1887

Abstract

A novel aerobic, Gram-stain-negative, motile bacterium, designated strain BUT-10, was isolated from the sludge of a pesticide manufacturing factory in Kunshan, China. Cells were rod-shaped (0.4–0.45×0.9–1.4 µm) and colonies were white, circular with entire edges and had a smooth surface. The strain grew at 25–37 °C, at pH 6.0–8.0 and with 0–0.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain BUT-10 was a member of the genus , and showed highest sequence similarities to A8 (97.49 %), DSM 1986 (97.14 %) and FaiI3 (96.34 %). Major fatty acids (>5 %) were summed feature 8 (comprising Cω7 and/or Cω6), C and summed feature 3 (comprising Cω7 and/or Cω6). The major isoprenoid quinone was ubiquinone-10. The DNA G+C content was 71.85 mol%. Strain BUT-10 showed low DNA–DNA relatedness with A8 (15.7±2.9 %) and DSM 1986 (12.8±1.1 %). On the basis of the phenotypic, phylogenetic and genotypic data, strain BUT-10 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BUT-10 ( = CCTCC AB 2013085 = KCTC 42014).

Funding
This study was supported by the:
  • National Science and Technology Support Plan (Award 2013AA102804 and 2012BAD15B03)
  • National Natural Science Foundation of China (Award 31270157)
  • Fundamental Research Funds for the Central Universities (Award KYZ201122)
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2015-02-01
2021-10-21
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