1887

Abstract

A novel aerobic, Gram-stain-negative, motile bacterium, designated strain BUT-10, was isolated from the sludge of a pesticide manufacturing factory in Kunshan, China. Cells were rod-shaped (0.4–0.45×0.9–1.4 µm) and colonies were white, circular with entire edges and had a smooth surface. The strain grew at 25–37 °C, at pH 6.0–8.0 and with 0–0.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain BUT-10 was a member of the genus , and showed highest sequence similarities to A8 (97.49 %), DSM 1986 (97.14 %) and FaiI3 (96.34 %). Major fatty acids (>5 %) were summed feature 8 (comprising Cω7 and/or Cω6), C and summed feature 3 (comprising Cω7 and/or Cω6). The major isoprenoid quinone was ubiquinone-10. The DNA G+C content was 71.85 mol%. Strain BUT-10 showed low DNA–DNA relatedness with A8 (15.7±2.9 %) and DSM 1986 (12.8±1.1 %). On the basis of the phenotypic, phylogenetic and genotypic data, strain BUT-10 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BUT-10 ( = CCTCC AB 2013085 = KCTC 42014).

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2015-02-01
2020-01-18
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References

  1. Abraham W.-R., Macedo A. J., Lünsdorf H., Fischer R., Pawelczyk S., Smit J., Vancanneyt M.. ( 2008;). Phylogeny by a polyphasic approach of the order Caulobacterales, proposal of Caulobacter mirabilis sp. nov., Phenylobacterium haematophilum sp. nov. and Phenylobacterium conjunctum sp. nov., and emendation of the genus Phenylobacterium. . Int J Syst Evol Microbiol 58:, 1939–1949. [CrossRef][PubMed]
    [Google Scholar]
  2. Aslam Z., Im W.-T., Ten L. N., Lee S.-T.. ( 2005;). Phenylobacterium koreense sp. nov., isolated from South Korea. . Int J Syst Evol Microbiol 55:, 2001–2005. [CrossRef][PubMed]
    [Google Scholar]
  3. Buck J. D.. ( 1982;). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44:, 992–993.[PubMed]
    [Google Scholar]
  4. Collins M. D.. ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  5. Cui X.-L., Mao P.-H., Zeng M., Li W.-J., Zhang L.-P., Xu L.-H., Jiang C.-L.. ( 2001;). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. . Int J Syst Evol Microbiol 51:, 357–363.[PubMed]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  7. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  9. Kanso S., Patel B. K.. ( 2004;). Phenylobacterium lituiforme sp. nov., a moderately thermophilic bacterium from a subsurface aquifer, and emended description of the genus Phenylobacterium. . Int J Syst Evol Microbiol 54:, 2141–2146. [CrossRef][PubMed]
    [Google Scholar]
  10. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  11. Kimura M.. ( 1983;). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  12. Lane D. L.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. R., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  13. Lingens F., Blecher R., Blecher H., Blobel F., Eberspächer J., Fröhner C., Görisch H., Görisch H., Layh G.. ( 1985;). Phenylobacterium immobile gen. nov., sp. nov., a gram-negative bacterium that degrades the herbicide chloridazon. . Int J Syst Bacteriol 35:, 26–39. [CrossRef]
    [Google Scholar]
  14. Mesbah M., Whitman W. B.. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479:, 297–306. [CrossRef][PubMed]
    [Google Scholar]
  15. Oh Y.-S., Roh D.-H.. ( 2012;). Phenylobacterium muchangponense sp. nov., isolated from beach soil, and emended description of the genus Phenylobacterium. . Int J Syst Evol Microbiol 62:, 977–983. [CrossRef][PubMed]
    [Google Scholar]
  16. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  17. Sambrook J., Russell D. W.. ( 2001;). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory Press;.
    [Google Scholar]
  18. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  19. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S.. ( 2001;). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:, 1639–1652. [CrossRef][PubMed]
    [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  22. Tiago I., Mendes V., Pires C., Morais P. V., Veríssimo A.. ( 2005;). Phenylobacterium falsum sp. nov., an Alphaproteobacterium isolated from a nonsaline alkaline groundwater, and emended description of the genus Phenylobacterium. . Syst Appl Microbiol 28:, 295–302. [CrossRef][PubMed]
    [Google Scholar]
  23. Tindall B.. ( 1990;). Lipid composition of Halobacterium lacusprofundi.. FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  24. Wayne L., Brenner D., Colwell R., Grimont P., Kandler O., Krichevsky M., Moore L., Moore W., Murray R.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  25. Weon H.-Y., Kim B.-Y., Kwon S.-W., Go S.-J., Koo B.-S., Stackebrandt E.. ( 2008;). Phenylobacterium composti sp. nov., isolated from cotton waste compost in Korea. . Int J Syst Evol Microbiol 58:, 2301–2304. [CrossRef][PubMed]
    [Google Scholar]
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