1887

Abstract

A Gram-staining-negative, heterotrophic, facultatively anaerobic bacterium, designated S1, was isolated from the culture pond in a recirculating mariculture system in Tianjin, China. The taxonomy of strain S1 was studied by using a polyphasic approach. Cells of strain S1 were non-spore-forming, curved rods, 0.4–0.6 µm wide and 1.2–2.0 µm long, and motile by means of a single polar flagellum. The strain was positive for oxidase and catalase activities. Strain S1 was able to grow at 4–30 °C (optimum, 25 °C), at pH 5.5–10.0 (optimum, pH 6.5–7.5) and in the presence of 1–5 % (w/v) NaCl (optimum, 2 %). Strain S1 contained Q-8 as the sole respiratory quinone and Cω7/Cω6 and C as the predominant cellular fatty acids. The genomic DNA G+C content was 40.1 mol% ( ). Phylogenetic analysis based on 16S rRNA gene sequences placed strain S1 in the genus , and it formed a distinct lineage in the phylogenetic tree together with MA1-3, SMK-10, 537 and BCw111, with 97.7, 96.1, 95.9 and 95.0 % 16S rRNA gene sequence similarity to these strains, respectively. DNA–DNA relatedness of strain S1 to MA1-3 was 23.5±3.6 %. On the basis of the phylogenetic and phenotypic evidence, strain S1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S1 ( = CGMCC 1.12165 = JCM 18479).

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2014-12-01
2019-11-22
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