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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_9

Reclassification of as comb. nov. within gen. nov., and description of a 4-chlorophenol-degrading sulfate-reducing bacterium, sp. nov. Free

Abstract

A strictly anaerobic, mesophilic, sulfate-reducing bacterial strain (DS), isolated from river sediment contaminated with volatile organic compounds, was characterized phenotypically and phylogenetically. Cells were Gram-reaction-negative, non-motile short rods. For growth, optimum NaCl concentration was 0.9 g l, optimum temperature was 30 °C and optimum pH was 7.2. Strain DS utilized phenol, benzoate, 4-hydroxybenzoate, 4-methylphenol, 4-chlorophenol, acetate, butyrate and pyruvate as electron donors for sulfate reduction. Electron donors were completely oxidized. Strain DS did not utilize sulfite, thiosulfate or nitrate as electron acceptors. The genomic DNA G+C content of strain DS was 58.9 mol%. Major cellular fatty acids were iso-C, anteiso-C and Cω7. Phylogenetic analyses based on the 16S rRNA gene indicated its closest relatives were strains of (about 98–99 % sequence similarity) but the DNA–DNA hybridization value with Ani1 was around 40 %. Although strain DS and its relatives shared most phenotypic and chemotaxonomic characteristics, the utilization of 4-chlorophenol, the range of electron acceptors and the optimum growth conditions differed. Strain DS is closely related to strains of , but constitutes a different species within the genus. Based on phylogeny, phenotypic characteristics and chemotaxonomic characteristics, strain DS and were clearly different from strains of other species of the genus . We thus propose the reclassification of within a new genus, gen. nov., as comb. nov. We also propose sp. nov. to accommodate strain DS. The type strain is DS ( = JCM 19179 = DSM 27197).

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Funding
This study was supported by the:
  • Ministry of Education, Culture, Sports, Science, and Technology (Award 23658272 and 23310055)
© 2014 IUMS
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2014-09-01
2025-03-22
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Reclassification of Desulfobacterium anilini as Desulfatiglans anilini comb. nov. within Desulfatiglans gen. nov., and description of a 4-chlorophenol-degrading sulfate-reducing bacterium, Desulfatiglans parachlorophenolica sp. nov.
Int J Syst Evol Microbiol 64, 3081 (2014); https://doi.org/10.1099/ijs.0.064360-0
/content/journal/ijsem/10.1099/ijs.0.064360-0
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