1887

Abstract

A novel Gram-staining-negative, aerobic, rod-shaped, non-motile, reddish-orange and chemoheterotrophic bacteria, designated strain KD52, was isolated from a culture of the alga from Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD52 was a member of the family , forming a distinct lineage with ‘’ KCTC 23747. The 16S rRNA gene sequence similarity between strain KD52 and the type strains of species of the family ranged from 86 % to 89 %. Growth occurred at 20–37 °C (optimum, 28 °C), in the presence of 1–9 % (w/v) NaCl (optimum, 2.5 %) and at pH 5–8.5 (optimum, pH 6.0). The dominant fatty acids (>10 %) of strain KD52 were iso-C (33.1 %), iso-C G (14.8 %) and summed feature 3 (comprising Cω7 and/or Cω6, 13.8 %). The major polar lipids were diphosphatidylglycerol, three unidentified phospholipids, four unknown lipids and one unidentified aminolipid. The DNA G+C content was 51 mol% and the major respiratory quinone was menaquinone-7 (MK-7). On the basis of phenotypic data and phylogenetic inference, strain KD52 represents a novel species of a new genus, for which the name gen. nov., sp. nov., is proposed. The type strain is KD52 ( = MCCC 1F01213 = KCTC 32575).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.063909-0
2014-10-01
2019-11-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/10/3496.html?itemId=/content/journal/ijsem/10.1099/ijs.0.063909-0&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.. (editors) ( 1995;). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, , 3rd edn.. New York:: Wiley;.
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  3. Bowman J. P.. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  4. Daligault H., Lapidus A., Zeytun A., Nolan M., Lucas S., Del Rio T. G., Tice H., Cheng J.-F., Tapia R. et al. ( 2011;). Complete genome sequence of Haliscomenobacter hydrossis type strain (O). . Stand Genomic Sci 4:, 352–360. [CrossRef][PubMed]
    [Google Scholar]
  5. DeLong E. F.. ( 1992;). Archaea in coastal marine environments. . Proc Natl Acad Sci U S A 89:, 5685–5689. [CrossRef][PubMed]
    [Google Scholar]
  6. Hosoya S., Arunpairojana V., Suwannachart C., Kanjana-Opas A., Yokota A.. ( 2006;). Aureispira marina gen. nov., sp. nov., a gliding, arachidonic acid-containing bacterium isolated from the southern coastline of Thailand. . Int J Syst Evol Microbiol 56:, 2931–2935. [CrossRef][PubMed]
    [Google Scholar]
  7. Hosoya S., Arunpairojana V., Suwannachart C., Kanjana-Opas A., Yokota A.. ( 2007;). Aureispira maritima sp. nov., isolated from marine barnacle debris. . Int J Syst Evol Microbiol 57:, 1948–1951. [CrossRef][PubMed]
    [Google Scholar]
  8. Khan S. T., Fukunaga Y., Nakagawa Y., Harayama S.. ( 2007;). Emended descriptions of the genus Lewinella and of Lewinella cohaerens, Lewinella nigricans and Lewinella persica, and description of Lewinella lutea sp. nov. and Lewinella marina sp. nov.. Int J Syst Evol Microbiol 57:, 2946–2951. [CrossRef][PubMed]
    [Google Scholar]
  9. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. et al. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  10. Lányí B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  11. Lee S. D.. ( 2007;). Lewinella agarilytica sp. nov., a novel marine bacterium of the phylum Bacteroidetes, isolated from beach sediment. . Int J Syst Evol Microbiol 57:, 2814–2818. [CrossRef][PubMed]
    [Google Scholar]
  12. Oh H.-M., Lee K., Cho J.-C.. ( 2009;). Lewinella antarctica sp. nov., a marine bacterium isolated from Antarctic seawater. . Int J Syst Evol Microbiol 59:, 65–68. [CrossRef][PubMed]
    [Google Scholar]
  13. Reichenbach H.. ( 1989;). Genus Saprospira Gross 1911, 202. . In Bergey’s Manual of Systematic Bacteriology, vol. 3, pp. 2077–2082. Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G... Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  14. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  15. Sasser M..( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI;.
  16. Smibert R., Krieg N.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Su J., Zhou Y., Lai Q., Li X., Dong P., Yang X., Zhang B., Zhang J., Zheng X. et al. ( 2013;). Sinobacterium caligoides gen. nov., sp. nov., a new member of the family Oceanospirillaceae isolated from the South China Sea, and emended description of Amphritea japonica. . Int J Syst Evol Microbiol 63:, 2095–2100. [CrossRef][PubMed]
    [Google Scholar]
  18. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  19. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  20. van Veen W. L., van der Kooij D., Geuze E. C., van der Vlies A. W.. ( 1973;). Investigations on the sheathed bacterium Haliscomenobacter hydrossis gen.n., sp.n., isolated from activated sludge. . Antonie van Leeuwenhoek 39:, 207–216. [CrossRef][PubMed]
    [Google Scholar]
  21. Xia Y., Kong Y., Thomsen T. R., Halkjaer Nielsen P.. ( 2008;). Identification and ecophysiological characterization of epiphytic protein-hydrolyzing Saprospiraceae (“Candidatus Epiflobacter” spp.) in activated sludge. . Appl Environ Microbiol 74:, 2229–2238. [CrossRef][PubMed]
    [Google Scholar]
  22. Yoon J., Katsuta A., Kasai H.. ( 2012a;). Rubidimonas crustatorum gen. nov., sp. nov., a novel member of the family Saprospiraceae isolated from a marine crustacean. . Antonie van Leeuwenhoek 101:, 461–467. [CrossRef][PubMed]
    [Google Scholar]
  23. Yoon J., Matsuo Y., Kasai H., Yokota A.. ( 2012b;). Portibacter lacus gen. nov., sp.nov., a new member of the family Saprospiraceae isolated from a saline lake. . J Gen Appl Microbiol 58:, 191–197. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.063909-0
Loading
/content/journal/ijsem/10.1099/ijs.0.063909-0
Loading

Data & Media loading...

Supplements

Supplementary Data 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error