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Abstract

A novel actinomycete, designated strain TRM 49590, was isolated from a soil sample from Lop Nur in Xinjiang Province, China. Strain TRM 49590 was aerobic, Gram-staining-positive, with an optimum NaCl concentration for growth of 1.5 % (w/v) and an optimum temperature for growth of 28–37 °C. The aerial mycelium was sparse, cylindrical and smooth-surfaced with irregular branches on ISP medium 4. The whole-cell sugars of strain TRM 49590 were ribose and glucose. The diagnostic diamino acid contained -diaminopimelic acid. The predominant menaquinones were MK-9(H) and MK-9(H), with MK-9(H) and MK-10(H) present in smaller amounts. The major fatty acids were iso-C, anteiso-C and anteiso-C. The GC content of the genomic DNA was 62.2 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TRM 49590 belongs to the genus with a sequence similarity of 97.16 % with the most closely related species . Based on these observations, strain TRM 49590 is proposed to represent a novel species of the genus for which the name sp. nov. is suggested. The type strain is TRM 49590 ( = CCTCC AA 2013018 = NRRL B59109).

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2014-12-01
2019-11-21
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