-154 to the genus . The level of DNA–DNA relatedness between strain NCCP-154 and JCM 16679 was 63.3±3.7 %. The DNA G+C content of strain NCCP-154 was 70.0 mol%. Based on the phylogenetic analyses, DNA–DNA hybridization and physiological and biochemical characteristics, strain NCCP-154 can be differentiated from species with validly published names. Therefore, it represents a novel species of the genus . The name sp. nov. is proposed, with the type strain NCCP-154 ( = JCM 19024 = DSM 24791 = KCTC 13793).

Funding
This study was supported by the:
  • , PSDP , (Award Research for Agricultural Development Project (RADP))
  • , Pakistan Agricultural Research Council (PARC), Islamabad, Pakistan , (Award CS-55/RADP/PARC)
  • , Japan Society for Promotion of Science (JSPS)
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2014-12-01
2020-09-19
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