1887

Abstract

The taxonomic position of a novel actinomycete isolated from soil in Fars Province (Iran) was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolate matched those described for members of the genus . On ISP2 medium, strain HM 1154 produced a dark cream, branched substrate mycelium and aerial hyphae that in some images also appeared spiral and that developed into greyish-white spore chains with a smooth surface. The isolate showed optimal growth at 28 °C and pH 6–9 with 0–4 % (w/v) NaCl. Whole-cell hydrolysates contained -diaminopimelic acid as diagnostic diamino acid, ribose and glucose. The main phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, three unknown phospholipids and an unknown aminophospholipid; MK-9(H) and MK-9(H) were the predominant menaquinones. The major cellular fatty acids were the branched saturated iso-C and anteiso-C. Strain HM 1154 exhibited the highest 16S rRNA gene sequence similarities to DSM 40146 (99.4 %), DSM 40346 (99.3 %), DSM 41920 (99.2 %), DSM 40831 (99.2 %), subsp. DSM 40260 (99.1 %), DSM 40509 (99.1 %), DSM 40027 (99.1 %), DSM 40029 (99.0 %) and DSM 40037 (99.0 %). DNA–DNA hybridization studies showed relatedness values of 11.0–35.8 % with the closest related species. Based on these results, strain HM 1154 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is HM 1154 ( = DSM 42018 = UTMC 1154 = CECT 8305).

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2014-10-01
2019-11-12
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References

  1. Bouizgarne B. , Lanoot B. , Loqman S. , Spröer C. , Klenk H.-P. , Swings J. , Ouhdouch Y. . ( 2009; ). Streptomyces marokkonensis sp. nov., isolated from rhizosphere soil of Argania spinosa L.. Int J Syst Evol Microbiol 59:, 2857–2863. [CrossRef] [PubMed]
    [Google Scholar]
  2. Cashion P. , Holder-Franklin M. A. , McCully J. , Franklin M. . ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef] [PubMed]
    [Google Scholar]
  3. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  4. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  5. Hamedi J. , Mohammadipanah F. , Klenk H.-P. , Pötter G. , Schumann P. , Spröer C. , von Jan M. , Kroppenstedt R. M. . ( 2010; ). Streptomyces iranensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 60:, 1504–1509. [CrossRef] [PubMed]
    [Google Scholar]
  6. Hamdali H. , Virolle M. J. , von Jan M. , Spröer C. , Klenk H.-P. . ( 2011; ). Streptomyces youssoufiensis sp. nov., isolated from a Moroccan phosphate mine. . Int. J. Syst Evol Microbiol 61:, 1104–1108.[CrossRef]
    [Google Scholar]
  7. Huss V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kämpfer P. . ( 2012; ). Order XIV. Streptomycetales ord. nov.. In Bergey's Manual of Systematic Bacteriology, vol. 5, , 2nd edn., pp 1446–1767. Edited by Whitman W. B. , Parte A. , Goodfellow M. , Kämpfer P. , Busse H.-J. , Trujillo M. E. , Ludwig W. , Suzuki K. . . New York:: Springer;.
    [Google Scholar]
  9. Kawato N. , Shinobu R. . ( 1959; ). On Streptomyces herbaricolor sp. nov., Supplement: a single technique for microscopical observation. . Mem Osaka Univ Lib Arts Edu B Nat Sci 8:, 114–119.
    [Google Scholar]
  10. Kelly K. L. . ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  11. Kim B. Y. , Zucchi T. D. , Fiedler H. P. , Goodfellow M. . ( 2012; ). Streptomyces cocklensis sp. nov., a dioxamycin-producing actinomycete. . Int J Syst Evol Microbiol 62:, 279–283. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kontro M. , Lignell U. , Hirvonen M.-R. , Nevalainen A. . ( 2005; ). pH effects on 10 Streptomyces spp. growth and sporulation depend on nutrients. . Lett Appl Microbiol 41:, 32–38. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kroppenstedt R. M. . ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  14. Kroppenstedt R. M. . ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics, pp. 173–179. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  15. Kroppenstedt R. M. , Goodfellow M. . ( 2006; ). The family Thermomonosporaceae: Actinocorallia, Actinomadura, Spirillospora and Thermomonospora . . In The Prokaryotes, , 3rd edn., vol. 3, pp. 682–724. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K. H. , Stackebrandt E. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  16. Laatsch, H. ( 2011; ). AntiBase 2011: a database for rapid dereplication and structure determination of microbial natural products . Weinheim:: Wiley-VCH;. http://wwwuser.gwdg.de/~hlaatsc/antibase.htm .
  17. Lechevalier M. P. , Lechevalier H. A. . ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  18. Li W. , Lanoot B. , Zhang Y. , Vancanneyt M. , Swings J. , Liu Z. . ( 2002; ). Streptomyces scopiformis sp. nov., a novel streptomycete with fastigiate spore chains. . Int. J. Syst. Evol. Microbiol. 52:, 1629–1633.[CrossRef]
    [Google Scholar]
  19. Liu Z. , Zhao X. , Bai F. . ( 2013; ). Production of xylanase by an alkaline-tolerant marine-derived Streptomyces viridochromogenes strain and improvement by ribosome engineering. . Appl Microbiol Biotechnol 97:, 4361–4368. [CrossRef] [PubMed]
    [Google Scholar]
  20. Meier-Kolthoff J. P. , Göker M. , Spröer C. , Klenk H. P. . ( 2013; ). When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195:, 413–418. [CrossRef] [PubMed]
    [Google Scholar]
  21. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal K. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  22. Mohammadipanah F. , Matasyoh J. , Hamedi J. , Klenk H. P. , Laatsch H. . ( 2012; ). Persipeptides A and B, two cyclic peptides from Streptomyces sp. UTMC 1154. . Bioorg Med Chem 20:, 335–339. [CrossRef] [PubMed]
    [Google Scholar]
  23. Montero-Calasanz M. C. , Göker M. , Pötter G. , Rohde M. , Spröer C. , Schumann P. , Gorbushina A. A. , Klenk H. P. . ( 2013; ). Geodermatophilus africanus sp. nov., a halotolerant actinomycete isolated from Saharan desert sand. . Antonie van Leeuwenhoek 104:, 207–216. [CrossRef] [PubMed]
    [Google Scholar]
  24. Nolan R. D. , Cross T. . ( 1988; ). Isolation and screening of Actinomycetes. . In Actinomycetes in Biotechnology, pp. 1–8. Edited by Goodfellow M. , Williams S. T. , Mordarski M. . . London:: Academic Press;. [CrossRef]
    [Google Scholar]
  25. Nonomura H. , Ohara Y. . ( 1969; ). Distribution of actinomycetes in soil. VI. A culture method effective for both preferential isolation and enumeration of Microbispora and Streptosporangium strains in soil. . J Ferment Technol 47:, 463–469.
    [Google Scholar]
  26. Nonomura H. , Ohara Y. . ( 1971; ). Distribution of actinomycetes in soil. X. New genus and species of monosporic actinomycetes in soil. . J Ferment Technol 49:, 895–903.
    [Google Scholar]
  27. Pridham T. G. , Hesseltine C. W. , Benedict R. G. . ( 1958; ). A guide for the classification of streptomycetes according to selected groups; placement of strains in morphological sections. . Appl Microbiol 6:, 52–79.[PubMed]
    [Google Scholar]
  28. Rainey F. A. , Ward-Rainey N. , Kroppenstedt R. M. , Stackebrandt E. . ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:, 1088–1092. [CrossRef] [PubMed]
    [Google Scholar]
  29. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
  30. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  31. Schleissner C. , Pérez M. , Losada A. , Rodríguez P. , Crespo C. , Zúñiga P. , Fernández R. , Reyes F. , de la Calle F. . ( 2011; ). Antitumor actinopyranones produced by Streptomyces albus POR-04-15-053 isolated from a marine sediment. . J Nat Prod 74:, 1590–1596. [CrossRef] [PubMed]
    [Google Scholar]
  32. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  33. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  34. Vaas L. A. I. , Sikorski J. , Michael V. , Göker M. , Klenk H. P. . ( 2012; ). Visualization and curve-parameter estimation strategies for efficient exploration of phenotype microarray kinetics. . PLoS ONE 7:, e34846. [CrossRef] [PubMed]
    [Google Scholar]
  35. Vaas L. A. I. , Sikorski J. , Hofner B. , Fiebig A. , Buddruhs N. , Klenk H.-P. , Göker M. . ( 2013; ). opm: an R package for analysing OmniLog(R) phenotype microarray data. . Bioinformatics 29:, 1823–1824. [CrossRef] [PubMed]
    [Google Scholar]
  36. Waksman S. A. , Henrici A. T. . ( 1943; ). The nomenclature and classification of the Actinomycetes. . J Bacteriol 46:, 337–341.[PubMed]
    [Google Scholar]
  37. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  38. Wellington E. M. H. , Stackebrandt E. , Sanders D. , Wolstrup J. , Jorgensen N. O. G. . ( 1992; ). Taxonomic status of Kitasatosporia, and proposed unification with Streptomyces on the basis of phenotypic and 16S rRNA analysis and emendation of Streptomyces Waksman and Henrici 1943, 339AL . . Int J Syst Bacteriol 42:, 156–160. [CrossRef] [PubMed]
    [Google Scholar]
  39. Williams S. T. , Goodfellow M. , Alderson G. , Wellington E. M. , Sneath P. H. , Sackin M. J. . ( 1983; ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129:, 1743–1813.[PubMed]
    [Google Scholar]
  40. Williams S. T. , Goodfellow M. , Alderson G. . ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL . . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams S. T. , Sharpe M. E. , Holt J. G. . . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  41. Witt D. , Stackebrandt E. . ( 1990; ). Unification of the genera Streptoverticillum and Streptomyces, and amendation of Streptomyces Waksman and Henrici 1943, 339AL . . Syst Appl Microbiol 13:, 361–371. [CrossRef]
    [Google Scholar]
  42. Zhao G. Z. , Li J. , Qin S. , Huang H. Y. , Zhu W. Y. , Xu L. H. , Li W. J. . ( 2010; ). Streptomyces artemisiae sp. nov., isolated from surface-sterilized tissue of Artemisia annua L.. Int J Syst Evol Microbiol 60:, 27–32. [CrossRef] [PubMed]
    [Google Scholar]
  43. Zheng J. , Zhang X. , Xin Y. , Han X. , Ni S. , Zhang J. . ( 2013; ). Streptomyces yaanensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 63:, 4719–4723. [CrossRef] [PubMed]
    [Google Scholar]
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