1887

Abstract

The taxonomic position of a novel actinomycete isolated from soil in Fars Province (Iran) was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolate matched those described for members of the genus . On ISP2 medium, strain HM 1154 produced a dark cream, branched substrate mycelium and aerial hyphae that in some images also appeared spiral and that developed into greyish-white spore chains with a smooth surface. The isolate showed optimal growth at 28 °C and pH 6–9 with 0–4 % (w/v) NaCl. Whole-cell hydrolysates contained -diaminopimelic acid as diagnostic diamino acid, ribose and glucose. The main phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, three unknown phospholipids and an unknown aminophospholipid; MK-9(H) and MK-9(H) were the predominant menaquinones. The major cellular fatty acids were the branched saturated iso-C and anteiso-C. Strain HM 1154 exhibited the highest 16S rRNA gene sequence similarities to DSM 40146 (99.4 %), DSM 40346 (99.3 %), DSM 41920 (99.2 %), DSM 40831 (99.2 %), DSM 40260 (99.1 %), DSM 40509 (99.1 %), DSM 40027 (99.1 %), DSM 40029 (99.0 %) and DSM 40037 (99.0 %). DNA–DNA hybridization studies showed relatedness values of 11.0–35.8 % with the closest related species. Based on these results, strain HM 1154 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is HM 1154 ( = DSM 42018 = UTMC 1154 = CECT 8305).

Funding
This study was supported by the:
  • Biotechnology Development Council of Iran
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2014-10-01
2021-04-10
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