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Abstract

A Gram-staining-positive, non-spore-forming, rod-shaped, non-motile and aerobic bacterium, strain THG-e54, was isolated from soil of a flowerbed in Bucheon, South Korea. The taxonomic position of this bacterium was determined in an investigation based on a polyphasic approach. 16S rRNA gene sequence analysis revealed a clear affiliation of strain THG-e54 with the genus . Strain THG-e54 showed the closest phylogenetic relationship with DSM 20308 with a 16S rRNA gene sequence similarity of 98.6 %. The G+C content of the genomic DNA was 71.0 mol%. The major cellular fatty acids of strain THG-e54 were iso-C and iso-C, and the predominant menaquinone was menaquinone MK-8(H). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unidentified amino-containing phosphoglycolipid, unidentified phospholipids, an unidentified aminolipid and an unidentified aminophospholipid. The cell-wall peptidoglycan type was -diaminopimelic acid and the cell-wall sugars were glucose and ribose. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 35.2 %) between strain THG-e54 and its five closest relatives. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain THG-e54 could be clearly distinguished from closely related recognized members of the genus . Therefore the results of this study indicated the existence of a novel species of the genus , for which we propose the name sp. nov., with strain THG-e54 ( = KACC 17589 = JCM 19342) as the type strain.

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2014-10-01
2021-03-06
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