1887

Abstract

A Gram-stain-positive, aerobic, motile by gliding, rod-shaped bacterial strain, THG-GM18, was isolated from soil of a bamboo grove. Strain THG-GM18 was able to grow in the presence of up to 6.0 % (w/v) NaCl, at 4–37 °C and at pH 7.0–10.0 in R2A medium. Based on 16S rRNA gene sequence similarity, strain THG-GM18 was closely related to species of the genus . The most closely related strains to strain THG-GM18 are CCM 1646 (98.5 % similarity), G2-1 (98.4 %), DSM 420 (98.2 %), DSM 20116 (98.1 %) and A6 (98.0 %). Strain THG-GM18 possessed chemotaxonomic properties consistent with those of members of the genus , such as peptidoglycan type A3α (-Lys–-Ala–-Thr–-Ala), MK-9 as major menaquinone and anteiso- and iso-branched compounds (anteiso-C, iso-C and anteiso-C) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, unidentified phospholipids, unidentified aminolipids, an unidentified glycolipid and unidentified lipids. The G+C content of the genomic DNA was 61.0 mol%. The DNA–DNA relatedness values between strain THG-GM18 and its closest phylogenetic neighbours were below 26.0 %. The results of physiological and biochemical tests allowed the differentiation of strain THG-GM18 from species of the genus with validly published names. sp. nov. is the proposed name, and the type strain is THG-GM18 ( = KACC 17531 = JCM 19335).

Funding
This study was supported by the:
  • , Kyung Hee University , (Award 20120115)
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2014-09-01
2021-01-20
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