1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_12

sp. nov., an actinomycete isolated from soil Free

Abstract

A novel actinomycete, designated strain TRM 49590, was isolated from a soil sample from Lop Nur in Xinjiang Province, China. Strain TRM 49590 was aerobic, Gram-staining-positive, with an optimum NaCl concentration for growth of 1.5 % (w/v) and an optimum temperature for growth of 28–37 °C. The aerial mycelium was sparse, cylindrical and smooth-surfaced with irregular branches on ISP medium 4. The whole-cell sugars of strain TRM 49590 were ribose and glucose. The diagnostic diamino acid contained -diaminopimelic acid. The predominant menaquinones were MK-9(H) and MK-9(H), with MK-9(H) and MK-10(H) present in smaller amounts. The major fatty acids were iso-C, anteiso-C and anteiso-C. The GC content of the genomic DNA was 62.2 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TRM 49590 belongs to the genus with a sequence similarity of 97.16 % with the most closely related species . Based on these observations, strain TRM 49590 is proposed to represent a novel species of the genus for which the name sp. nov. is suggested. The type strain is TRM 49590 ( = CCTCC AA 2013018 = NRRL B59109).

  • Published Online:
Funding
This study was supported by the:
  • National High Technology Research and Development Program of China (Award 2012AA021705)
  • National Basic Research Program of China (Award 2010CB134505)
  • National Natural Science Foundation (Award 31060001)
Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin/College of Life Science, Tarim University, Alar, 84330, China
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.066357-0
2014-12-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/12/4179.html?itemId=/content/journal/ijsem/10.1099/ijs.0.066357-0&mimeType=html&fmt=ahah

References

  1. Bérdy J. ( 2005 ). Bioactive microbial metabolites. . J Antibiot (Tokyo) 58, 1–26. [View Article] [PubMed]
    [Google Scholar]
  2. Chun J. S., Goodfellow M. ( 1995 ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol 45, 240–245. [View Article] [PubMed]
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 2259–2261. [View Article] [PubMed]
    [Google Scholar]
  4. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
     [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [CrossRef]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  7. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  8. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. ( 1997 ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47, 1129–1133. [View Article] [PubMed]
    [Google Scholar]
  9. Guan T. W., Zhao K., Xia Z., Lü L. ( 2010 ). Comparison of isolation media for actinobacteria from different saline environments in Xinjiang, China. . Chin J Appl Environ Biol 16, 429–431. [View Article]
    [Google Scholar]
  10. Hasegawa T., Takizawa M., Tanida S. ( 1983 ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29, 319–322. [View Article]
    [Google Scholar]
  11. Hu H., Lin H. P., Xie Q. Y., Li L., Xie X. Q., Hong K. ( 2012 ). Streptomyces qinglanensis sp. nov., isolated from mangrove sediment. . Int J Syst Evol Microbiol 62, 596–600. [View Article] [PubMed]
    [Google Scholar]
  12. Kelly K. L. ( 1964 ). Inter-Society Color Council-National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
     [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  14. Lazzarini A., Cavaletti L., Toppo G., Marinelli F. ( 2000 ). Rare genera of actinomycetes as potential producers of new antibiotics. . Anton van Leeuwenhoek 78, 399–405. [View Article]
    [Google Scholar]
  15. Lee H. J., Whang K. S. ( 2014 ). Streptomyces graminilatus sp. nov., isolated from bamboo litter. . Int J Syst Evol Microbiol 64, 528–532. [CrossRef]
    [Google Scholar]
  16. Li W. J., Zhang Y. G., Zhang Y. Q., Tang S. K., Xu P., Xu L. H., Jiang C. L. ( 2005 ). Streptomyces sodiiphilus sp. nov., a novel alkaliphilic actinomycete. . Int J Syst Evol Microbiol 55, 1329–1333. [View Article] [PubMed]
    [Google Scholar]
  17. Liu C. X., Wang X., Zhao J., Liu Q., Wang L., Guan X., He H. , Xiang W. . ( 2013 ). Streptomyces harbinensis sp. nov., an endophytic, ikarugamycin-producing actinomycete isolated from soybean root [Glycine max (L.) Merr]. . Int J Syst Evol Microbiol 63, 3579–3584. [View Article] [PubMed]
    [Google Scholar]
  18. McCarthy A. J., Williams S. T. ( 1992 ). Actinomycetes as agents of biodegradation in the environment–a review. . Gene 115, 189–192. [View Article] [PubMed]
    [Google Scholar]
  19. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  21. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313–340. [View Article]
    [Google Scholar]
  22. Shirling E. B., Gottlieb D. ( 1968a ). Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study. . Int J Syst Bacteriol 18, 69–189. [View Article]
    [Google Scholar]
  23. Shirling E. B., Gottlieb D. ( 1968b ). Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. . Int J Syst Bacteriol 18, 279–392. [View Article]
    [Google Scholar]
  24. Shirling E. B., Gottlieb D. ( 1969 ). Cooperative description of type cultures of Streptomyces. IV. Species descriptions from the second, third and fourth studies. . Int J Syst Bacteriol 19, 391–512. [View Article]
    [Google Scholar]
  25. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226–231.[PubMed]
    [Google Scholar]
  26. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  28. Waksman S. A., Henrici A. T. ( 1943 ). The nomenclature and classification of the actinomycetes. . J Bacteriol 46, 337–341.[PubMed]
    [Google Scholar]
  29. Williams S. T., Goodfellow M., Alderson G. ( 1989 ). Genus Streptomyces Waksman and Henrici 1943, 339AL . . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams S. T., Sharpe M. E., Holt J. G. . Baltimore:: Williams & Wilkins;.
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.066357-0
Loading
Streptomyces lopnurensis sp. nov., an actinomycete isolated from soil
Int J Syst Evol Microbiol 64, 4179 (2014); https://doi.org/10.1099/ijs.0.066357-0
/content/journal/ijsem/10.1099/ijs.0.066357-0
/content/journal/ijsem/10.1099/ijs.0.066357-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error