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Abstract

A novel Gram-staining-negative, non-motile and rod-shaped bacterial strain containing flexirubin-type pigments, designated S31, was isolated from bank-side soil of the Xixi wetland in Zhejiang province, China. Growth occurred at 10–37 °C (optimum, 32 °C), pH 6–8 (optimum, pH 7) and with 0–2 % (w/v) NaCl (optimum, 1 %). Strain S31 shared highest 16S rRNA gene sequence similarities with H1 (96.2 %) and DW3 (96.4 %). Phylogenetic analysis suggested that strain S31 was a member of the genus . The dominant respiratory quinone was MK-6 and the DNA G+C content was 33.3 mol%. The major fatty acids were iso-C, summed feature 3 (iso-C 2-OH and/or Cω7) and anteiso-C. The major polar lipids of strain S31 were phosphatidylethanolamine, three unidentified aminolipids and four unidentified polar lipids. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain S31 represents a novel species of the genus , for which the name sp. nov. (type strain S31 = CGMCC 1.12802 = NBRC 110387) is proposed.

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 21077090 and 21137003)
  • , SRF for ROCS , (Award ZEM Z201010001 and SEM J20120548)
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2014-12-01
2021-03-09
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References

  1. Dong X. Z., Cai M. Y. ( 2001 ). Common Manual of Systematic Bacteriology. Beijing:: Science Press;.
    [Google Scholar]
  2. Fautz E., Reichenbach H. ( 1980 ). A simple test for flexirubin-type pigments. . FEMS Microbiol Lett 8, 8791. [CrossRef]
    [Google Scholar]
  3. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  5. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  6. Herzog P., Winkler I., Wolking D., Kämpfer P., Lipski A. ( 2008 ). Chryseobacterium ureilyticum sp. nov., Chryseobacterium gambrini sp. nov., Chryseobacterium pallidum sp. nov. and Chryseobacterium molle sp. nov., isolated from beer-bottling plants. . Int J Syst Evol Microbiol 58, 2633. [CrossRef] [PubMed]
    [Google Scholar]
  7. Huang Y. L., Ki J. S., Case R., Qian P. Y. ( 2008 ). Diversity and acylhomoserine lactone production among subtidal biofilm forming bacteria. . Aquat Microb Ecol 52, 185193. [CrossRef]
    [Google Scholar]
  8. Huo Y. Y., Xu X. W., Cui H. L., Wu M. ( 2010 ). Gracilibacillus ureilyticus sp. nov., a halotolerant bacterium from a saline-alkaline soil. . Int J Syst Evol Microbiol 60, 13831386. [CrossRef] [PubMed]
    [Google Scholar]
  9. Jeon Y. S., Lee K., Park S. C., Kim B. S., Cho Y. J., Ha S. M., Chun J. ( 2014 ). EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. . Int J Syst Evol Microbiol 64, 689691. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K. ( 1987 ). Lipids and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [CrossRef]
    [Google Scholar]
  13. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358361. [CrossRef]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  15. O’Sullivan L. A., Rinna J., Humphreys G., Weightman A. J., Fry J. C. ( 2006 ). Culturable phylogenetic diversity of the phylum ‘Bacteroidetes’ from river epilithon and coastal water and description of novel members of the family Flavobacteriaceae: Epilithonimonas tenax gen. nov., sp. nov. and Persicivirga xylanidelens gen. nov., sp. nov.. Int J Syst Evol Microbiol 56, 169180. [CrossRef] [PubMed]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  17. Shakéd T., Hantsis-Zacharov E., Halpern M. ( 2010 ). Epilithonimonas lactis sp. nov., isolated from raw cow’s milk. . Int J Syst Evol Microbiol 60, 675679. [CrossRef] [PubMed]
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
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