1887

Abstract

A Gram-staining-negative, heterotrophic, facultatively anaerobic bacterium, designated S1, was isolated from the culture pond in a recirculating mariculture system in Tianjin, China. The taxonomy of strain S1 was studied by using a polyphasic approach. Cells of strain S1 were non-spore-forming, curved rods, 0.4–0.6 µm wide and 1.2–2.0 µm long, and motile by means of a single polar flagellum. The strain was positive for oxidase and catalase activities. Strain S1 was able to grow at 4–30 °C (optimum, 25 °C), at pH 5.5–10.0 (optimum, pH 6.5–7.5) and in the presence of 1–5 % (w/v) NaCl (optimum, 2 %). Strain S1 contained Q-8 as the sole respiratory quinone and Cω7/Cω6 and C as the predominant cellular fatty acids. The genomic DNA G+C content was 40.1 mol% ( ). Phylogenetic analysis based on 16S rRNA gene sequences placed strain S1 in the genus , and it formed a distinct lineage in the phylogenetic tree together with MA1-3, SMK-10, 537 and BCw111, with 97.7, 96.1, 95.9 and 95.0 % 16S rRNA gene sequence similarity to these strains, respectively. DNA–DNA relatedness of strain S1 to MA1-3 was 23.5±3.6 %. On the basis of the phylogenetic and phenotypic evidence, strain S1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S1 ( = CGMCC 1.12165 = JCM 18479).

Funding
This study was supported by the:
  • , Chinese Academy of Sciences , (Award KSCS2-YW-G-055-01)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.063305-0
2014-12-01
2021-03-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/12/3926.html?itemId=/content/journal/ijsem/10.1099/ijs.0.063305-0&mimeType=html&fmt=ahah

References

  1. Bowman J. P., Gosink J. J., Mccammon S. A., Lewis T. E., Nichols D. S., Nichols P. D., Skerratt J. H., Staley J. T., Mcmeekin T. A. ( 1998 ). Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22:ω63). . Int J Syst Bacteriol 48, 11711180. [CrossRef]
    [Google Scholar]
  2. Choi E. J., Kwon H. C., Koh H. Y., Kim Y. S., Yang H. O. ( 2010 ). Colwellia asteriadis sp. nov., a marine bacterium isolated from the starfish Asterias amurensis . . Int J Syst Evol Microbiol 60, 19521957. [CrossRef] [PubMed]
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [CrossRef] [PubMed]
    [Google Scholar]
  4. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [CrossRef] [PubMed]
    [Google Scholar]
  6. Deming J. W., Somers L. K., Straube W. L., Swartz D. G., Macdonell M. T. ( 1988 ). Isolation of an obligately barophilic bacterium and description of a new genus, Colwellia gen. nov.. Syst Appl Microbiol 10, 152160. [CrossRef]
    [Google Scholar]
  7. Dong X.-Z., Cai M.-Y. ( 2001 ). Determinative Manual for Routine Bacteriology. Beijing: Scientific Press: (English translation).
    [Google Scholar]
  8. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  9. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  10. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors ) ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  11. Huß V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [CrossRef] [PubMed]
    [Google Scholar]
  12. Jung S.-Y., Oh T.-K., Yoon J.-H. ( 2006 ). Colwellia aestuarii sp. nov., isolated from a tidal flat sediment in Korea. . Int J Syst Evol Microbiol 56, 3337. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kim S. B., Falconer C., Williams E., Goodfellow M. ( 1998 ). Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. . Int J Syst Bacteriol 48, 5968. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kim Y.-O., Park S., Nam B.-H., Jung Y.-T., Kim D.-G., Yoon J.-H. ( 2013 ). Colwellia meonggei sp. nov., a novel gammaproteobacterium isolated from sea squirt Halocynthia roretzi . . Antonie van Leeuwenhoek 104, 10211027. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  17. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic from micro-organisms. . J Mol Biol 3, 208218. [CrossRef]
    [Google Scholar]
  18. Nogi Y., Hosoya S., Kato C., Horikoshi K. ( 2004 ). Colwellia piezophila sp. nov., a novel piezophilic species from deep-sea sediments of the Japan Trench. . Int J Syst Evol Microbiol 54, 16271631. [CrossRef] [PubMed]
    [Google Scholar]
  19. Nokhal T. H., Schlegel H. G. ( 1983 ). Taxonomic study of Paracoccus denitrificans . . Int J Syst Bacteriol 33, 2637. [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  21. Sly L. I., Blackall L. L., Kraat P. C., Tian-Shen T., Sangkhobol V. ( 1986 ). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. . J Microbiol Methods 5, 139156. [CrossRef]
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  24. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989 ). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16, 176178. [CrossRef]
    [Google Scholar]
  25. Yu Y., Li H.-R., Zeng Y.-X. ( 2011 ). Colwellia chukchiensis sp. nov., a psychrotolerant bacterium isolated from the Arctic Ocean. . Int J Syst Evol Microbiol 61, 850853. [CrossRef] [PubMed]
    [Google Scholar]
  26. Yumoto I., Kawasaki K., Iwata H., Matsuyama H., Okuyama H. ( 1998 ). Assignment of Vibrio sp. strain ABE-1 to Colwellia maris sp. nov., a new psychrophilic bacterium. . Int J Syst Bacteriol 48, 13571362. [CrossRef] [PubMed]
    [Google Scholar]
  27. Zhang D. C., Yu Y., Xin Y. H., Liu H. C., Zhou P. J., Zhou Y. G. ( 2008 ). Colwellia polaris sp. nov., a psychrotolerant bacterium isolated from Arctic sea ice. . Int J Syst Evol Microbiol 58, 19311934. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.063305-0
Loading
/content/journal/ijsem/10.1099/ijs.0.063305-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error