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Abstract

A novel thermophilic bacterial strain, CBS-Z, was isolated from a terrestrial hot spring in the Changbai Mountains, PR China. Cells of strain CBS-Z were short straight rods without flagella and had Gram-positive cell walls. Growth was observed at 40–90 °C (optimum 75 °C) and at pH 5.6–8.6 (optimum pH 7.8). The primary end-products from the fermentation of filter paper by strain CBS-Z were acetate, lactate, H, and CO. The main cellular fatty acids were iso-C, iso-C 3-OH and C. The G+C content of the genomic DNA was 36.08 mol%. Multiple sequence alignment of the 16S rRNA gene sequence and phylogenetic analyses indicated that strain CBS-Z belongs to the genus and the most similar micro-organism was DSM 8903 (96.36 % 16S rRNA gene sequence similarity); the 16S rRNA gene sequence similarity of strain CBS-Z to other species was below 95 %. Based on its phylogenetic and phenotypic characteristics, strain CBS-Z represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CBS-Z ( = DSM 26941 = CGMCC 1.5180).

Funding
This study was supported by the:
  • , National Basic Research Program of China , (Award 2012CB721003)
  • , 973 Program
  • , Natural Science Foundation , (Award 20100563 and 30970634)
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2015-01-01
2021-01-23
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References

  1. Blumer-Schuette S. E., Lewis D. L., Kelly R. M. ( 2010 ). Phylogenetic, microbiological, and glycoside hydrolase diversities within the extremely thermophilic, plant biomass-degrading genus Caldicellulosiruptor . . Appl Environ Microbiol 76, 80848092. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bonch-Osmolovskaya E. A., Sokolova T. G., Kostrikina N. A., Zavarzin G. A. ( 1990 ). Desulfurella acetivorans gen. nov. and sp. nov. – a new thermophilic sulfur-reducing eubacterium. . Arch Microbiol 153, 151155. [CrossRef]
    [Google Scholar]
  3. Bredholt S., Sonne-Hansen J., Nielsen P., Mathrani I. M., Ahring B. K. ( 1999 ). Caldicellulosiruptor kristjanssonii sp. nov., a cellulolytic, extremely thermophilic, anaerobic bacterium. . Int J Syst Bacteriol 49, 991996. [CrossRef] [PubMed]
    [Google Scholar]
  4. Hamilton-Brehm S. D., Mosher J. J., Vishnivetskaya T., Podar M., Carroll S., Allman S., Phelps T. J., Keller M., Elkins J. G. ( 2010 ). Caldicellulosiruptor obsidiansis sp. nov., an anaerobic, extremely thermophilic, cellulolytic bacterium isolated from Obsidian Pool, Yellowstone National Park. . Appl Environ Microbiol 76, 10141020. [CrossRef] [PubMed]
    [Google Scholar]
  5. Huang C.-Y., Patel B. K., Mah R. A., Baresi L. ( 1998 ). Caldicellulosiruptor owensensis sp. nov., an anaerobic, extremely thermophilic, xylanolytic bacterium. . Int J Syst Bacteriol 48, 9197. [CrossRef] [PubMed]
    [Google Scholar]
  6. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., Yi H., Won S., Chun J. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef]
    [Google Scholar]
  7. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  8. Miroshnichenko M. L., Kublanov I. V., Kostrikina N. A., Tourova T. P., Kolganova T. V., Birkeland N. K., Bonch-Osmolovskaya E. A. ( 2008 ). Caldicellulosiruptor kronotskyensis sp. nov. and Caldicellulosiruptor hydrothermalis sp. nov., two extremely thermophilic, cellulolytic, anaerobic bacteria from Kamchatka thermal springs. . Int J Syst Evol Microbiol 58, 14921496. [CrossRef] [PubMed]
    [Google Scholar]
  9. Mladenovska Z., Mathrani I. M., Ahring B. K. ( 1995 ). Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacterium. . Arch Microbiol 163, 223230. [CrossRef]
    [Google Scholar]
  10. Onyenwoke R. U., Lee Y. J., Dabrowski S., Ahring B. K., Wiegel J. ( 2006 ). Reclassification of Thermoanaerobium acetigenum as Caldicellulosiruptor acetigenus comb. nov. and emendation of the genus description. . Int J Syst Evol Microbiol 56, 13911395. [CrossRef] [PubMed]
    [Google Scholar]
  11. Rainey F. A., Donnison A. M., Janssen P. H., Saul D., Rodrigo A., Bergquist P. L., Daniel R. M., Stackebrandt E., Morgan H. W. ( 1994 ). Description of Caldicellulosiruptor saccharolyticus gen. nov., sp. nov: an obligately anaerobic, extremely thermophilic, cellulolytic bacterium. . FEMS Microbiol Lett 120, 263266. [CrossRef] [PubMed]
    [Google Scholar]
  12. Rainey F. A., Ward-Rainey N. L., Janssen P. H., Hippe H., Stackebrandt E. ( 1996 ). Clostridium paradoxum DSM 7308T contains multiple 16S rRNA genes with heterogeneous intervening sequences. . Microbiology 142, 20872095. [CrossRef] [PubMed]
    [Google Scholar]
  13. Rittmann S., Herwig C. ( 2012 ). A comprehensive and quantitative review of dark fermentative biohydrogen production. . Microb Cell Fact 11, 115. [CrossRef] [PubMed]
    [Google Scholar]
  14. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  15. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  16. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [CrossRef] [PubMed]
    [Google Scholar]
  17. Willquist K., Zeidan A. A., van Niel E. W. ( 2010 ). Physiological characteristics of the extreme thermophile Caldicellulosiruptor saccharolyticus: an efficient hydrogen cell factory. . Microb Cell Fact 9, 89. [CrossRef] [PubMed]
    [Google Scholar]
  18. Yang S. J., Kataeva I., Wiegel J., Yin Y., Dam P., Xu Y., Westpheling J., Adams M. W. ( 2010 ). Classification of ‘Anaerocellum thermophilum’ strain DSM 6725 as Caldicellulosiruptor bescii sp. nov.. Int J Syst Evol Microbiol 60, 20112015. [CrossRef] [PubMed]
    [Google Scholar]
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